SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003070
Component protein type	TssI
T6SS ID (Type)	T6SS00301 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853
Replicon	chromosome
Sequence	Protein sequence (730 a.a.); Nucleotide sequence (2190 bp)
Reference	[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904 [2] Pezoa D, Blondel CJ, Silva CA, et al. Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts. Vet Res. 2014 Jan 9;45:2. doi: 10.1186/1297-9716-45-2. PMID: 24405577

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	SED_RS01900
Coordinate (Strand)	329155..331344 (+)
NCBI ID	445933675
RefSeq	NC_011205
Uniprot ID	UPI00016A0C38
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	6.6e-103	121..429
Phage_base_V	PF04717.14	2.1e-13	484..551

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-729	MSFVSTNNKSGMGGLTTTTPPITGESGGVTADSVAGSVADAAESAVEQAAGSLFGALPEPSGLVKAAVAAAQAAAAAGMAQDAVSAIVSAVADGPGAHNVTVSGSAVPPGALLFASLDGGETLSELFSYVVQLKTPDTLNLGYVSPAANLPLKPMVGKDLCVNIELDGGGKRHISGLVTAARVVGHEGRSVTYELRMEPWLKLLTHTSDYKAFQNKTVVDILDEVLAEYPYPVEKRLVESYPVRTWQVQYGETDFDFLQRLMQEWGIYWWFEHSEDSHTLVLADAISAHKACPDSPLVEWHQEGLKLDKEFIHTITANESLRTGQWVLDDFDFTKPRSLLANTVANPRETGHATYEHYEWPGDYFDKSEGEMLTRIRMEAQRSPGSRVLGAGNIRTLMTGYTFTLENYPTAEVNQEYLLMQTLLFVQDNAQHSGQDQHFTFSTRFELHPTREVFRPQRTVSKPHTKGPQSAIVTGPSGQEIWTDQYGRVKVQFGWDRYGKMDENSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPGMASQSGIFSHSLYGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVDRTKTIIKNETNSIGEDRNTTVTKNDGLSVKLAQTINIGTTYRLDVGDQFTLRCGNAALVLHKDGSIEFCGKQLMLHTSDVMQLIGKGIDMNPDGGTAVTADDIAPLPTSE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8920	-	-

Protein sequence: 730 a.a.

						>T6CP003070 NC_011205:329155-331344 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TssI]
MSFVSTNNKSGMGGLTTTTPPITGESGGVTADSVAGSVADAAESAVEQAAGSLFGALPEPSGLVKAAVAAAQAAAAAGMA
QDAVSAIVSAVADGPGAHNVTVSGSAVPPGALLFASLDGGETLSELFSYVVQLKTPDTLNLGYVSPAANLPLKPMVGKDL
CVNIELDGGGKRHISGLVTAARVVGHEGRSVTYELRMEPWLKLLTHTSDYKAFQNKTVVDILDEVLAEYPYPVEKRLVES
YPVRTWQVQYGETDFDFLQRLMQEWGIYWWFEHSEDSHTLVLADAISAHKACPDSPLVEWHQEGLKLDKEFIHTITANES
LRTGQWVLDDFDFTKPRSLLANTVANPRETGHATYEHYEWPGDYFDKSEGEMLTRIRMEAQRSPGSRVLGAGNIRTLMTG
YTFTLENYPTAEVNQEYLLMQTLLFVQDNAQHSGQDQHFTFSTRFELHPTREVFRPQRTVSKPHTKGPQSAIVTGPSGQE
IWTDQYGRVKVQFGWDRYGKMDENSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWG
LPGMASQSGIFSHSLYGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVDRTKTIIKNETNSIGEDRNTTV
TKNDGLSVKLAQTINIGTTYRLDVGDQFTLRCGNAALVLHKDGSIEFCGKQLMLHTSDVMQLIGKGIDMNPDGGTAVTAD
DIAPLPTSE*					

Nucleotide sequence: 2190 bp

						>T6CP003070 NC_011205:329155-331344 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TssI]
ATGAGTTTTGTATCCACAAATAATAAATCCGGTATGGGAGGGCTGACGACAACCACGCCGCCGATAACCGGAGAAAGTGG
CGGTGTCACCGCAGATTCAGTCGCCGGAAGCGTGGCAGATGCGGCGGAATCCGCCGTGGAACAGGCTGCGGGATCGCTGT
TTGGCGCATTGCCGGAGCCATCAGGACTGGTGAAAGCCGCGGTAGCAGCGGCGCAGGCTGCCGCCGCCGCAGGTATGGCG
CAGGATGCGGTATCGGCCATCGTCTCTGCTGTTGCAGACGGGCCGGGGGCGCATAATGTGACGGTCAGCGGCAGCGCCGT
ACCGCCGGGCGCATTACTGTTCGCCAGCCTGGACGGCGGCGAAACATTAAGTGAACTGTTCAGCTATGTGGTACAGCTAA
AAACGCCCGACACCCTGAATCTGGGCTATGTCTCCCCGGCGGCCAACCTGCCGCTCAAACCGATGGTGGGCAAAGATCTG
TGCGTCAACATCGAACTGGATGGTGGCGGTAAACGACATATCAGCGGGCTGGTCACGGCGGCGCGGGTGGTGGGCCATGA
AGGGCGTTCGGTTACCTATGAGCTGCGTATGGAGCCGTGGCTGAAACTGCTGACCCATACCAGCGACTACAAAGCATTCC
AGAATAAAACCGTGGTGGATATTCTGGATGAGGTTCTGGCGGAATACCCCTACCCGGTGGAAAAGCGGCTGGTGGAAAGC
TACCCGGTACGCACCTGGCAGGTGCAGTACGGTGAAACTGATTTTGATTTTCTTCAGCGACTGATGCAGGAGTGGGGCAT
CTACTGGTGGTTTGAGCACAGCGAGGACAGCCACACGCTGGTGCTGGCGGATGCCATCAGCGCCCACAAAGCATGTCCGG
ACTCGCCGCTGGTCGAGTGGCACCAGGAAGGGCTGAAGCTGGACAAGGAGTTTATCCACACTATCACGGCAAACGAGAGT
CTGCGGACTGGCCAGTGGGTGCTGGATGATTTCGATTTTACGAAGCCACGTTCATTGCTGGCAAACACGGTGGCAAACCC
GCGTGAAACCGGTCATGCCACCTACGAGCATTATGAGTGGCCGGGAGACTACTTCGACAAGAGTGAAGGCGAGATGCTGA
CGCGCATTCGTATGGAAGCGCAGCGCAGCCCCGGCAGCCGGGTGCTGGGTGCCGGGAATATTCGTACGCTGATGACAGGC
TATACCTTCACGCTGGAAAACTATCCCACCGCCGAAGTCAATCAGGAATATCTGCTGATGCAGACCTTGCTGTTTGTGCA
GGACAACGCGCAGCACAGCGGGCAGGACCAGCACTTTACCTTTTCCACCCGTTTTGAACTGCACCCCACCCGCGAGGTGT
TCCGCCCGCAGCGGACGGTGAGCAAACCCCACACCAAAGGGCCGCAGAGCGCCATTGTCACCGGCCCGTCGGGCCAGGAA
ATCTGGACGGATCAGTACGGGCGGGTAAAGGTACAGTTTGGCTGGGATCGCTACGGCAAAATGGATGAAAACAGCTCCTG
CTGGATACGCGTCAGCTACCCATGGGCGGGCAAAGGCTTCGGGATGATCCAGATCCCGCGTATCGGCCAGGAAGTGCTGG
TGGATTTCAAAAACGGCGATCCGGATCTGCCGATCATCGTGGGGCGTACCTACAACCAGGACACCATGCCGCCGTGGGGA
CTGCCGGGAATGGCGTCGCAGAGCGGGATCTTCAGCCACTCGCTGTATGGCGGGCCAACGAACGGCAACATGCTGCGTTT
TGACGACAAAACGGGCGCGGAGGAAGTGAAGTTCCACGCGGAAAAAGATCTCAACACCACGGTGAAGAATAATGAAACGC
ATACGGTTATGGTGGATCGCACTAAAACCATTATTAAAAATGAAACCAACAGTATTGGTGAGGACAGAAACACCACGGTA
ACGAAGAATGACGGCCTTTCCGTAAAACTGGCGCAGACGATCAATATCGGCACCACTTATCGTTTAGATGTTGGCGATCA
ATTCACACTTCGCTGCGGCAATGCGGCGCTTGTTTTACATAAGGACGGCTCCATTGAGTTTTGTGGCAAGCAACTGATGT
TACATACCAGCGATGTCATGCAACTGATTGGTAAAGGTATTGATATGAACCCGGATGGCGGCACAGCCGTAACCGCCGAT
GATATTGCCCCCCTTCCCACCTCTGAGTGA					