SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP003069
Component protein type	TssM
T6SS ID (Type)	T6SS00301 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853
Replicon	chromosome
Sequence	Protein sequence (1290 a.a.); Nucleotide sequence (3870 bp)
Reference	[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904 [2] Pezoa D, Blondel CJ, Silva CA, et al. Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts. Vet Res. 2014 Jan 9;45:2. doi: 10.1186/1297-9716-45-2. PMID: 24405577

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	SED_RS01880
Coordinate (Strand)	323143..327012 (+)
NCBI ID	447091711
RefSeq	NC_011205
Uniprot ID	M7RBF4_SALDU
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	8e-85	265..538
IcmF-related	PF06761.14	3.4e-77	590..915
IcmF_C	PF06744.14	6.9e-25	1163..1268

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	MQKFLSLLFSRR
Transmembrane helix	13-35	ALAVVGVLVLALLVWFVGPLVSF
Outside	36-39	DTLR
Transmembrane helix	40-62	PLASVGSRVVTIALLLMLLVLWL
Inside	63-68	VNWSMS
Transmembrane helix	69-88	IIGISVLCLAIGFVTPLLAL
Outside	89-97	GDVHPFAPL
Transmembrane helix	98-120	WVRLTLIGFILLMYALYGLYRLW
Inside	121-529	RALRMDEQLLRRFLHPRGEEVPVAGEIKADLRTVNHIVTQAIRQLRQLRVDMPGWRKIFEGKRFLYELPWFMVVGSPGDGKTTALLNTGLQFPLAEQMEQTSRILTVPGGGTLHCDWWFTNEAVLIDTAGRYARHDDGGEVSAAQRNAGEWQGFLGLLRKHRPGAPLNGVILTLNVADLTAQSPAERLAACAALRARLAELRETLGIRFPVYLVVTKMDLLPGFSEYFRTLTSHLRAQIWGFTLPYSRRRKAGDPQALHAACAQELARLTLRLDQGLDTRLQEEYDLKSRQRLYTFPREFAALGEPLLEAIEQIFLDSKFDATQLNNTLRGVFFTSAAQAQADAVADQLSIWQRFVRAIRTARGESSASLPHALPDGNRSYFLHDLLTQFIFREAHLVEPNLQWAWRYR
Transmembrane helix	530-547	LLRLGGHLLVLVLAFLLW
Outside	548-1289	QGMQTSQQTNGDYLNEISARATRLDGEVKAYTGKPAMAPVPALLDSARELSAWPELDPDAPPLTWRYGLYSVPPVTDSVASLYNRLLDQLLLPPLVKRMEYVLADAIARQDSKAAYDALRIYLLLNLDKDHEDKYNAAEIQSWVVNDLGNSDSVAGFGGRAAVLTHIEALFDGSRVVHSPYEKNEALIRQARAFLDGHTSTERIYARALAAMESEAPQEFTLVRAVGADAGTVFVRSNGAPLDRGVPGIFTREGYRELFDKRLPEFVAAATANDGWVMGRESTPKKLTDSLRSQIPGQEQSVAREVRRLYLTEYARRWQDFLDSIHSINSAGEEGSSGLAYDLQVLRTLASPDSPLMRLGKAVVEQTTLVPPPDPQARQKQLAQRASGNAGKVVQTAKLFQDIHPEERLEKTLVDDRFAALREVIAGRTDGGQSGGGTMQIASLLTMLNEYYTQLTIADSALAAGTLPARITAADKLQLEAAKLPAPLKNILLDLTKQGTRKINAGTGDVLNTQMEAMMGDDCRDAIDGRYPFADSPQEVSAEDFNRIFASGGVLDAFWSKQLAPLADTASDPWRYKPTEGNMTLQGPDLTPFQQAKQIRSVFFNSEGGKKFSWSMQISVVDMDPAITELVIDIDGQVLRYAHGPDRPLKVTWPGPRNGSMAEITASPRIRQDTSTLLTGGPWALFHLLDAGMVQETAVRGRQLVEYDFDGRRVVLEITAGRDFNPVSRELLQNFSCPARAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9933	-	-

Protein sequence: 1290 a.a.

						>T6CP003069 NC_011205:323143-327012 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TssM]
MQKFLSLLFSRRALAVVGVLVLALLVWFVGPLVSFDTLRPLASVGSRVVTIALLLMLLVLWLVNWSMSIIGISVLCLAIG
FVTPLLALGDVHPFAPLWVRLTLIGFILLMYALYGLYRLWRALRMDEQLLRRFLHPRGEEVPVAGEIKADLRTVNHIVTQ
AIRQLRQLRVDMPGWRKIFEGKRFLYELPWFMVVGSPGDGKTTALLNTGLQFPLAEQMEQTSRILTVPGGGTLHCDWWFT
NEAVLIDTAGRYARHDDGGEVSAAQRNAGEWQGFLGLLRKHRPGAPLNGVILTLNVADLTAQSPAERLAACAALRARLAE
LRETLGIRFPVYLVVTKMDLLPGFSEYFRTLTSHLRAQIWGFTLPYSRRRKAGDPQALHAACAQELARLTLRLDQGLDTR
LQEEYDLKSRQRLYTFPREFAALGEPLLEAIEQIFLDSKFDATQLNNTLRGVFFTSAAQAQADAVADQLSIWQRFVRAIR
TARGESSASLPHALPDGNRSYFLHDLLTQFIFREAHLVEPNLQWAWRYRLLRLGGHLLVLVLAFLLWQGMQTSQQTNGDY
LNEISARATRLDGEVKAYTGKPAMAPVPALLDSARELSAWPELDPDAPPLTWRYGLYSVPPVTDSVASLYNRLLDQLLLP
PLVKRMEYVLADAIARQDSKAAYDALRIYLLLNLDKDHEDKYNAAEIQSWVVNDLGNSDSVAGFGGRAAVLTHIEALFDG
SRVVHSPYEKNEALIRQARAFLDGHTSTERIYARALAAMESEAPQEFTLVRAVGADAGTVFVRSNGAPLDRGVPGIFTRE
GYRELFDKRLPEFVAAATANDGWVMGRESTPKKLTDSLRSQIPGQEQSVAREVRRLYLTEYARRWQDFLDSIHSINSAGE
EGSSGLAYDLQVLRTLASPDSPLMRLGKAVVEQTTLVPPPDPQARQKQLAQRASGNAGKVVQTAKLFQDIHPEERLEKTL
VDDRFAALREVIAGRTDGGQSGGGTMQIASLLTMLNEYYTQLTIADSALAAGTLPARITAADKLQLEAAKLPAPLKNILL
DLTKQGTRKINAGTGDVLNTQMEAMMGDDCRDAIDGRYPFADSPQEVSAEDFNRIFASGGVLDAFWSKQLAPLADTASDP
WRYKPTEGNMTLQGPDLTPFQQAKQIRSVFFNSEGGKKFSWSMQISVVDMDPAITELVIDIDGQVLRYAHGPDRPLKVTW
PGPRNGSMAEITASPRIRQDTSTLLTGGPWALFHLLDAGMVQETAVRGRQLVEYDFDGRRVVLEITAGRDFNPVSRELLQ
NFSCPARAL*					

Nucleotide sequence: 3870 bp

						>T6CP003069 NC_011205:323143-327012 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TssM]
ATGCAAAAATTTCTTAGTCTGCTTTTTTCCCGGCGCGCGCTGGCAGTTGTGGGCGTTCTGGTTCTGGCGCTGCTGGTCTG
GTTTGTCGGGCCGCTGGTGTCATTTGATACCCTGCGCCCGCTGGCCTCCGTGGGTAGCCGGGTAGTGACCATTGCCCTGT
TGCTGATGCTGCTGGTACTGTGGCTGGTCAACTGGTCGATGAGTATCATCGGCATCAGTGTCCTGTGCCTGGCGATTGGC
TTCGTCACACCGCTGCTGGCCCTGGGCGATGTCCATCCGTTTGCGCCGCTGTGGGTCCGCCTGACATTAATTGGTTTCAT
CCTGCTGATGTACGCGCTGTACGGCCTGTACCGGCTGTGGCGTGCGCTGCGTATGGATGAACAACTGCTGCGTCGCTTCC
TGCATCCGCGCGGGGAAGAGGTACCGGTGGCAGGCGAGATCAAAGCCGACCTGCGCACCGTCAACCATATTGTCACGCAG
GCCATCCGGCAGCTGCGGCAGTTGCGGGTGGATATGCCTGGCTGGCGTAAGATCTTCGAGGGAAAACGCTTTCTGTATGA
GCTGCCGTGGTTCATGGTGGTCGGCAGTCCCGGCGACGGCAAAACCACGGCCCTGCTGAACACCGGATTGCAGTTCCCGC
TGGCGGAGCAAATGGAGCAGACTTCGCGCATCCTGACAGTACCGGGTGGCGGCACGCTACACTGCGACTGGTGGTTTACC
AACGAAGCGGTGTTGATTGATACCGCCGGACGCTACGCCCGCCACGATGACGGCGGAGAAGTGAGCGCCGCGCAGCGTAA
CGCCGGAGAGTGGCAGGGCTTTCTCGGTCTGCTGCGTAAACATCGCCCCGGCGCGCCGCTTAACGGCGTGATCCTGACGC
TAAACGTGGCGGATTTAACCGCACAGTCACCGGCGGAACGCCTGGCGGCCTGCGCCGCTCTGCGGGCGCGACTGGCAGAA
CTGCGCGAGACCCTGGGGATTCGCTTTCCGGTCTATCTGGTGGTCACCAAAATGGATTTGTTGCCGGGGTTCAGCGAGTA
TTTTCGCACGCTGACCAGCCATCTTCGCGCACAAATCTGGGGCTTTACGTTGCCGTACAGCCGCAGGCGAAAAGCGGGCG
ACCCGCAGGCGCTGCACGCCGCCTGCGCGCAGGAGCTGGCGCGCCTGACGCTGCGGCTGGATCAGGGACTGGATACCCGG
TTACAGGAAGAGTACGACCTTAAAAGCCGCCAGCGGCTGTATACCTTCCCGCGTGAGTTCGCCGCCCTCGGCGAGCCGTT
GCTGGAGGCTATTGAACAGATCTTCCTCGATTCAAAATTCGATGCCACGCAACTGAATAACACGCTGCGCGGGGTGTTTT
TCACCAGCGCCGCGCAGGCGCAGGCCGACGCCGTGGCCGACCAGTTGAGTATCTGGCAGCGCTTTGTCCGGGCGATAAGA
ACCGCCCGTGGCGAATCATCCGCCTCTCTCCCGCACGCTCTGCCGGACGGCAACCGCAGCTACTTTCTGCATGACCTGCT
GACACAGTTTATTTTCCGTGAAGCGCACCTGGTGGAGCCAAACCTCCAGTGGGCCTGGCGTTACCGCCTGCTGCGCCTCG
GCGGGCACCTGCTGGTACTGGTGCTGGCATTCCTGTTGTGGCAGGGGATGCAGACCAGCCAGCAGACCAACGGCGACTAT
CTGAATGAAATCAGCGCCCGCGCGACCCGGCTGGACGGTGAAGTGAAAGCCTACACCGGTAAACCGGCGATGGCTCCCGT
CCCGGCACTGCTGGATAGCGCAAGGGAACTGTCCGCCTGGCCGGAACTGGACCCGGACGCGCCGCCGCTGACCTGGCGCT
ACGGCCTGTACAGCGTACCGCCGGTAACCGACAGCGTGGCGTCGCTGTACAACCGTCTGCTGGATCAACTGCTGCTGCCG
CCGCTGGTGAAACGGATGGAGTATGTGCTGGCGGACGCCATTGCCCGTCAGGACAGTAAAGCTGCGTATGATGCCCTGCG
CATCTATCTGCTACTGAATCTGGATAAAGATCACGAAGATAAATACAACGCGGCGGAGATCCAGTCGTGGGTGGTTAACG
ATCTGGGGAACAGCGACAGCGTGGCCGGGTTCGGCGGGCGCGCCGCCGTGCTGACGCATATCGAAGCGCTGTTTGACGGC
AGCCGGGTGGTGCATTCACCGTATGAGAAAAATGAGGCACTGATCCGCCAGGCGCGGGCATTCCTCGACGGTCACACCAG
TACCGAGCGTATCTATGCGCGGGCGCTGGCGGCGATGGAGAGCGAAGCGCCGCAGGAGTTCACGCTGGTACGCGCCGTCG
GCGCGGATGCGGGAACGGTGTTTGTGCGTAGCAACGGCGCGCCGCTGGATCGGGGCGTGCCGGGTATTTTTACCCGTGAA
GGATACCGGGAGCTGTTCGACAAACGATTACCGGAATTTGTGGCGGCGGCGACGGCGAACGATGGCTGGGTGATGGGCCG
GGAGAGTACGCCAAAAAAGCTGACTGACAGCCTGCGCAGCCAGATACCGGGGCAGGAGCAGTCTGTCGCCCGCGAAGTCC
GCCGTTTGTACCTGACGGAATATGCCCGCCGCTGGCAGGATTTTCTGGACAGTATCCATAGTATCAACAGTGCCGGGGAA
GAGGGCAGTTCCGGCCTGGCCTATGATTTACAGGTGCTGCGCACCCTGGCGTCGCCGGACTCACCGCTGATGCGGCTGGG
AAAAGCGGTGGTGGAGCAGACCACGCTGGTGCCGCCGCCGGACCCGCAGGCCAGACAGAAACAACTGGCGCAGCGCGCAT
CCGGTAACGCGGGGAAAGTGGTACAGACGGCAAAACTGTTCCAGGATATTCACCCGGAAGAACGGCTGGAAAAAACGCTG
GTGGATGATCGTTTCGCCGCGCTGCGCGAGGTCATTGCCGGGCGGACGGACGGCGGACAGAGCGGCGGTGGGACGATGCA
GATCGCTTCGCTGCTGACCATGCTCAACGAGTATTACACCCAACTGACCATTGCTGATAGCGCGCTGGCGGCGGGGACGT
TGCCAGCGCGCATTACTGCCGCAGACAAGTTGCAACTGGAGGCGGCGAAACTGCCTGCGCCGCTGAAAAACATCCTGCTG
GATCTGACGAAGCAGGGAACGCGCAAAATCAACGCCGGGACCGGCGACGTGCTGAACACCCAGATGGAGGCGATGATGGG
CGACGACTGCCGTGACGCCATCGACGGGCGTTATCCGTTCGCCGACAGCCCGCAGGAGGTCAGCGCCGAGGACTTTAACC
GCATCTTCGCCAGCGGCGGCGTACTGGATGCTTTCTGGAGCAAACAACTGGCTCCGCTGGCGGACACCGCCAGCGACCCG
TGGCGCTACAAACCGACCGAAGGCAACATGACATTGCAGGGGCCGGATTTAACGCCGTTTCAGCAGGCGAAGCAGATCCG
CAGCGTGTTTTTTAACAGCGAGGGCGGGAAAAAATTTTCCTGGTCGATGCAGATTAGCGTGGTGGATATGGACCCGGCCA
TCACGGAACTGGTGATTGATATCGACGGCCAGGTGCTGCGCTACGCCCATGGTCCGGACCGGCCCCTGAAAGTGACGTGG
CCGGGACCGCGCAACGGCTCGATGGCGGAAATCACCGCCAGCCCGCGTATTCGCCAGGACACCTCAACGCTACTGACCGG
CGGGCCGTGGGCGCTGTTTCATCTGCTGGACGCCGGAATGGTACAGGAAACGGCGGTGCGGGGGCGCCAATTGGTGGAAT
ATGATTTTGACGGTCGCCGGGTGGTGCTGGAAATCACTGCCGGGCGGGATTTTAACCCGGTCAGCCGGGAGCTGTTGCAG
AACTTCAGTTGCCCGGCGAGGGCGCTGTAA					