Detailed information of component protein


Summary

Component ID T6CP002766
Component protein type TssL
T6SS ID (Type) T6SS00276 insolico (Type Unknown)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (430 a.a.); Nucleotide sequence (1290 bp)
Reference
-
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS34850
Coordinate (Strand) 1675944..1677233 (-)
NCBI ID WP_012406179.1
RefSeq NC_010625
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.1e-66 54..262
OmpA PF00691.22 5.5e-15 323..421
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-240 MSDKAALLRGTARDTEAQSYADAAVSGQEGLPYEVPLGEPALERLTKVRSAANPLLEAARVLLRSQADIPEMDLGAKGVIPTLRQLLLDEMKTFQKLCEQTSIRRDHMIGARYCLCTALDEAVMETPWGRTGDNGVSWGTVGLATNFHEDRDGGTKIYLLVARLMQDPDQHDDLLEVIYHILSFGFEGRYRSERDGHRKHEAVRQRIYNEIMARRPPVPVELSPHARSDVPPRRQSFYDF
Transmembrane helix 241-260 PVWITVTVLSLILLGMWGYF
Inside 261-429 KYQLVTRADDIQKQIGDIARMTPPPAPPVLHLKTLLKDEIAAGTVSVDEDAKHSAVTFRGDSMFPPGGVAVNTSMGPLIAKIAAEIVKVPGKVTVLGYTDNLPIHSHAFASNDALSAERATQVLQMLQADGVPASRLEAMGKGEADPVADNSTPQGRAQNRRVEIHVAE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9848 - -


  Protein sequence: 430 a.a.    

>T6CP002766 NC_010625:c1677233-1675944 [Paraburkholderia phymatum STM815] [TssL]
MSDKAALLRGTARDTEAQSYADAAVSGQEGLPYEVPLGEPALERLTKVRSAANPLLEAARVLLRSQADIPEMDLGAKGVI
PTLRQLLLDEMKTFQKLCEQTSIRRDHMIGARYCLCTALDEAVMETPWGRTGDNGVSWGTVGLATNFHEDRDGGTKIYLL
VARLMQDPDQHDDLLEVIYHILSFGFEGRYRSERDGHRKHEAVRQRIYNEIMARRPPVPVELSPHARSDVPPRRQSFYDF
PVWITVTVLSLILLGMWGYFKYQLVTRADDIQKQIGDIARMTPPPAPPVLHLKTLLKDEIAAGTVSVDEDAKHSAVTFRG
DSMFPPGGVAVNTSMGPLIAKIAAEIVKVPGKVTVLGYTDNLPIHSHAFASNDALSAERATQVLQMLQADGVPASRLEAM
GKGEADPVADNSTPQGRAQNRRVEIHVAE*

  Nucleotide sequence: 1290 bp    

>T6CP002766 NC_010625:c1677233-1675944 [Paraburkholderia phymatum STM815] [TssL]
ATGAGCGACAAGGCTGCGCTTTTGAGGGGTACGGCACGCGATACCGAAGCCCAGTCCTATGCTGACGCCGCTGTGTCCGG
CCAGGAAGGGCTGCCCTATGAGGTTCCACTGGGCGAGCCGGCTCTGGAGCGGCTGACCAAAGTCCGCTCGGCTGCAAACC
CGCTGCTCGAAGCTGCGCGTGTGCTGTTGCGGTCGCAGGCCGACATTCCGGAAATGGATCTCGGTGCGAAAGGTGTTATC
CCGACCCTGCGGCAGTTGCTGCTCGATGAGATGAAAACCTTCCAGAAGCTCTGCGAGCAGACCAGCATTCGGCGCGACCA
CATGATTGGCGCCCGCTACTGCCTGTGTACCGCCCTCGATGAAGCGGTCATGGAGACGCCATGGGGCCGTACAGGAGACA
ATGGTGTGTCATGGGGAACAGTGGGCCTCGCGACCAACTTCCACGAGGACCGTGACGGAGGAACCAAGATCTATCTGCTC
GTCGCGCGCTTGATGCAGGATCCGGACCAGCACGACGACCTGCTTGAAGTGATCTACCACATCCTGAGCTTCGGTTTTGA
GGGCCGTTACCGTAGCGAGCGCGACGGTCATCGCAAGCACGAAGCCGTTCGCCAGCGTATCTACAACGAGATCATGGCGC
GGCGGCCGCCGGTGCCGGTGGAACTTTCCCCGCACGCCCGCAGCGACGTGCCGCCCAGGCGCCAGTCGTTCTATGACTTT
CCGGTCTGGATTACGGTTACCGTGCTCTCGCTCATTCTGCTTGGCATGTGGGGCTACTTCAAGTACCAGCTCGTGACACG
TGCCGACGATATCCAGAAGCAGATCGGTGACATCGCACGCATGACGCCGCCACCTGCGCCGCCGGTGCTACATCTGAAAA
CCCTACTCAAGGACGAGATCGCCGCCGGCACCGTGAGCGTGGACGAGGACGCGAAGCACAGCGCGGTGACGTTCCGCGGC
GACTCGATGTTCCCGCCCGGCGGTGTTGCGGTCAACACGTCAATGGGGCCGCTGATCGCAAAGATTGCCGCCGAGATCGT
CAAGGTGCCGGGCAAGGTCACGGTGCTGGGCTACACGGACAACCTCCCGATCCACAGCCACGCATTCGCTTCCAATGATG
CGCTGTCGGCCGAGCGTGCCACGCAGGTGCTGCAGATGCTGCAGGCCGACGGTGTACCTGCCAGCCGTCTCGAAGCCATG
GGCAAGGGCGAAGCTGACCCGGTTGCCGATAACAGCACGCCACAGGGCCGCGCCCAGAACCGTCGGGTCGAGATCCATGT
TGCGGAATGA