SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002763
Component protein type	TssI
T6SS ID (Type)	T6SS00275 (Type i3)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (715 a.a.); Nucleotide sequence (2145 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS30565
Coordinate (Strand)	676639..678783 (+)
NCBI ID	WP_012405340.1
RefSeq	NC_010625
Uniprot ID	B2JW54_PARP8
KEGG ID	bph:Bphy_6124
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	7.8e-88	36..359
Phage_base_V	PF04717.14	6e-15	414..482

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-714	MLQFLTNRTLTIDGPAIPLSSTGDPVLQLQSIAGTEILSRIYRYVLDCVTVSDLLMTDAASLDLKSMIGKELTVTVQLDGMGSFVPGMTGLSGAANIGKGIREISGIVTEARFVGQSNRQGHYRLLLQPWITLADQQSTYRIFQNKTVVEIVDQVFGSYMFSWDKRLSAEYKELEYQVQYGETDFIFAQRLMQEHGIYWFFEHSNKIHRMVLVDQLGAHKPVDSAAYQTLQYYPPGHKIDMEYIDSFDASESIQSGRWTTSDFNFKKPRARLGTENALPRDTEFNELERYEWPGDYSDTAVGEDFARIRMQELYAPGTRAWGSGNLRDVVCGTTFTLAGYPQQSANCEYLVVSASFAASELGESTGAGEFSVRSSFVVQPATVVFRPPRTVPKPRTTGPQTAIVTGPDGQEIWTDQYGRVKLKFHWDRSPVRNQKSSCWVRVAYPWAGNNFGTINIPRIGQEVIVDFENGDPDRPIVTGRVYNGANMPPWPLPDNATQSGTLSRSEDGNYETANAIRFEDKKGREEVWIHAEKDMRTEVENDEVHAVEHDQTITINHDHTKHVGNDEQVDIGHDRTHRITQDDSLAVGRSLTLTVSKDYTGNVGNNRTEKVAADYQIQTGGNVQHTVQGRHQLQAGQGIARKTQVYELVAGERLVLKAPGGSLTIDSSGITLNGISIHLKGAVTQENGTGGAPTLNGIPVPAQLPDPTHLPLSD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9890	-	-

Protein sequence: 715 a.a.

						>T6CP002763 NC_010625:676639-678783 [Paraburkholderia phymatum STM815] [TssI]
MLQFLTNRTLTIDGPAIPLSSTGDPVLQLQSIAGTEILSRIYRYVLDCVTVSDLLMTDAASLDLKSMIGKELTVTVQLDG
MGSFVPGMTGLSGAANIGKGIREISGIVTEARFVGQSNRQGHYRLLLQPWITLADQQSTYRIFQNKTVVEIVDQVFGSYM
FSWDKRLSAEYKELEYQVQYGETDFIFAQRLMQEHGIYWFFEHSNKIHRMVLVDQLGAHKPVDSAAYQTLQYYPPGHKID
MEYIDSFDASESIQSGRWTTSDFNFKKPRARLGTENALPRDTEFNELERYEWPGDYSDTAVGEDFARIRMQELYAPGTRA
WGSGNLRDVVCGTTFTLAGYPQQSANCEYLVVSASFAASELGESTGAGEFSVRSSFVVQPATVVFRPPRTVPKPRTTGPQ
TAIVTGPDGQEIWTDQYGRVKLKFHWDRSPVRNQKSSCWVRVAYPWAGNNFGTINIPRIGQEVIVDFENGDPDRPIVTGR
VYNGANMPPWPLPDNATQSGTLSRSEDGNYETANAIRFEDKKGREEVWIHAEKDMRTEVENDEVHAVEHDQTITINHDHT
KHVGNDEQVDIGHDRTHRITQDDSLAVGRSLTLTVSKDYTGNVGNNRTEKVAADYQIQTGGNVQHTVQGRHQLQAGQGIA
RKTQVYELVAGERLVLKAPGGSLTIDSSGITLNGISIHLKGAVTQENGTGGAPTLNGIPVPAQLPDPTHLPLSD*					

Nucleotide sequence: 2145 bp

						>T6CP002763 NC_010625:676639-678783 [Paraburkholderia phymatum STM815] [TssI]
ATGCTGCAATTTCTGACAAACCGTACATTGACGATTGACGGTCCTGCCATCCCCCTTTCGTCCACTGGCGATCCAGTGCT
GCAACTTCAGTCGATCGCGGGTACAGAAATTCTCTCCAGGATCTACAGGTATGTGCTGGATTGCGTCACTGTCTCTGATC
TGTTGATGACCGATGCTGCCAGCCTCGATCTGAAATCGATGATCGGCAAGGAGTTGACCGTCACGGTGCAGCTGGATGGC
ATGGGTTCCTTCGTGCCGGGAATGACGGGACTGTCTGGTGCCGCCAATATCGGCAAAGGCATCCGGGAGATCAGCGGCAT
TGTTACGGAAGCACGATTTGTCGGTCAGTCAAACCGACAGGGCCACTATCGTCTGCTCCTCCAGCCGTGGATCACGCTTG
CGGATCAGCAATCGACTTACAGAATTTTCCAGAATAAGACGGTCGTCGAAATCGTCGATCAGGTATTTGGTTCCTACATG
TTCTCCTGGGACAAGCGCCTGAGTGCAGAATACAAGGAACTCGAATACCAGGTCCAGTATGGTGAAACGGACTTTATATT
TGCCCAGAGGCTCATGCAGGAGCATGGCATTTACTGGTTCTTTGAGCACTCTAACAAGATCCACCGGATGGTTCTTGTCG
ATCAGCTTGGTGCCCACAAGCCGGTTGATAGTGCAGCATATCAGACTCTGCAGTATTACCCGCCCGGCCACAAGATCGAT
ATGGAATATATTGACAGCTTCGACGCGAGCGAAAGCATTCAGTCGGGTCGGTGGACAACGAGCGACTTCAATTTCAAGAA
GCCGAGGGCACGACTCGGAACCGAAAACGCGTTGCCGCGTGATACCGAGTTCAATGAACTGGAACGCTACGAATGGCCGG
GCGACTATAGCGACACAGCCGTCGGAGAAGACTTCGCGCGGATACGAATGCAGGAGCTTTACGCGCCAGGCACTCGTGCT
TGGGGGAGTGGCAATCTGCGCGACGTGGTTTGTGGCACAACGTTCACGCTCGCAGGCTACCCGCAGCAAAGTGCAAATTG
CGAGTACCTGGTTGTCAGCGCATCGTTTGCAGCTTCGGAGCTGGGTGAATCCACTGGTGCGGGTGAGTTCAGTGTCCGCT
CGTCGTTCGTCGTGCAACCGGCGACCGTCGTTTTCCGTCCTCCGCGCACTGTTCCGAAACCCCGCACGACTGGGCCTCAA
ACCGCCATCGTCACTGGGCCGGATGGACAGGAAATCTGGACTGACCAGTATGGCCGCGTCAAGTTGAAATTCCACTGGGA
TCGCTCGCCCGTGCGCAACCAGAAATCATCCTGCTGGGTACGTGTCGCGTATCCGTGGGCGGGGAACAACTTCGGCACGA
TCAACATTCCCCGCATCGGGCAGGAAGTCATTGTGGACTTCGAGAACGGAGATCCCGATCGGCCGATCGTGACGGGGCGC
GTCTATAACGGCGCGAATATGCCGCCGTGGCCGCTTCCTGATAATGCCACACAAAGCGGGACGCTGTCACGTTCGGAAGA
CGGTAACTACGAGACGGCGAACGCGATCCGCTTTGAGGACAAAAAGGGACGCGAAGAGGTATGGATTCACGCTGAAAAGG
ACATGCGCACCGAGGTGGAAAACGACGAGGTGCACGCGGTGGAACATGACCAGACCATTACGATAAACCACGACCACACA
AAGCACGTGGGCAACGATGAACAGGTCGATATCGGTCACGACCGCACCCACCGCATTACCCAGGACGACTCGCTTGCTGT
CGGCCGCAGTCTCACCCTGACGGTGAGCAAGGACTACACCGGCAACGTTGGTAACAATCGCACCGAGAAAGTCGCCGCCG
ACTACCAGATCCAGACCGGTGGAAACGTTCAGCACACGGTGCAGGGGCGCCACCAGCTTCAGGCTGGACAAGGCATCGCT
CGCAAGACCCAGGTCTATGAACTGGTGGCGGGAGAGCGGCTTGTTCTGAAGGCACCTGGCGGTAGCCTCACCATCGACAG
CTCGGGTATTACCCTGAACGGCATCTCCATCCATCTCAAGGGCGCGGTGACCCAGGAGAACGGAACGGGCGGGGCACCAA
CCCTTAATGGCATTCCGGTGCCAGCGCAGTTGCCTGATCCGACCCATCTGCCGCTGTCTGACTAA					