Detailed information of component protein


Summary

Component ID T6CP002762
Component protein type TssA
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (362 a.a.); Nucleotide sequence (1086 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30555
Coordinate (Strand) 674034..675119 (+)
NCBI ID WP_012405337.1
RefSeq NC_010625
Uniprot ID UPI0000E79532
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 7.5e-30 24..147
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-361 MSNNRKKSQDKPENRGTTHDWMALISTESPCGSDLEYDPEFVVLSASVAAKATAQYGDFVGTPEPVNWSDVDRDCRRLMVRSKDVRLAVLFIRCRTRLAAATGFAEGLGLLAGWLMTFPDLVHPQLGVDEDRSAALEIRMNALQSLTDTDGLISDLREIALTRSTATRLQLRDVERAFAHPRPSDALTPDSVTRQLEELRMQRPSVLSGFDDALESLTAIEGWCREHLSVYAPDLTALKRLLRHVAIPPGSRQTDDVPEEASDAQPDSEESAALAELPTAPDESEASTLSEPSTPRTTGKAYDRHAALNLIREARQWFEQHEPSSPIPVLLKRAEQFVGKRYVDVVKAIPAELLAQWEQEE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9396 - -


  Protein sequence: 362 a.a.    

>T6CP002762 NC_010625:674034-675119 [Paraburkholderia phymatum STM815] [TssA]
MSNNRKKSQDKPENRGTTHDWMALISTESPCGSDLEYDPEFVVLSASVAAKATAQYGDFVGTPEPVNWSDVDRDCRRLMV
RSKDVRLAVLFIRCRTRLAAATGFAEGLGLLAGWLMTFPDLVHPQLGVDEDRSAALEIRMNALQSLTDTDGLISDLREIA
LTRSTATRLQLRDVERAFAHPRPSDALTPDSVTRQLEELRMQRPSVLSGFDDALESLTAIEGWCREHLSVYAPDLTALKR
LLRHVAIPPGSRQTDDVPEEASDAQPDSEESAALAELPTAPDESEASTLSEPSTPRTTGKAYDRHAALNLIREARQWFEQ
HEPSSPIPVLLKRAEQFVGKRYVDVVKAIPAELLAQWEQEE*

  Nucleotide sequence: 1086 bp    

>T6CP002762 NC_010625:674034-675119 [Paraburkholderia phymatum STM815] [TssA]
ATGAGCAATAACCGAAAGAAAAGTCAGGACAAGCCTGAGAACCGCGGCACGACACACGACTGGATGGCGCTGATTTCGAC
CGAATCGCCTTGTGGTAGCGATCTCGAATACGACCCGGAATTCGTCGTGCTGAGTGCAAGCGTTGCCGCAAAAGCGACGG
CTCAGTATGGCGATTTCGTCGGCACGCCGGAGCCGGTGAACTGGAGCGACGTGGACCGCGACTGCCGGCGCCTGATGGTG
CGCAGCAAGGACGTGCGCCTGGCGGTGCTTTTTATCCGGTGTCGCACGCGGCTCGCGGCCGCCACCGGCTTCGCGGAGGG
TCTTGGCCTGCTGGCCGGCTGGTTGATGACCTTCCCTGATCTGGTTCACCCCCAACTGGGCGTGGACGAAGATCGCTCGG
CCGCGCTCGAGATACGCATGAACGCGCTGCAGTCGCTGACGGACACCGATGGGCTGATCTCCGATTTGAGGGAAATCGCG
CTGACCCGGTCGACGGCAACGCGTCTACAACTGCGGGATGTCGAGCGGGCGTTCGCGCACCCGCGGCCGAGTGACGCGCT
GACGCCGGATTCCGTAACCCGGCAGCTCGAAGAACTGCGCATGCAGCGTCCTTCGGTTCTCTCCGGATTTGATGATGCGC
TTGAAAGTCTGACTGCAATCGAAGGCTGGTGTCGCGAGCACCTGAGCGTGTACGCGCCGGACCTCACCGCGCTCAAGCGA
CTCCTTCGTCACGTGGCCATTCCACCAGGCAGCCGCCAGACGGACGACGTCCCCGAGGAGGCGAGTGACGCCCAACCAGA
TAGCGAGGAGAGCGCAGCATTGGCCGAGTTACCGACCGCCCCTGATGAAAGCGAGGCAAGCACGTTGTCCGAACCTTCGA
CACCCCGTACGACCGGCAAGGCGTATGATCGCCACGCTGCGCTCAACCTGATCCGTGAAGCCCGTCAGTGGTTCGAGCAG
CATGAGCCAAGCAGCCCGATTCCGGTATTGCTCAAGCGCGCTGAGCAGTTTGTTGGCAAGCGATACGTCGACGTGGTCAA
GGCAATTCCCGCCGAGCTCCTGGCCCAATGGGAGCAGGAGGAATAA