SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002761
Component protein type	TssG
T6SS ID (Type)	T6SS00275 (Type i3)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (363 a.a.); Nucleotide sequence (1089 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS30550
Coordinate (Strand)	672949..674037 (+)
NCBI ID	WP_012405336.1
RefSeq	NC_010625
Uniprot ID	B2JW50_PARP8
KEGG ID	bph:Bphy_6120
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.3e-94	26..324

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-362	MQRAPFKALIQDIALSQSLLDHLQAEPWRHGFFALMRRIGANPAIDPIGTARLPQAEPFRLGQQASLIFAPREIASVSVDHERLRVLLFGLGMLGPNGPLPIHITEIARERKEHRRDPTLSNFLDIFHHRYLTMLYRVWASAQSAASLDRPGNERFTFYIASLTGHEVKEIEQGPLPAHARLSAAPHLIRESRNPDGLAATLSHYFGVPVKIDEFVFHWLERKPGKRSRLGVPGAATTMAVDGFLGDRIPDRQYRFRIVIGPVDMEDYQRFTPQGSDLLRLVEWVRAFVGQEIHWELELKIRPHSAPPAVVGGPQQLGWSGWLGQSPSPEPVTGMIFEPERYVPQLKQDAETRAARVKQIEP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9798	-	-

Protein sequence: 363 a.a.

						>T6CP002761 NC_010625:672949-674037 [Paraburkholderia phymatum STM815] [TssG]
MQRAPFKALIQDIALSQSLLDHLQAEPWRHGFFALMRRIGANPAIDPIGTARLPQAEPFRLGQQASLIFAPREIASVSVD
HERLRVLLFGLGMLGPNGPLPIHITEIARERKEHRRDPTLSNFLDIFHHRYLTMLYRVWASAQSAASLDRPGNERFTFYI
ASLTGHEVKEIEQGPLPAHARLSAAPHLIRESRNPDGLAATLSHYFGVPVKIDEFVFHWLERKPGKRSRLGVPGAATTMA
VDGFLGDRIPDRQYRFRIVIGPVDMEDYQRFTPQGSDLLRLVEWVRAFVGQEIHWELELKIRPHSAPPAVVGGPQQLGWS
GWLGQSPSPEPVTGMIFEPERYVPQLKQDAETRAARVKQIEP*					

Nucleotide sequence: 1089 bp

						>T6CP002761 NC_010625:672949-674037 [Paraburkholderia phymatum STM815] [TssG]
ATGCAACGTGCTCCGTTCAAGGCGCTGATCCAGGATATCGCGCTGTCGCAATCGCTGCTCGACCACCTGCAGGCCGAGCC
GTGGCGCCATGGCTTTTTTGCGTTGATGCGCCGTATCGGCGCCAACCCGGCGATTGACCCGATCGGTACGGCCAGGCTCC
CGCAGGCTGAGCCCTTCAGGCTCGGCCAGCAGGCGAGCCTCATCTTTGCGCCGCGCGAGATCGCAAGTGTCAGCGTGGAT
CACGAGCGTCTGAGAGTCCTGCTCTTTGGACTGGGCATGCTTGGTCCCAATGGCCCACTGCCGATCCACATCACCGAGAT
TGCACGCGAACGCAAGGAGCATCGACGCGATCCGACGCTCAGCAATTTCCTCGACATCTTCCATCATCGATATCTCACGA
TGCTCTACCGCGTGTGGGCGTCGGCGCAATCCGCAGCGAGTCTGGATCGGCCCGGGAACGAGCGGTTCACGTTCTACATT
GCCAGCCTGACAGGGCACGAGGTCAAGGAGATTGAGCAGGGACCGTTGCCGGCCCACGCGAGGCTCTCCGCCGCGCCACA
CCTGATCCGCGAATCACGCAACCCGGACGGGCTGGCTGCCACGCTCTCCCACTATTTCGGGGTACCGGTCAAGATCGATG
AGTTCGTCTTTCACTGGCTTGAACGCAAGCCCGGCAAACGCAGCAGGCTGGGGGTACCCGGAGCCGCCACGACGATGGCC
GTCGATGGCTTTCTGGGAGACAGGATACCGGATCGTCAGTACCGGTTCCGCATCGTCATTGGCCCCGTTGACATGGAAGA
CTATCAGCGGTTCACGCCGCAGGGCTCCGATCTGCTGCGCCTGGTTGAATGGGTACGCGCGTTTGTTGGTCAGGAAATCC
ACTGGGAACTGGAACTGAAGATTCGCCCGCACAGCGCACCACCGGCTGTCGTGGGCGGTCCCCAGCAGCTGGGCTGGTCC
GGGTGGCTCGGACAGTCTCCGTCACCTGAACCTGTCACCGGGATGATTTTTGAGCCCGAGCGCTACGTTCCCCAGCTCAA
ACAGGATGCCGAAACCCGCGCGGCCAGAGTGAAGCAGATCGAACCATGA					