SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002759
Component protein type	TssE
T6SS ID (Type)	T6SS00275 (Type i3)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (184 a.a.); Nucleotide sequence (552 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS30540
Coordinate (Strand)	670482..671033 (+)
NCBI ID	WP_012405334.1
RefSeq	NC_010625
Uniprot ID	B2JW48_PARP8
KEGG ID	bph:Bphy_6118
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	1.7e-19	53..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-183	MERRRRKADGTQTASTPRRHDTFLMPTLLDRLRDDAPHRQHEAPGEYAVTRAQMRDIVQRDLAYLLNTTNIEEQVDRARYPEAAASTVNFGVPPLAGKFTATRQWADIEIIIKQAITDFEPRLVPGSLSVAPLPDKDGEVQYNALAFEVRGLISMDPYPLEFLVQSSLDLETSQLNVSGARAV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9634	-	-

Protein sequence: 184 a.a.

						>T6CP002759 NC_010625:670482-671033 [Paraburkholderia phymatum STM815] [TssE]
MERRRRKADGTQTASTPRRHDTFLMPTLLDRLRDDAPHRQHEAPGEYAVTRAQMRDIVQRDLAYLLNTTNIEEQVDRARY
PEAAASTVNFGVPPLAGKFTATRQWADIEIIIKQAITDFEPRLVPGSLSVAPLPDKDGEVQYNALAFEVRGLISMDPYPL
EFLVQSSLDLETSQLNVSGARAV*					

Nucleotide sequence: 552 bp

						>T6CP002759 NC_010625:670482-671033 [Paraburkholderia phymatum STM815] [TssE]
ATGGAGCGGCGTAGACGCAAAGCCGACGGCACGCAGACCGCCAGCACGCCCCGGCGGCACGATACTTTTCTAATGCCCAC
GCTGCTGGACCGGCTGCGGGATGACGCACCACACCGGCAGCACGAGGCGCCCGGCGAGTATGCCGTGACGCGCGCGCAGA
TGCGCGACATCGTCCAGCGCGATCTGGCGTACCTGCTCAACACGACCAACATCGAGGAGCAGGTTGATCGGGCACGTTAC
CCCGAGGCCGCAGCATCGACCGTGAATTTCGGGGTGCCGCCGCTGGCCGGTAAGTTCACGGCCACCCGTCAATGGGCCGA
CATCGAAATCATCATCAAACAGGCAATTACGGATTTCGAGCCGCGTCTCGTACCGGGCAGCCTCAGCGTGGCGCCGCTAC
CGGACAAGGATGGCGAAGTTCAGTACAACGCGCTCGCGTTTGAGGTGCGCGGACTGATCAGCATGGATCCCTACCCCCTG
GAATTCCTGGTGCAGAGTTCACTTGACCTGGAGACCAGCCAGCTTAACGTCAGCGGTGCACGCGCCGTCTGA					