SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002758
Component protein type	TssH
T6SS ID (Type)	T6SS00275 (Type i3)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (907 a.a.); Nucleotide sequence (2721 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS30525
Coordinate (Strand)	665804..668524 (-)
NCBI ID	WP_012405331.1
RefSeq	NC_010625
Uniprot ID	B2JW45_PARP8
KEGG ID	bph:Bphy_6115
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	2.9e-37	608..774
AAA_lid_9	PF17871.3	1.6e-27	374..462
ClpB_D2-small	PF10431.11	8.8e-13	783..850
AAA	PF00004.31	1.4e-09	232..363

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-906	MTISRQSLFSKLGVTLFRSAESATAYCKLRGNPYVELVHWLHQLMQQPDSDLHRVLRHCEIDQDTLDRDMARALAGLPAGASSIIDFSHDVDHAIERGWLITSLDRNERRIRGAWLLVALLTTPELRRALLSISPAFAKIPVDGLSDVLPAWIDGSPEMGEAPHDSSDYSPALPGEASQAMPRTSKGSPLEQYCTDLTARARAGEIDPVVGRELEIRTMIDVLLRRRQNNPLLTGEAGVGKTAVVEGFALAVANGEVPPKLASVRLLSLDVGALLAGASMKGEFEARLKGVLEAAAKSQVSVILFVDEIHTLIGAGGQAGTGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTRRFQVLQVPEPEEVAAIDMVRGLAPTFARHHGVVILDEAIRAAVRFSHRYIPSRQLPDKAISLFDTACARVALSQHTPPRELQNLSERLRAATVERDLLQQEALIGLDADQAIASANARIEALTNDMAVVEGHWRAQLDAMQALASAREAALAGDQQAAGSLRDLEQQLTFLRGETPYVFPEVDEAIVAEIVSDWTGVPVGRMVTDEITAVRTLPSTLEARVIGQPDALHQIGERVQTARAGLTNPKKPLGVFLLAGPSGVGKTETALALAEALYGGEQNLITLNMSEYQEAHTVSGLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRYIDFRNTTILLTSNVGSELTASLCADPALAPDTEALRAALAPELLKVFPAAFLGRITVVPYRPLASMSLARIVRLHLERVVRRMAETHQIVLNYEEAVVDYIVGRCLVQETGARVLIGFIEQHVLPRLSTLWLDAFSSKQPLSRIDIVLGDRDAPPATALLFRFPAAQQQASQTDGVNADAEESIVGQAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.6716	-	-

Protein sequence: 907 a.a.

						>T6CP002758 NC_010625:c668524-665804 [Paraburkholderia phymatum STM815] [TssH]
MTISRQSLFSKLGVTLFRSAESATAYCKLRGNPYVELVHWLHQLMQQPDSDLHRVLRHCEIDQDTLDRDMARALAGLPAG
ASSIIDFSHDVDHAIERGWLITSLDRNERRIRGAWLLVALLTTPELRRALLSISPAFAKIPVDGLSDVLPAWIDGSPEMG
EAPHDSSDYSPALPGEASQAMPRTSKGSPLEQYCTDLTARARAGEIDPVVGRELEIRTMIDVLLRRRQNNPLLTGEAGVG
KTAVVEGFALAVANGEVPPKLASVRLLSLDVGALLAGASMKGEFEARLKGVLEAAAKSQVSVILFVDEIHTLIGAGGQAG
TGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTRRFQVLQVPEPEEVAAIDMVRGLAPTFARHHGVVILDEAIR
AAVRFSHRYIPSRQLPDKAISLFDTACARVALSQHTPPRELQNLSERLRAATVERDLLQQEALIGLDADQAIASANARIE
ALTNDMAVVEGHWRAQLDAMQALASAREAALAGDQQAAGSLRDLEQQLTFLRGETPYVFPEVDEAIVAEIVSDWTGVPVG
RMVTDEITAVRTLPSTLEARVIGQPDALHQIGERVQTARAGLTNPKKPLGVFLLAGPSGVGKTETALALAEALYGGEQNL
ITLNMSEYQEAHTVSGLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRYIDF
RNTTILLTSNVGSELTASLCADPALAPDTEALRAALAPELLKVFPAAFLGRITVVPYRPLASMSLARIVRLHLERVVRRM
AETHQIVLNYEEAVVDYIVGRCLVQETGARVLIGFIEQHVLPRLSTLWLDAFSSKQPLSRIDIVLGDRDAPPATALLFRF
PAAQQQASQTDGVNADAEESIVGQAL*					

Nucleotide sequence: 2721 bp

						>T6CP002758 NC_010625:c668524-665804 [Paraburkholderia phymatum STM815] [TssH]
ATGACGATTTCCCGTCAGTCCCTGTTCAGCAAGCTGGGAGTCACGTTGTTCAGGAGTGCTGAATCTGCAACGGCGTACTG
TAAACTCAGAGGCAATCCCTACGTCGAGCTGGTGCACTGGTTGCACCAGCTTATGCAGCAGCCTGATTCTGACCTGCATC
GTGTGCTGCGTCATTGTGAAATTGATCAGGACACGCTGGATCGTGACATGGCCCGTGCACTTGCGGGGTTGCCGGCAGGT
GCCAGTTCCATTATCGATTTTTCGCACGATGTCGATCACGCCATCGAGCGTGGCTGGCTCATCACATCGCTCGACAGGAA
TGAACGGCGTATTCGCGGCGCCTGGTTGCTGGTCGCCCTTCTGACGACGCCCGAACTGCGGCGTGCGCTGCTTTCCATTT
CACCGGCCTTCGCCAAGATTCCCGTAGACGGATTGAGCGATGTGCTGCCGGCGTGGATTGATGGTTCGCCCGAAATGGGC
GAAGCCCCGCACGATAGCAGCGACTACTCCCCCGCCCTTCCCGGCGAAGCCTCCCAGGCCATGCCGCGTACTTCAAAAGG
CTCGCCACTTGAACAGTACTGCACCGACCTGACGGCACGCGCACGTGCTGGCGAGATCGACCCCGTGGTGGGGCGCGAAC
TCGAGATCCGCACGATGATCGATGTGCTGCTGCGTCGCCGCCAGAACAACCCGCTGCTCACGGGTGAAGCCGGCGTTGGC
AAAACCGCTGTTGTAGAAGGGTTCGCACTCGCAGTCGCCAACGGTGAAGTCCCCCCCAAACTCGCCAGTGTGCGCTTGCT
GAGCCTCGACGTCGGTGCCCTCCTTGCCGGCGCCAGCATGAAGGGCGAATTCGAGGCGCGCCTGAAAGGCGTGCTTGAAG
CTGCCGCAAAGTCGCAGGTTTCGGTGATTCTTTTCGTGGATGAAATCCATACGCTGATCGGTGCAGGTGGTCAGGCAGGC
ACGGGCGACGCCGCCAACCTCCTCAAGCCGGCGCTCGCACGCGGTACGATTCGCACCATCGGCGCGACAACGTGGGCCGA
GTACAAGCGGCACATTGAGAAGGATCCTGCGCTCACCCGCCGCTTCCAGGTCCTTCAGGTTCCGGAGCCGGAGGAAGTGG
CCGCCATCGACATGGTGCGCGGTCTCGCGCCAACGTTTGCCCGGCATCATGGTGTCGTCATTCTCGACGAAGCGATTCGT
GCCGCGGTGAGGTTTTCGCATCGCTACATTCCCTCGCGGCAGTTGCCCGACAAGGCGATCAGCCTGTTCGATACGGCCTG
CGCCCGCGTGGCGCTCTCGCAGCATACGCCACCGCGCGAACTGCAAAACCTGTCCGAGCGTCTGCGTGCGGCCACGGTCG
AGCGCGACCTGCTCCAGCAGGAAGCGCTCATCGGTCTGGATGCGGATCAGGCCATCGCCTCTGCGAACGCGCGCATCGAA
GCCCTGACAAACGACATGGCCGTTGTCGAAGGACACTGGCGCGCCCAGCTCGACGCCATGCAGGCGCTTGCGAGCGCGCG
TGAGGCAGCGCTCGCCGGCGATCAGCAGGCAGCAGGTTCCTTGCGTGATCTTGAGCAACAGCTCACATTCCTCCGGGGCG
AGACGCCTTATGTATTCCCGGAAGTCGATGAAGCCATTGTCGCGGAAATCGTGTCGGACTGGACCGGCGTCCCCGTCGGG
CGCATGGTGACTGACGAGATCACGGCCGTTCGCACACTGCCCTCAACGCTCGAAGCGAGGGTCATCGGCCAACCCGATGC
GCTCCACCAGATCGGCGAGCGCGTGCAGACGGCGCGTGCCGGCCTCACGAACCCGAAGAAGCCCCTTGGCGTGTTCCTGC
TCGCGGGCCCTTCCGGTGTCGGCAAGACCGAGACGGCGCTCGCCCTTGCTGAAGCGCTGTATGGCGGCGAGCAGAACCTG
ATCACCCTCAACATGAGCGAGTATCAGGAAGCTCACACGGTCTCGGGGCTCAAGGGCGCGCCACCGGGTTACGTCGGTTA
CGGCGAGGGCGGCGTACTGACCGAAGCCGTGCGACGCCGCCCGTATTCGGTGGTGCTGCTCGACGAGATCGAGAAGGCGC
ATCGCGACGTCCACGAAATGTTCTTCCAGGTGTTCGACAAGGGCTACATGGAAGATGGTGACGGGCGGTATATCGACTTC
CGCAATACCACGATCCTGCTTACCAGCAACGTGGGCTCGGAGCTGACGGCAAGCCTGTGTGCGGACCCGGCGCTTGCACC
GGATACAGAGGCCTTGCGTGCTGCGCTCGCCCCCGAACTGCTCAAGGTGTTCCCGGCCGCATTCCTTGGTCGCATCACCG
TCGTGCCATACCGGCCGCTCGCCAGCATGTCACTCGCGCGCATCGTCAGGCTGCACCTGGAGCGCGTCGTGCGCCGCATG
GCCGAGACACACCAGATCGTACTGAACTACGAGGAGGCTGTCGTCGATTACATCGTCGGTCGATGTCTCGTGCAGGAGAC
GGGTGCACGTGTGCTGATCGGCTTCATCGAGCAGCACGTCCTGCCGCGTCTTTCTACCCTGTGGCTCGATGCCTTTTCGT
CGAAGCAGCCGCTCTCACGCATCGATATTGTCCTGGGCGATCGTGACGCTCCGCCTGCTACCGCGCTGCTGTTCCGGTTC
CCGGCTGCGCAGCAGCAGGCCAGCCAGACCGATGGCGTCAACGCTGACGCCGAGGAATCCATCGTCGGGCAGGCTCTCTG
A					