Detailed information of component protein


Summary

Component ID T6CP002756
Component protein type TssC
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (498 a.a.); Nucleotide sequence (1494 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30515
Coordinate (Strand) 663703..665196 (-)
NCBI ID WP_012405329.1
RefSeq NC_010625
Uniprot ID B2JW43_PARP8
KEGG ID bph:Bphy_6113
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 5.9e-133 66..369
VipB_2 PF18945.2 1.8e-45 379..490
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-497 MAKQHAQTAVAGVATGSDFTQLLNQEFRPKTQQAREAVELAVQTLAEQALRQSATISDDAYKSIAAIIAQIDHKLSEQINLILHHDDYQKLESAWRGLHHLVSNTETDERLKIRFMDISKDELRRTMKRYKGLAWDQSPLFKQIYEEEYGQLGGEPYGCLVADYYFDHTPPDVDLLGSIAKISAAAHTPFISGASPAVLQMESWQELANPRDLTKIFTQNLEYAPWNSLRNTEDARYIGLAMPRFLARLPYGAKTNPVDEFDFEEDTAGSDHRHYAWSNAAYAMGVNINRSFKLYGWCSLIRGVESGGTVENLPCHTFPTDDGGVDMKCPTEIAISDRREAELSKNGFIPLIHRKNTDHATFIGAQSMQKPAEYHDADATANANLSARLPYLFACSRFAHYLKCIVRDKIGAFKEREDMQRWLNEWIMNYVDADPANSSQETKARRPLAAAEVLVEEVEGNPGYYQAKFFLRPHFQLEGLTVSLRLVAKLPSIKEAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9667 - -


  Protein sequence: 498 a.a.    

>T6CP002756 NC_010625:c665196-663703 [Paraburkholderia phymatum STM815] [TssC]
MAKQHAQTAVAGVATGSDFTQLLNQEFRPKTQQAREAVELAVQTLAEQALRQSATISDDAYKSIAAIIAQIDHKLSEQIN
LILHHDDYQKLESAWRGLHHLVSNTETDERLKIRFMDISKDELRRTMKRYKGLAWDQSPLFKQIYEEEYGQLGGEPYGCL
VADYYFDHTPPDVDLLGSIAKISAAAHTPFISGASPAVLQMESWQELANPRDLTKIFTQNLEYAPWNSLRNTEDARYIGL
AMPRFLARLPYGAKTNPVDEFDFEEDTAGSDHRHYAWSNAAYAMGVNINRSFKLYGWCSLIRGVESGGTVENLPCHTFPT
DDGGVDMKCPTEIAISDRREAELSKNGFIPLIHRKNTDHATFIGAQSMQKPAEYHDADATANANLSARLPYLFACSRFAH
YLKCIVRDKIGAFKEREDMQRWLNEWIMNYVDADPANSSQETKARRPLAAAEVLVEEVEGNPGYYQAKFFLRPHFQLEGL
TVSLRLVAKLPSIKEAA*

  Nucleotide sequence: 1494 bp    

>T6CP002756 NC_010625:c665196-663703 [Paraburkholderia phymatum STM815] [TssC]
ATGGCAAAACAGCATGCACAGACAGCGGTGGCCGGCGTCGCAACCGGTTCGGACTTCACCCAGTTGCTCAACCAGGAATT
CCGGCCGAAGACCCAACAGGCACGCGAAGCCGTCGAACTCGCCGTCCAGACGCTCGCCGAACAGGCGCTCCGTCAATCAG
CCACCATCAGCGACGATGCGTACAAGAGCATCGCGGCCATCATTGCACAGATCGATCACAAGCTCTCCGAGCAGATCAAC
CTGATCCTGCACCACGACGATTACCAGAAGCTGGAATCGGCCTGGCGTGGTCTGCATCACCTCGTCTCCAATACGGAAAC
GGATGAGCGCCTGAAGATCCGCTTCATGGACATCTCGAAGGACGAGCTGCGCCGCACGATGAAGCGCTACAAGGGCCTCG
CATGGGACCAGAGCCCGCTCTTCAAGCAGATCTACGAAGAAGAGTACGGCCAGCTCGGTGGCGAGCCGTACGGCTGTCTC
GTCGCGGACTACTACTTCGACCACACACCGCCGGACGTCGACCTGCTCGGCTCGATCGCCAAGATCTCGGCCGCAGCCCA
CACCCCGTTCATCTCGGGCGCGTCGCCCGCCGTGCTGCAGATGGAATCGTGGCAGGAGCTCGCCAATCCGCGCGACCTCA
CGAAGATCTTCACGCAGAACCTCGAATATGCCCCGTGGAACTCGCTACGCAACACCGAGGACGCGCGCTATATCGGTCTT
GCGATGCCGCGCTTCCTGGCGCGCCTGCCGTATGGGGCAAAAACGAACCCGGTCGACGAATTCGACTTCGAGGAAGACAC
CGCGGGCTCGGATCACCGTCACTACGCATGGTCCAACGCGGCCTACGCGATGGGCGTGAACATCAACCGCTCGTTCAAGC
TGTACGGCTGGTGCTCGCTGATCCGCGGCGTGGAATCGGGTGGCACGGTCGAAAACCTGCCCTGCCACACGTTCCCGACG
GACGATGGCGGCGTCGACATGAAGTGCCCGACCGAAATCGCCATCTCGGATCGCCGCGAGGCGGAGCTGTCGAAGAACGG
TTTCATCCCGCTCATTCACCGCAAGAACACCGACCACGCGACCTTCATCGGTGCGCAGTCGATGCAGAAGCCGGCCGAGT
ACCACGATGCTGACGCAACCGCTAACGCGAACCTGTCCGCGCGCCTGCCGTACCTGTTCGCCTGCTCGCGTTTCGCGCAT
TACTTGAAGTGCATCGTGCGAGACAAGATCGGTGCGTTCAAGGAGCGTGAGGACATGCAGCGCTGGCTCAACGAATGGAT
CATGAACTACGTTGACGCCGACCCGGCCAACTCGTCGCAGGAAACCAAGGCCCGTCGTCCGCTCGCCGCAGCCGAGGTGC
TCGTCGAGGAAGTGGAAGGCAACCCTGGCTACTACCAGGCGAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAAGGCCTG
ACCGTGTCACTGCGCTTGGTCGCGAAGCTGCCGTCGATCAAGGAAGCCGCCTGA