Detailed information of component protein


Summary

Component ID T6CP002753
Component protein type TssK
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (449 a.a.); Nucleotide sequence (1347 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30500
Coordinate (Strand) 661145..662491 (-)
NCBI ID WP_012405326.1
RefSeq NC_010625
Uniprot ID B2JW40_PARP8
KEGG ID bph:Bphy_6110
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 2.5e-143 20..446
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-448 MSWHNKVVWNEGLFMRPQLFQQQERYLEHFAHKRAAPLSPFFFGFTHYTLDTEALALGKVIVKSVAGVFPDGTPFDAPGSTPPPAPLTIRPEHLEQLIYLAVPIRVPNGEETTFDNASDSLARYAVFNTDLRDTNSVGQGSQKIQLSNLRLRLVPEKEMTDAWIGLALTRVKTIRADGSIVLDDTLVPPVSAYGASSLLESWLAKIHDLTRLRATALAQRLTGADGKAGTAAEVSDYLLLQTLNRYEPLLQHLRRVPTTSPADLYALLISMAGELSTYVRTTTRRPLDSHPAYQHTAPHVCLKPVVDDTHQLLNAVLVRSAQSIELTDTGYGMRNAVVDPAEMHGFTSVVLGVSAAMPPDTLQQQFAAQAKVGPSERLPDLVRGHLPGITLQALPVPPRQIPFNAGYIYYELSRAGQLWEDVERRGGLALHIAGDFPSLKLELWGIRG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9961 - -


  Protein sequence: 449 a.a.    

>T6CP002753 NC_010625:c662491-661145 [Paraburkholderia phymatum STM815] [TssK]
MSWHNKVVWNEGLFMRPQLFQQQERYLEHFAHKRAAPLSPFFFGFTHYTLDTEALALGKVIVKSVAGVFPDGTPFDAPGS
TPPPAPLTIRPEHLEQLIYLAVPIRVPNGEETTFDNASDSLARYAVFNTDLRDTNSVGQGSQKIQLSNLRLRLVPEKEMT
DAWIGLALTRVKTIRADGSIVLDDTLVPPVSAYGASSLLESWLAKIHDLTRLRATALAQRLTGADGKAGTAAEVSDYLLL
QTLNRYEPLLQHLRRVPTTSPADLYALLISMAGELSTYVRTTTRRPLDSHPAYQHTAPHVCLKPVVDDTHQLLNAVLVRS
AQSIELTDTGYGMRNAVVDPAEMHGFTSVVLGVSAAMPPDTLQQQFAAQAKVGPSERLPDLVRGHLPGITLQALPVPPRQ
IPFNAGYIYYELSRAGQLWEDVERRGGLALHIAGDFPSLKLELWGIRG*

  Nucleotide sequence: 1347 bp    

>T6CP002753 NC_010625:c662491-661145 [Paraburkholderia phymatum STM815] [TssK]
ATGAGCTGGCACAACAAGGTCGTCTGGAATGAGGGGCTCTTCATGCGCCCGCAACTATTCCAGCAGCAGGAGCGTTATCT
CGAGCATTTCGCGCACAAGCGCGCGGCCCCCCTTTCGCCCTTCTTCTTTGGCTTTACGCACTACACCCTCGACACCGAGG
CGCTCGCGCTCGGCAAGGTGATCGTGAAGTCCGTGGCCGGCGTTTTTCCGGATGGCACCCCTTTCGATGCACCAGGCAGC
ACGCCCCCGCCCGCGCCGCTCACGATCCGGCCCGAGCATCTGGAGCAGCTCATCTATCTCGCTGTGCCGATCCGTGTCCC
CAACGGCGAGGAAACGACTTTCGACAACGCGTCGGACTCTCTCGCACGCTACGCTGTGTTCAACACGGACCTGCGCGACA
CCAACTCGGTCGGTCAAGGTTCACAGAAAATCCAGCTGTCGAACCTGCGGCTGCGTCTCGTGCCCGAGAAGGAGATGACC
GATGCGTGGATTGGCCTTGCGCTCACGCGCGTCAAGACCATTCGCGCCGACGGCAGCATTGTGCTCGACGACACCCTCGT
GCCGCCTGTCTCAGCCTACGGCGCAAGCAGCCTGCTCGAGAGCTGGCTTGCGAAAATCCATGACCTCACCCGCCTGCGCG
CCACGGCGCTCGCGCAGCGTCTGACCGGTGCAGACGGCAAGGCCGGCACCGCGGCCGAGGTGTCGGACTACCTGCTGCTG
CAGACACTCAACCGCTACGAACCGCTATTACAGCATCTGCGGCGGGTGCCGACCACCTCGCCTGCGGACCTGTACGCCCT
GCTCATCAGCATGGCGGGCGAGCTGTCAACCTATGTCCGCACCACGACGCGGCGGCCGCTTGATTCGCATCCGGCGTACC
AGCACACGGCGCCCCATGTATGCCTGAAGCCCGTCGTCGACGACACCCACCAGTTGCTCAACGCCGTGCTTGTGCGCAGT
GCGCAGAGCATCGAACTGACGGATACCGGCTACGGCATGCGCAATGCCGTGGTGGATCCGGCGGAAATGCATGGGTTCAC
CTCGGTCGTGCTCGGGGTCAGCGCCGCGATGCCGCCCGATACGCTGCAGCAGCAGTTCGCTGCACAGGCCAAGGTCGGTC
CTTCCGAACGCCTGCCTGATCTCGTGCGGGGTCACCTGCCAGGTATCACGCTGCAGGCATTGCCGGTGCCACCGCGGCAG
ATCCCGTTCAATGCGGGCTACATCTACTACGAACTCTCACGCGCCGGTCAGCTCTGGGAAGACGTCGAACGCCGTGGCGG
TCTGGCACTGCACATCGCGGGGGACTTCCCGTCGCTGAAGCTCGAATTGTGGGGCATTCGCGGGTAA