Detailed information of component protein


Summary

Component ID T6CP002752
Component protein type TssL
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (448 a.a.); Nucleotide sequence (1344 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30495
Coordinate (Strand) 659734..661077 (-)
NCBI ID WP_012405325.1
RefSeq NC_010625
Uniprot ID B2JW39_PARP8
KEGG ID bph:Bphy_6109
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 9.9e-79 45..276
OmpA PF00691.22 2.9e-16 337..435
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-253 MPQATIDPVKQAPDVNEIQATPRRELPHGESLTTRLAAIKAADNPLLEAARPLLRALADMPEYLDHERVEQLRDLLEQEVRAFQKLCEQTSIRRDHMLGTRYCLCTAIDEAAMQTAWGKNSKVPVQPGQSPRGKDAGAAASDINNHDAGVEWVTRGMATIFHEDRQGGDKVYLLIGRLMSDPQEHLDLLEVIYRILSLGFEGRYRFEAGGHRKHEAVRQRIYNEIMARRGIVPVALSPHWQSDVKGRRASFYD
Transmembrane helix 254-276 FPVWITVTLLSVILLGCFGWFKY
Outside 277-447 ELLGRSAEVQKQIADIGHMTPPPAPPVLHLKALLKDEIAAGTVSVDEDAHHSSVTFRGDSMFPPGGASVKATMGPLIAKIAAEIARVPGKVSVLGYTDNVPIRSRQFASNDALSEERATQVMQILQAAGVPASRLEAVGKGDADPVADNSTPQGRAQNRRVEITVAEYTRP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9812 - -


  Protein sequence: 448 a.a.    

>T6CP002752 NC_010625:c661077-659734 [Paraburkholderia phymatum STM815] [TssL]
MPQATIDPVKQAPDVNEIQATPRRELPHGESLTTRLAAIKAADNPLLEAARPLLRALADMPEYLDHERVEQLRDLLEQEV
RAFQKLCEQTSIRRDHMLGTRYCLCTAIDEAAMQTAWGKNSKVPVQPGQSPRGKDAGAAASDINNHDAGVEWVTRGMATI
FHEDRQGGDKVYLLIGRLMSDPQEHLDLLEVIYRILSLGFEGRYRFEAGGHRKHEAVRQRIYNEIMARRGIVPVALSPHW
QSDVKGRRASFYDFPVWITVTLLSVILLGCFGWFKYELLGRSAEVQKQIADIGHMTPPPAPPVLHLKALLKDEIAAGTVS
VDEDAHHSSVTFRGDSMFPPGGASVKATMGPLIAKIAAEIARVPGKVSVLGYTDNVPIRSRQFASNDALSEERATQVMQI
LQAAGVPASRLEAVGKGDADPVADNSTPQGRAQNRRVEITVAEYTRP*

  Nucleotide sequence: 1344 bp    

>T6CP002752 NC_010625:c661077-659734 [Paraburkholderia phymatum STM815] [TssL]
ATGCCACAGGCAACGATAGATCCCGTGAAGCAAGCCCCGGACGTCAACGAAATCCAGGCAACGCCCCGCCGCGAATTGCC
GCACGGCGAGTCTCTCACAACACGTCTTGCGGCCATCAAGGCCGCGGACAATCCGTTGCTTGAAGCCGCGCGTCCCCTGC
TGCGGGCTCTGGCCGACATGCCGGAGTATCTCGATCATGAGCGGGTCGAGCAACTGCGCGACCTGCTGGAGCAGGAGGTA
CGTGCCTTCCAGAAGCTGTGCGAGCAGACCAGCATCCGGCGGGACCACATGCTCGGCACCCGCTACTGCCTATGTACGGC
CATCGACGAGGCGGCCATGCAGACCGCGTGGGGCAAGAACAGCAAGGTGCCCGTACAGCCAGGACAGAGTCCGCGCGGAA
AGGATGCGGGTGCGGCAGCCTCGGATATCAACAACCACGACGCGGGCGTCGAATGGGTAACCCGTGGGATGGCCACGATC
TTCCATGAAGATCGGCAAGGCGGCGACAAGGTGTACCTCCTCATCGGCCGGCTGATGTCCGATCCGCAGGAGCATCTTGA
CCTGCTCGAAGTGATCTATCGCATCCTGAGTCTGGGCTTCGAAGGCCGCTACCGCTTTGAAGCGGGTGGCCACCGCAAGC
ACGAAGCGGTGCGTCAGCGCATCTACAACGAAATCATGGCGAGACGCGGCATCGTGCCCGTCGCCCTGTCGCCGCACTGG
CAGTCCGACGTCAAGGGCCGGCGTGCGTCGTTCTACGACTTTCCGGTATGGATCACGGTCACGCTGCTTTCGGTCATCCT
GCTCGGCTGCTTTGGGTGGTTCAAGTACGAGCTGCTCGGTCGCAGCGCTGAGGTCCAGAAGCAGATTGCCGACATTGGAC
ACATGACGCCACCGCCGGCGCCTCCAGTCCTGCACCTGAAGGCGTTGCTCAAGGACGAAATTGCCGCGGGCACGGTCAGT
GTCGACGAAGACGCGCATCACAGTTCGGTGACGTTCCGCGGCGATTCGATGTTCCCGCCAGGTGGTGCTTCCGTGAAGGC
AACGATGGGCCCGCTCATCGCGAAGATTGCTGCCGAGATTGCCAGGGTACCCGGCAAGGTTTCGGTGCTGGGCTATACGG
ACAATGTGCCGATCAGAAGCCGCCAGTTCGCATCGAATGACGCACTGTCCGAAGAGCGCGCTACGCAGGTCATGCAGATT
CTGCAGGCTGCCGGCGTGCCGGCAAGCCGCCTGGAGGCCGTTGGCAAGGGCGACGCCGATCCGGTCGCTGATAACAGCAC
GCCCCAGGGCCGCGCACAGAATCGACGTGTCGAAATCACGGTCGCGGAATATACGAGACCATAA