Detailed information of component protein


Summary

Component ID T6CP002751
Component protein type TssM
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (1349 a.a.); Nucleotide sequence (4047 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30490
Coordinate (Strand) 655657..659703 (-)
NCBI ID WP_012405324.1
RefSeq NC_010625
Uniprot ID B2JW38_PARP8
KEGG ID bph:Bphy_6108
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.6e-83 309..572
IcmF-related PF06761.14 3.3e-79 624..945
IcmF_C PF06744.14 7.1e-24 1196..1299
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MKKFLSFLVSRQ
Transmembrane helix 13-35 FLAFLALIVVALVIWYVGPFVAF
Outside 36-44 GGLKPLAGA
Transmembrane helix 45-63 GMRVLVIALLLAGVLLWLV
Inside 64-69 RWSTSP
Transmembrane helix 70-89 VIVALLCLLIWYATPLLTFG
Outside 90-98 KAQPFAPES
Transmembrane helix 99-117 ARIIAIAVALFIFAVYWLF
Inside 118-566 RLWQEMKSNRSLVDRMLTFGGKKEPSPAAVQLKEVGVIVSSALARLKAMRTGARGIGRLFQGKRYLYELPWYIALGSKSSGKTSALLNAGLAFPVAEQMQRAAGAAQKPTANVDWWLTNDAVLIDTAGYYTRHGTSGHALPPPPESNNPTERKATDTRTENEKSGHGKALDAPKASTASTTWADPAQARQRMDSEEWLGFLGMLRTHRPRAPINGALLAVNLGELTSQDDSSRMAEAAALRARLAELRGELGIRFPVYLVVTGMDQLTGFAEYFSSLTAEGRAQPWGFTLPYGKETIAKEGVTARCRSELGQLAQRLERGIDTRLQDEYDPLHRRKLAALSQEFAALSAPLIELIERVFLDSRYDDTQLYSTLRGVYFTSAEQAGNERVAERRTIVQRVIAGMKAPATSLREATSEGFFLHDLFTKVVIPEAHLVRPNLRWEFRFRLLR
Transmembrane helix 567-589 LVGHALALLLFVWLAIGLCVSFG
Outside 590-1348 NNSDYLGAIARKTQALAARVAQLYHDPKAEAVPDTLSAARYLPTYQGLDLSDPDSTFRYGLYTAPDIVTESRHIYDALEDNLLVPQIVHRMEDVISQSITNKDPKATYEALRVYLMLYDKAKFNADDVKAWVLDDWAKTDSASVFGGRASMIDHVQQLFSGTRVVQSPLIRNDALIQQARAFLDGSNATDRLYERAKAAMMKEAPDEFTLIRAVGPQAGTVFTRASGAPLSRGVPGLFTFDGYRNLFDKHLPEFVQVARDDDAWVMGRSYLGGLSDQAQKKTAELVRMATGADDPLTEAIRREYLTEYAQQWDAFLGDIRTVRGTSLAFNLQVLRSFAAPDSPLARLARAAVHETTLTQPVAATGGSLLQKATDQISQKADSALGIRASEQVERELVDSRFAALREVVTGNAEGQATGDAAAQPGKTGLDGVTNLLNDYYTALTVADNAISNNSMPPASDTAAKLKMTANTMPPPFRAVLLQLAQQGSQEVNQGIGQLLSRQMGAVIGDTCRLTIEGNYPFTPGSTRDVSIDDFTRVFAQGGVIDDFFNKTLAPFVDTSAKPWRYRTLPGATEPVQGPDLEPFQHVKAIRDVFFSGPGQNQMTWKANLQVPELDPAITGLMIDIDGQTMLYQHGPVTPFTVSWPGPRGGVHAEITANPRIRPETSTMSADGPWALMRLLAKGHVVETATPGKTLVAFDFDGRRAVLDMDSTGSVANPLTSDVLRTFRCPSSMPVFGLVDSGPPPGLPAAGLPMTPPSPY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9974 - -


  Protein sequence: 1349 a.a.    

>T6CP002751 NC_010625:c659703-655657 [Paraburkholderia phymatum STM815] [TssM]
MKKFLSFLVSRQFLAFLALIVVALVIWYVGPFVAFGGLKPLAGAGMRVLVIALLLAGVLLWLVRWSTSPVIVALLCLLIW
YATPLLTFGKAQPFAPESARIIAIAVALFIFAVYWLFRLWQEMKSNRSLVDRMLTFGGKKEPSPAAVQLKEVGVIVSSAL
ARLKAMRTGARGIGRLFQGKRYLYELPWYIALGSKSSGKTSALLNAGLAFPVAEQMQRAAGAAQKPTANVDWWLTNDAVL
IDTAGYYTRHGTSGHALPPPPESNNPTERKATDTRTENEKSGHGKALDAPKASTASTTWADPAQARQRMDSEEWLGFLGM
LRTHRPRAPINGALLAVNLGELTSQDDSSRMAEAAALRARLAELRGELGIRFPVYLVVTGMDQLTGFAEYFSSLTAEGRA
QPWGFTLPYGKETIAKEGVTARCRSELGQLAQRLERGIDTRLQDEYDPLHRRKLAALSQEFAALSAPLIELIERVFLDSR
YDDTQLYSTLRGVYFTSAEQAGNERVAERRTIVQRVIAGMKAPATSLREATSEGFFLHDLFTKVVIPEAHLVRPNLRWEF
RFRLLRLVGHALALLLFVWLAIGLCVSFGNNSDYLGAIARKTQALAARVAQLYHDPKAEAVPDTLSAARYLPTYQGLDLS
DPDSTFRYGLYTAPDIVTESRHIYDALEDNLLVPQIVHRMEDVISQSITNKDPKATYEALRVYLMLYDKAKFNADDVKAW
VLDDWAKTDSASVFGGRASMIDHVQQLFSGTRVVQSPLIRNDALIQQARAFLDGSNATDRLYERAKAAMMKEAPDEFTLI
RAVGPQAGTVFTRASGAPLSRGVPGLFTFDGYRNLFDKHLPEFVQVARDDDAWVMGRSYLGGLSDQAQKKTAELVRMATG
ADDPLTEAIRREYLTEYAQQWDAFLGDIRTVRGTSLAFNLQVLRSFAAPDSPLARLARAAVHETTLTQPVAATGGSLLQK
ATDQISQKADSALGIRASEQVERELVDSRFAALREVVTGNAEGQATGDAAAQPGKTGLDGVTNLLNDYYTALTVADNAIS
NNSMPPASDTAAKLKMTANTMPPPFRAVLLQLAQQGSQEVNQGIGQLLSRQMGAVIGDTCRLTIEGNYPFTPGSTRDVSI
DDFTRVFAQGGVIDDFFNKTLAPFVDTSAKPWRYRTLPGATEPVQGPDLEPFQHVKAIRDVFFSGPGQNQMTWKANLQVP
ELDPAITGLMIDIDGQTMLYQHGPVTPFTVSWPGPRGGVHAEITANPRIRPETSTMSADGPWALMRLLAKGHVVETATPG
KTLVAFDFDGRRAVLDMDSTGSVANPLTSDVLRTFRCPSSMPVFGLVDSGPPPGLPAAGLPMTPPSPY*

  Nucleotide sequence: 4047 bp    

>T6CP002751 NC_010625:c659703-655657 [Paraburkholderia phymatum STM815] [TssM]
ATGAAAAAATTCCTGTCGTTTCTGGTCTCGCGCCAGTTCCTCGCCTTTCTCGCGCTGATTGTTGTCGCGCTGGTGATCTG
GTATGTCGGACCCTTTGTCGCATTCGGCGGCCTGAAGCCGCTCGCAGGCGCAGGCATGCGTGTGCTCGTCATCGCCCTGC
TGCTCGCGGGCGTTCTGCTCTGGCTGGTCCGCTGGTCGACCAGCCCCGTCATCGTGGCGTTGCTGTGCCTGCTGATCTGG
TACGCGACGCCGCTGCTCACGTTTGGCAAGGCCCAGCCCTTCGCGCCGGAGTCGGCACGCATCATCGCCATCGCGGTCGC
GCTGTTCATTTTCGCCGTGTACTGGCTCTTCCGCCTCTGGCAGGAGATGAAGAGCAACCGCTCGCTGGTCGACCGGATGC
TGACGTTCGGTGGCAAGAAGGAACCGTCGCCTGCCGCAGTACAGCTGAAAGAGGTCGGCGTCATTGTGTCAAGCGCCCTC
GCCCGCCTGAAGGCGATGCGAACCGGCGCGCGTGGCATAGGACGTCTTTTCCAGGGCAAACGCTACCTGTATGAATTGCC
GTGGTATATCGCGCTCGGCTCGAAGTCTTCGGGCAAGACAAGCGCCCTGCTGAACGCGGGTCTCGCGTTCCCTGTTGCCG
AACAGATGCAGCGTGCCGCAGGCGCCGCGCAGAAGCCAACTGCCAACGTCGACTGGTGGCTCACCAACGATGCGGTGCTG
ATCGACACGGCTGGCTACTACACCCGTCACGGCACATCCGGACACGCTTTGCCGCCGCCTCCGGAAAGCAACAATCCCAC
GGAGCGCAAGGCTACCGACACAAGGACGGAGAACGAAAAATCTGGGCATGGGAAAGCCCTCGACGCGCCGAAAGCATCGA
CCGCGTCAACCACGTGGGCCGATCCCGCGCAGGCTCGCCAGCGAATGGACTCGGAGGAATGGCTCGGCTTCCTCGGCATG
CTTCGGACGCACCGGCCGCGCGCACCCATCAACGGTGCGCTGCTCGCGGTCAACCTCGGCGAACTGACGAGCCAGGACGA
CAGCAGCCGCATGGCCGAAGCGGCCGCGTTGCGTGCGCGCCTGGCCGAACTGCGTGGCGAGCTCGGTATCCGCTTTCCGG
TCTATCTGGTCGTGACCGGCATGGACCAGCTCACCGGCTTCGCCGAATACTTTTCGTCGCTAACGGCAGAGGGCCGTGCC
CAGCCATGGGGTTTTACGCTTCCCTACGGCAAGGAAACCATCGCGAAAGAAGGCGTCACCGCACGGTGCCGCTCGGAGCT
GGGCCAGCTCGCACAACGCCTCGAACGTGGCATCGATACCCGCCTGCAGGACGAATACGATCCGCTCCACCGTCGCAAGC
TCGCCGCGCTGTCACAGGAGTTTGCCGCCCTGAGCGCGCCGCTCATCGAACTGATCGAGCGGGTGTTTCTCGATTCACGC
TACGATGACACGCAGCTGTACTCGACGCTGCGCGGCGTGTACTTCACGAGCGCAGAACAGGCTGGCAACGAGAGGGTGGC
CGAGCGGCGCACCATCGTGCAACGGGTGATCGCGGGCATGAAGGCGCCGGCAACATCGCTGCGCGAGGCAACCAGCGAAG
GCTTCTTCCTGCACGACCTCTTCACGAAGGTTGTCATTCCCGAGGCGCATCTCGTGCGCCCGAACCTGCGCTGGGAGTTC
CGCTTCCGTCTGCTACGCCTGGTTGGCCACGCGCTCGCCCTGCTGCTCTTCGTCTGGCTCGCCATTGGACTGTGCGTGAG
CTTCGGCAACAACAGCGATTACCTTGGTGCCATTGCGCGCAAGACGCAGGCGCTCGCGGCGCGTGTGGCGCAGCTGTACC
ACGACCCGAAGGCGGAAGCCGTGCCCGATACGCTGAGCGCCGCGCGCTATCTGCCGACGTATCAGGGGCTCGACCTGTCG
GACCCCGACAGCACCTTCCGCTACGGTCTCTACACGGCCCCCGATATCGTCACGGAAAGCCGCCACATCTATGACGCGCT
TGAGGACAACCTGCTGGTACCGCAGATCGTGCACCGGATGGAAGACGTGATCTCGCAGTCGATCACGAACAAGGATCCGA
AGGCGACTTACGAGGCACTGCGCGTCTATCTCATGCTTTATGACAAGGCGAAATTCAACGCCGATGACGTCAAGGCGTGG
GTACTCGACGACTGGGCGAAGACCGACAGCGCATCCGTATTCGGCGGTCGCGCCTCGATGATCGATCACGTCCAGCAATT
GTTCTCCGGCACCCGGGTAGTCCAGTCGCCGCTCATCCGCAACGACGCTCTGATCCAGCAGGCCCGGGCCTTCCTCGACG
GCAGCAATGCGACCGACCGGCTCTACGAGCGGGCCAAGGCCGCCATGATGAAGGAGGCGCCTGACGAGTTCACGCTGATC
CGCGCGGTGGGCCCCCAGGCGGGGACTGTCTTCACGCGTGCAAGCGGTGCGCCCCTCTCGCGCGGCGTGCCGGGCCTTTT
CACGTTCGACGGCTACCGCAATCTTTTCGACAAACACCTGCCCGAGTTCGTCCAGGTCGCGCGCGATGACGATGCGTGGG
TGATGGGGCGCTCTTATCTCGGGGGGCTCTCCGACCAGGCTCAAAAAAAAACTGCGGAGCTTGTAAGGATGGCCACTGGT
GCCGACGATCCACTGACCGAAGCGATCCGGCGGGAGTACCTCACGGAGTATGCCCAGCAATGGGACGCATTTCTTGGCGA
CATCCGCACGGTGAGAGGGACGAGTCTTGCGTTCAACCTGCAGGTGCTGCGCAGTTTTGCTGCACCTGATTCCCCTCTCG
CGCGGCTAGCGCGCGCGGCCGTGCACGAAACGACGCTCACGCAGCCAGTCGCCGCAACCGGTGGATCACTGCTCCAGAAG
GCGACCGACCAGATCAGCCAGAAGGCTGACTCCGCGCTGGGCATCCGTGCGTCGGAACAGGTCGAGCGCGAACTGGTCGA
CAGCCGTTTCGCTGCGCTGCGTGAGGTCGTGACCGGCAACGCAGAGGGTCAGGCCACGGGCGACGCGGCCGCACAGCCCG
GGAAAACCGGGCTGGACGGTGTGACCAACCTGCTGAACGACTACTACACTGCGCTCACGGTGGCGGATAACGCCATCAGC
AACAACAGCATGCCGCCGGCGAGTGACACGGCCGCGAAGCTGAAAATGACGGCGAACACCATGCCGCCTCCCTTCCGTGC
CGTGCTGCTGCAACTCGCCCAGCAGGGGTCGCAGGAGGTCAATCAGGGCATCGGTCAGCTGCTTTCACGTCAGATGGGTG
CGGTGATCGGCGATACGTGCCGCCTTACGATCGAGGGCAACTACCCGTTCACACCAGGTAGCACGCGCGACGTGAGCATT
GACGACTTTACGCGCGTGTTCGCGCAGGGCGGCGTCATTGACGACTTCTTCAACAAAACGCTGGCCCCCTTCGTCGACAC
CTCCGCGAAACCGTGGCGCTACCGCACGTTGCCCGGTGCGACCGAGCCGGTCCAGGGGCCGGACCTTGAGCCGTTCCAGC
ATGTGAAAGCGATCCGCGATGTCTTCTTCAGCGGCCCGGGCCAGAACCAGATGACGTGGAAAGCTAATCTCCAGGTGCCG
GAGCTCGATCCCGCCATTACCGGCCTCATGATCGATATCGACGGACAGACGATGCTTTACCAGCACGGACCGGTCACGCC
TTTCACGGTTAGCTGGCCCGGCCCTCGCGGCGGCGTACACGCCGAGATCACGGCGAACCCGCGCATCCGGCCCGAGACGT
CAACCATGTCTGCCGATGGCCCATGGGCGTTGATGCGCCTGCTGGCCAAGGGGCACGTCGTGGAGACGGCCACACCCGGA
AAGACACTCGTCGCCTTCGATTTCGATGGGCGCCGCGCCGTGCTCGATATGGACAGTACCGGTAGCGTTGCCAACCCGCT
GACGAGCGACGTCCTGCGAACTTTCCGCTGCCCAAGCTCGATGCCGGTATTCGGTCTTGTCGACAGCGGACCGCCGCCGG
GCCTGCCTGCTGCGGGGCTTCCCATGACGCCACCCAGCCCGTATTGA