Detailed information of component protein


Summary

Component ID T6CP002750
Component protein type TssL
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (411 a.a.); Nucleotide sequence (1233 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30485
Coordinate (Strand) 654398..655630 (-)
NCBI ID WP_012405323.1
RefSeq NC_010625
Uniprot ID B2JW37_PARP8
KEGG ID bph:Bphy_6107
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.3e-42 33..250
OmpA PF00691.22 2.1e-13 305..402
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-230 MSATAASPQPRYEAEPAAPYRERVRAAEGAANPLLEAARPLLDALANTPAELDAAGVAQHRLSLVQHLRMFGKVCGELRLPAEHIEKAQYCLCSALDEAAGLTDWGNGSRLGMDWRATGLATTSGYDRQGGDRVYAIATDAMHNQRENRCLIEVIQHILDRGFRGRYRFANDSDHQIAVVRKQVDQAVVTSGHSPPVSGEHMLTTGGAPPVYHLRVEAQPLSQAPRKSRR
Transmembrane helix 231-253 WMAIGALCLVLLAGAAVAGYWRY
Inside 254-410 TKVRQAEPPVSPIDALAGRLAGHLADEVNAGTVTLAENAQHTTLSLRLEGIFEAGQSSVNPWVKPLITTIGQEVAKTPGRVLVTGHTDSQPFSHMQRSSNLALSDARAQEVAQILVSAGVATDRIDSTGKGNAEPAADNATPQGRLRNRRVEITVFE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9237 - -


  Protein sequence: 411 a.a.    

>T6CP002750 NC_010625:c655630-654398 [Paraburkholderia phymatum STM815] [TssL]
MSATAASPQPRYEAEPAAPYRERVRAAEGAANPLLEAARPLLDALANTPAELDAAGVAQHRLSLVQHLRMFGKVCGELRL
PAEHIEKAQYCLCSALDEAAGLTDWGNGSRLGMDWRATGLATTSGYDRQGGDRVYAIATDAMHNQRENRCLIEVIQHILD
RGFRGRYRFANDSDHQIAVVRKQVDQAVVTSGHSPPVSGEHMLTTGGAPPVYHLRVEAQPLSQAPRKSRRWMAIGALCLV
LLAGAAVAGYWRYTKVRQAEPPVSPIDALAGRLAGHLADEVNAGTVTLAENAQHTTLSLRLEGIFEAGQSSVNPWVKPLI
TTIGQEVAKTPGRVLVTGHTDSQPFSHMQRSSNLALSDARAQEVAQILVSAGVATDRIDSTGKGNAEPAADNATPQGRLR
NRRVEITVFE*

  Nucleotide sequence: 1233 bp    

>T6CP002750 NC_010625:c655630-654398 [Paraburkholderia phymatum STM815] [TssL]
ATGAGTGCGACAGCAGCTTCACCACAACCGCGATACGAAGCCGAACCTGCTGCGCCGTATCGCGAACGCGTTCGCGCGGC
CGAAGGCGCAGCCAATCCCCTGCTCGAAGCTGCTCGCCCACTGCTGGATGCACTCGCGAATACGCCCGCCGAACTCGATG
CAGCCGGCGTTGCCCAGCATCGGCTGTCACTGGTACAGCACCTCCGGATGTTCGGGAAAGTCTGCGGCGAACTCAGGCTG
CCTGCGGAACACATCGAAAAGGCACAATACTGCCTGTGCTCCGCATTGGACGAAGCGGCCGGGCTGACCGACTGGGGCAA
TGGCTCAAGGCTTGGCATGGACTGGCGTGCAACGGGACTCGCCACCACATCGGGCTATGACCGGCAAGGTGGCGACCGCG
TCTACGCCATCGCCACGGATGCCATGCACAATCAGCGTGAAAATCGCTGCCTCATTGAAGTCATCCAGCACATCCTCGAC
CGGGGATTCAGGGGCCGCTACCGGTTCGCCAACGACAGCGACCACCAGATAGCGGTAGTGCGCAAACAGGTCGATCAGGC
CGTCGTCACCTCCGGACACTCTCCTCCAGTGTCCGGTGAACACATGCTGACAACGGGGGGCGCGCCCCCGGTCTATCACC
TGCGGGTCGAGGCGCAGCCGCTATCGCAGGCTCCGCGGAAATCGCGGCGCTGGATGGCTATCGGTGCGTTATGCCTCGTT
CTTCTCGCTGGCGCCGCCGTAGCAGGGTACTGGCGTTACACGAAGGTCAGGCAGGCCGAGCCCCCTGTGTCGCCAATCGA
TGCACTGGCAGGCAGGCTCGCCGGCCACCTCGCGGACGAAGTGAATGCGGGCACCGTCACCCTGGCAGAAAATGCCCAGC
ACACCACGCTCTCCCTCCGGCTCGAGGGCATCTTCGAGGCCGGACAGTCGAGTGTCAATCCGTGGGTCAAGCCCTTGATC
ACCACCATCGGGCAGGAAGTCGCGAAAACTCCCGGAAGGGTCCTCGTCACGGGCCATACCGACAGCCAGCCTTTCTCCCA
TATGCAACGCAGCTCCAACCTGGCACTGTCCGATGCGCGCGCACAGGAGGTTGCACAGATTCTGGTCTCCGCCGGCGTGG
CTACCGATCGCATCGACTCGACCGGAAAGGGTAACGCAGAGCCGGCTGCCGACAACGCGACACCTCAGGGCAGACTGAGA
AATCGCCGTGTCGAGATCACCGTATTCGAGTAG