SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002561
Component protein type	TssM
T6SS ID (Type)	T6SS00259 (Type i2)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (1141 a.a.); Nucleotide sequence (3423 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS12960
Coordinate (Strand)	2847223..2850645 (-)
NCBI ID	WP_012304275.1
RefSeq	NC_010465
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	2e-62	418..735
IcmF_C	PF06744.14	2.4e-22	999..1098

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	VKRIALPIKKPE
Transmembrane helix	13-35	VWFWIVALLFLLAGAVLCWLVWQ
Outside	36-49	HPERVGLIPGTPQR
Transmembrane helix	50-72	DRWLTGLVVGTGILTLCALLSYA
Inside	73-369	GTRLSGRKHFDELRQQAQGDDAPLPEDNAQAGETSQGEPSRLKTRLRRRYGLFWRDKVRLLMVVGEPDEIAALAPQLAEQGWLEGQCTVLIHGGSLQRPADETGLSEWRKLRRGRPLDGIVWAMTAAQSGTPQWMDNGLRTLEKMGAALRYQPPVYLWQVCDSDWPQDSRTEQPVGAVFPAKATPEKVEQQLRALLPQLREQGMQQLSIEPHHDFLLRLAQSLDQGDAARWRQRLTPWFTEYAARIPLRGLMFSLPDTSASAAQVHEKSWTVPASWQGVLDDCRVARGRRVGLPWEQ
Transmembrane helix	370-392	TLCYSLLALIVLWGVGSVVSFTV
Outside	393-1140	NRHQMVSAAQQAQQLAQSHAVSDQQLMALQALRNDIGRLQARVAQGAPWYQRFGLDHNAPLLEVLMPWYGQANNRILRDATAQSLYQKLSELAELPANSPQRAARAKTGYDQLKAYLMMARPEKADAVFYAQVMQTTEPTRAGVSPGLWQSLAPDLWQFYAQNLPAQPDWKITADTGLISQVRQVLLGQMGQRNAESTLYENMLLSVRRNYADMTLMDMTGDTDAQRLFQTSESVPGMFTRKAWDEQIQQAIDKTVASRREEIDWVLSDNRRAISEDISPEALKKRLTERYFTDFAGSWLSFLNSLHWNEAHNLSDVIDQLTLMSDVRQSPLIALMNTLAWQGQTGQQNQALSDSLVKSAKALMNKDQAPAIDQSAGGPVGPLDETFGPLLALMGKGDAQNRLSSDSSLSLQTLLTRVTRVRLKLQQVVNASDPQEMTQVLAQTVFQGKSVDLTDTQEYGSLIAASLGEEWSSFGQTMFVQPLTQAWETVLQPSSASLNDQWKNAVVANWKSAFDGRYPFAASKSDASLPMLAEFIRKDSGRIDSFLTRELGGVLHKEGTRWVPNKVNSQGLTFNPAFLAAINQLSQISDILFTDGSQGLRFELLARPVPNVVETNLAIDGQKLHYFNQMESWQSFRWPGETYKPGTMLTWTGVSSGARLYGDYQGTWGLIRWLEQAKQKKLDEGRYQLTFTTADKQPLQWILRTELGKGPLGLLQLRNFTLPAQIFLIQHAPLAIADMSDDEDMAED

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9947	-	-

Protein sequence: 1141 a.a.

						>T6CP002561 NC_010465:c2850645-2847223 [Yersinia pseudotuberculosis YPIII] [TssM]
VKRIALPIKKPEVWFWIVALLFLLAGAVLCWLVWQHPERVGLIPGTPQRDRWLTGLVVGTGILTLCALLSYAGTRLSGRK
HFDELRQQAQGDDAPLPEDNAQAGETSQGEPSRLKTRLRRRYGLFWRDKVRLLMVVGEPDEIAALAPQLAEQGWLEGQCT
VLIHGGSLQRPADETGLSEWRKLRRGRPLDGIVWAMTAAQSGTPQWMDNGLRTLEKMGAALRYQPPVYLWQVCDSDWPQD
SRTEQPVGAVFPAKATPEKVEQQLRALLPQLREQGMQQLSIEPHHDFLLRLAQSLDQGDAARWRQRLTPWFTEYAARIPL
RGLMFSLPDTSASAAQVHEKSWTVPASWQGVLDDCRVARGRRVGLPWEQTLCYSLLALIVLWGVGSVVSFTVNRHQMVSA
AQQAQQLAQSHAVSDQQLMALQALRNDIGRLQARVAQGAPWYQRFGLDHNAPLLEVLMPWYGQANNRILRDATAQSLYQK
LSELAELPANSPQRAARAKTGYDQLKAYLMMARPEKADAVFYAQVMQTTEPTRAGVSPGLWQSLAPDLWQFYAQNLPAQP
DWKITADTGLISQVRQVLLGQMGQRNAESTLYENMLLSVRRNYADMTLMDMTGDTDAQRLFQTSESVPGMFTRKAWDEQI
QQAIDKTVASRREEIDWVLSDNRRAISEDISPEALKKRLTERYFTDFAGSWLSFLNSLHWNEAHNLSDVIDQLTLMSDVR
QSPLIALMNTLAWQGQTGQQNQALSDSLVKSAKALMNKDQAPAIDQSAGGPVGPLDETFGPLLALMGKGDAQNRLSSDSS
LSLQTLLTRVTRVRLKLQQVVNASDPQEMTQVLAQTVFQGKSVDLTDTQEYGSLIAASLGEEWSSFGQTMFVQPLTQAWE
TVLQPSSASLNDQWKNAVVANWKSAFDGRYPFAASKSDASLPMLAEFIRKDSGRIDSFLTRELGGVLHKEGTRWVPNKVN
SQGLTFNPAFLAAINQLSQISDILFTDGSQGLRFELLARPVPNVVETNLAIDGQKLHYFNQMESWQSFRWPGETYKPGTM
LTWTGVSSGARLYGDYQGTWGLIRWLEQAKQKKLDEGRYQLTFTTADKQPLQWILRTELGKGPLGLLQLRNFTLPAQIFL
IQHAPLAIADMSDDEDMAED*					

Nucleotide sequence: 3423 bp

						>T6CP002561 NC_010465:c2850645-2847223 [Yersinia pseudotuberculosis YPIII] [TssM]
GTGAAGCGCATTGCCTTACCGATTAAAAAGCCAGAAGTCTGGTTCTGGATTGTTGCCCTGCTGTTTTTGCTGGCCGGTGC
CGTGTTGTGCTGGCTGGTCTGGCAACACCCCGAACGGGTGGGGTTAATTCCGGGGACACCGCAACGTGACCGTTGGCTGA
CGGGACTGGTGGTGGGAACGGGGATCCTGACCCTGTGCGCATTACTGTCGTATGCGGGGACCCGGTTATCCGGCAGAAAA
CACTTTGATGAGCTACGGCAGCAGGCACAGGGCGATGATGCGCCGTTGCCGGAAGACAACGCGCAGGCGGGAGAAACGTC
GCAGGGTGAACCGTCCCGCCTAAAAACCCGCCTGCGCCGCCGTTACGGTCTGTTCTGGCGCGACAAAGTCCGGCTGCTGA
TGGTGGTGGGTGAACCTGACGAAATCGCCGCGCTGGCCCCGCAACTGGCGGAGCAGGGCTGGCTGGAAGGGCAGTGCACG
GTGCTTATTCACGGCGGCAGTTTACAACGTCCGGCGGATGAAACCGGGCTGAGCGAATGGCGAAAATTGCGCCGTGGCCG
CCCGTTGGATGGCATTGTCTGGGCGATGACCGCCGCGCAGAGTGGCACGCCACAGTGGATGGATAACGGCCTGCGAACGC
TGGAAAAAATGGGTGCCGCCTTGCGCTATCAACCGCCGGTGTATCTGTGGCAGGTCTGTGACAGCGACTGGCCTCAGGAT
TCGCGGACGGAGCAGCCGGTCGGGGCGGTGTTCCCGGCGAAGGCTACGCCAGAAAAGGTGGAGCAGCAACTGCGTGCCCT
GTTGCCTCAGTTACGTGAACAGGGAATGCAGCAGCTTTCCATCGAGCCGCACCATGATTTCTTGTTGCGTCTGGCGCAGT
CACTGGATCAGGGCGATGCGGCTCGCTGGCGGCAGCGTCTGACGCCGTGGTTTACCGAGTATGCCGCGCGTATTCCCCTG
CGTGGGCTGATGTTCAGTTTACCCGATACCTCTGCATCGGCGGCGCAGGTACATGAGAAAAGCTGGACGGTACCGGCCAG
TTGGCAGGGGGTACTGGATGATTGCCGGGTGGCGCGTGGACGCCGTGTCGGGCTGCCGTGGGAGCAGACACTGTGCTACA
GCTTGCTGGCACTGATTGTCTTGTGGGGTGTGGGCAGCGTGGTGTCATTTACGGTCAACCGCCACCAGATGGTCTCTGCC
GCACAACAAGCACAGCAACTGGCGCAATCGCACGCAGTATCCGACCAGCAACTGATGGCCTTACAAGCCCTGCGAAATGA
TATCGGGCGCTTACAGGCCCGCGTGGCGCAGGGCGCGCCGTGGTATCAGCGCTTTGGTCTGGACCATAATGCGCCGCTGC
TTGAGGTCCTGATGCCGTGGTATGGTCAGGCCAACAACCGTATTCTCAGGGATGCCACCGCGCAGAGCCTGTATCAGAAA
CTCAGCGAACTGGCGGAGCTGCCCGCCAACAGCCCGCAACGGGCAGCACGGGCAAAAACAGGCTATGACCAGTTAAAGGC
TTATCTGATGATGGCCCGCCCGGAAAAAGCCGATGCTGTATTTTATGCTCAGGTGATGCAGACCACCGAGCCTACACGGG
CCGGTGTCTCCCCCGGTCTGTGGCAGAGTCTGGCGCCCGACTTGTGGCAGTTTTATGCACAAAATCTGCCCGCTCAGCCG
GACTGGAAAATTACCGCGGATACCGGGTTGATCAGCCAGGTGCGGCAGGTGTTGCTGGGGCAGATGGGTCAGCGCAATGC
AGAAAGTACGCTGTATGAAAACATGCTGCTGTCGGTGCGCCGCAACTATGCCGACATGACGCTGATGGACATGACCGGCG
ATACCGATGCTCAGCGTCTGTTTCAGACCTCGGAATCCGTGCCCGGTATGTTCACCCGCAAGGCCTGGGATGAGCAAATC
CAGCAGGCGATAGATAAAACGGTGGCCTCCCGCCGCGAAGAAATTGACTGGGTATTGAGTGATAACCGCCGGGCGATATC
CGAAGATATCTCACCGGAAGCGCTGAAAAAACGCCTGACCGAACGTTATTTCACCGATTTTGCTGGCAGTTGGCTGAGTT
TCCTTAACAGTTTGCACTGGAATGAGGCGCATAACCTGTCGGATGTGATTGACCAACTGACCTTGATGAGTGATGTACGC
CAGTCGCCGCTGATTGCGCTAATGAACACGCTGGCGTGGCAAGGGCAGACCGGGCAGCAGAATCAGGCATTATCGGATTC
GCTGGTGAAGTCTGCCAAGGCGCTGATGAATAAAGACCAGGCTCCGGCGATTGACCAGAGTGCCGGTGGGCCAGTAGGGC
CACTGGACGAGACCTTTGGCCCGTTGCTGGCACTGATGGGCAAAGGCGATGCACAAAACAGGCTGTCGTCGGACAGCTCG
CTGAGCCTGCAAACGTTGCTCACCCGCGTGACCCGGGTGCGGCTTAAACTCCAGCAGGTGGTCAATGCTTCGGACCCGCA
GGAGATGACACAGGTGCTGGCCCAGACCGTTTTTCAGGGCAAAAGTGTTGATCTGACGGACACGCAGGAGTACGGCAGCC
TGATAGCCGCCAGTCTGGGAGAGGAGTGGAGCAGCTTCGGGCAGACGATGTTTGTGCAGCCGCTGACGCAGGCGTGGGAA
ACGGTGCTGCAACCTTCATCGGCCAGCCTTAACGACCAGTGGAAAAACGCGGTGGTGGCCAACTGGAAATCGGCCTTTGA
TGGCCGCTATCCGTTTGCCGCCAGTAAAAGTGATGCTTCATTGCCGATGCTGGCCGAGTTTATCCGCAAGGACAGCGGGC
GCATCGACAGCTTCCTGACCCGTGAACTGGGCGGCGTGCTGCACAAAGAAGGGACGCGCTGGGTGCCGAATAAGGTGAAC
AGCCAGGGGCTGACCTTTAACCCCGCCTTCCTGGCGGCAATTAATCAACTGAGTCAAATCTCCGACATCTTGTTCACTGA
CGGCAGTCAGGGGCTGCGCTTTGAGCTGCTGGCCCGTCCGGTGCCGAATGTGGTGGAAACGAATCTGGCGATTGACGGAC
AGAAATTGCATTATTTCAACCAGATGGAGAGCTGGCAGAGTTTCCGCTGGCCGGGGGAGACCTATAAACCCGGCACGATG
TTGACCTGGACTGGTGTGAGTTCAGGCGCCCGGTTGTACGGTGATTATCAGGGGACATGGGGGCTGATCCGTTGGCTGGA
GCAGGCAAAACAGAAAAAGCTGGATGAAGGGCGCTATCAACTGACCTTCACCACCGCAGATAAACAGCCATTGCAATGGA
TATTACGCACCGAGCTGGGCAAAGGCCCGTTAGGCCTGTTGCAACTGCGTAATTTCACTCTGCCTGCGCAAATTTTTCTG
ATACAGCACGCGCCGCTTGCGATTGCTGACATGTCAGACGATGAAGACATGGCAGAAGACTGA					