SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002560
Component protein type	TssF
T6SS ID (Type)	T6SS00259 (Type i2)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (588 a.a.); Nucleotide sequence (1764 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS12940
Coordinate (Strand)	2842048..2843811 (-)
NCBI ID	WP_012304272.1
RefSeq	NC_010465
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	6.3e-185	1..584

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-587	MDDLTLRYFDAEMRYLREAGEEFARAHPDRAAGLNLDKAGARDPYVERLFEGFAFLMGRMREKLDDDLPELTEGLVSLLWPHYLRTIPSLSIVEFTPQWQEMKERMGVSKGFEVLSRPIGERETRCRYTTTQEMDLLPLTLRRAGLDSEPDGRSVIRLRFDCSALADWSRINLSRLPLYFDADAPLACALHEALTLNTAKLWIRLPGQADRQPLDGHFAPLGFGEQDTLWPKADSAFSGYQLLLEYFTFREKFMFVALQGLDGIELPAELPWFEIDVVLEKRWQHDFSFSEKNLRLHCVPVINLFPLESDPLSLSSLQTEYLLRPMRIQDGYTEVYSVDSVISSRHTGHQVYVPFTSFRHKGGMLRHDAPEYYYHTRVKRGPSGLHDTWLVLGGEAFDNHSVPDNENLSLSLTGTNGQLPRKALQSTVLDTAVKSTGAQVRVRNLSAPSLPCYPPNRDRFHWRVLSHLGSSFLWMMDNAEVLRGTLALYDWTDNEMNRRRLEAIAEVKHSEIERFERGYLLRGVHIEITLDSNGFTGTGDICLFGEMLSRFFALYTDIHLFNRLTLILQPTGERLEWEENHQSRLPG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9965	-	-

Protein sequence: 588 a.a.

						>T6CP002560 NC_010465:c2843811-2842048 [Yersinia pseudotuberculosis YPIII] [TssF]
MDDLTLRYFDAEMRYLREAGEEFARAHPDRAAGLNLDKAGARDPYVERLFEGFAFLMGRMREKLDDDLPELTEGLVSLLW
PHYLRTIPSLSIVEFTPQWQEMKERMGVSKGFEVLSRPIGERETRCRYTTTQEMDLLPLTLRRAGLDSEPDGRSVIRLRF
DCSALADWSRINLSRLPLYFDADAPLACALHEALTLNTAKLWIRLPGQADRQPLDGHFAPLGFGEQDTLWPKADSAFSGY
QLLLEYFTFREKFMFVALQGLDGIELPAELPWFEIDVVLEKRWQHDFSFSEKNLRLHCVPVINLFPLESDPLSLSSLQTE
YLLRPMRIQDGYTEVYSVDSVISSRHTGHQVYVPFTSFRHKGGMLRHDAPEYYYHTRVKRGPSGLHDTWLVLGGEAFDNH
SVPDNENLSLSLTGTNGQLPRKALQSTVLDTAVKSTGAQVRVRNLSAPSLPCYPPNRDRFHWRVLSHLGSSFLWMMDNAE
VLRGTLALYDWTDNEMNRRRLEAIAEVKHSEIERFERGYLLRGVHIEITLDSNGFTGTGDICLFGEMLSRFFALYTDIHL
FNRLTLILQPTGERLEWEENHQSRLPG*					

Nucleotide sequence: 1764 bp

						>T6CP002560 NC_010465:c2843811-2842048 [Yersinia pseudotuberculosis YPIII] [TssF]
ATGGACGACTTAACCCTGCGTTATTTTGACGCTGAAATGCGCTACCTGCGCGAAGCGGGCGAAGAGTTTGCCCGCGCGCA
CCCTGATCGGGCTGCCGGATTAAATCTGGATAAAGCCGGGGCGCGTGACCCCTATGTGGAACGCCTGTTTGAAGGCTTTG
CCTTCCTGATGGGCCGCATGCGAGAAAAACTGGATGATGACCTGCCGGAGCTGACCGAAGGGTTGGTCAGCCTGCTGTGG
CCGCACTACCTGCGTACCATTCCGTCGCTTTCTATTGTGGAGTTCACTCCACAATGGCAGGAGATGAAAGAGCGCATGGG
GGTGAGTAAAGGTTTTGAAGTGCTATCCCGCCCCATTGGTGAACGTGAAACCCGTTGCCGCTATACCACCACGCAAGAGA
TGGACCTGCTCCCTCTGACCCTGCGCCGTGCCGGGCTGGACAGTGAACCCGATGGCCGGTCGGTTATCCGGCTGCGCTTT
GATTGCAGCGCGCTGGCCGACTGGAGCCGTATCAACCTGAGCCGCCTGCCGCTCTATTTTGATGCCGATGCGCCGCTGGC
CTGCGCCCTGCATGAGGCGCTGACCCTGAATACCGCCAAACTCTGGATCCGGTTGCCGGGGCAGGCTGATCGCCAGCCGC
TGGACGGCCATTTCGCGCCGCTGGGGTTCGGGGAGCAGGATACGCTGTGGCCGAAGGCCGACAGTGCGTTCAGTGGTTAT
CAGCTCTTGCTGGAGTACTTTACCTTCCGCGAAAAGTTTATGTTTGTCGCGTTGCAGGGGCTGGATGGGATCGAGCTGCC
GGCGGAACTGCCGTGGTTTGAGATTGATGTGGTGCTGGAAAAACGCTGGCAACATGATTTCTCGTTCTCCGAAAAGAACC
TGCGCCTGCATTGTGTGCCGGTGATTAACCTGTTCCCGCTGGAATCGGACCCACTGTCGCTCTCTTCATTACAGACCGAA
TACCTGCTGCGCCCAATGCGCATTCAGGACGGTTATACCGAAGTGTATTCGGTGGACTCGGTGATCTCCTCCCGTCATAC
CGGGCATCAGGTCTATGTCCCGTTCACCAGCTTTCGCCACAAGGGGGGGATGTTGCGCCATGATGCGCCGGAGTATTACT
ACCATACGCGGGTCAAACGCGGGCCTTCGGGGTTACACGATACCTGGCTGGTGCTGGGGGGCGAGGCGTTTGATAACCAC
AGCGTACCGGATAATGAAAATTTGTCGCTGAGCCTGACCGGCACCAACGGGCAGTTACCGCGTAAAGCGCTGCAAAGCAC
GGTGCTGGATACGGCGGTGAAATCCACCGGGGCTCAGGTGCGGGTACGTAATCTCTCCGCGCCGTCATTGCCATGCTACC
CGCCGAACCGCGACCGTTTTCACTGGCGGGTGCTGAGCCATCTGGGCAGCAGTTTTCTGTGGATGATGGATAACGCCGAA
GTGCTGCGCGGCACGCTGGCGCTGTATGACTGGACGGACAACGAAATGAACCGCCGTCGGCTGGAGGCGATCGCCGAGGT
CAAACACAGCGAGATTGAACGCTTTGAGCGGGGCTATTTGCTGCGCGGGGTGCACATTGAAATCACCCTCGACAGCAACG
GCTTTACCGGTACCGGGGATATTTGTCTGTTTGGGGAAATGCTCAGTCGCTTCTTTGCCCTGTATACCGATATTCACCTG
TTTAATCGTCTGACTCTGATACTGCAACCGACAGGAGAACGTTTGGAATGGGAAGAGAATCATCAGTCCCGCCTTCCCGG
CTAA					