SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002543
Component protein type	TssI
T6SS ID (Type)	T6SS00257 (Type i2)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (783 a.a.); Nucleotide sequence (2349 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS04035
Coordinate (Strand)	916188..918536 (+)
NCBI ID	WP_012303638.1
RefSeq	NC_010465
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	5.5e-59	34..355
DUF2345	PF10106.11	1.7e-50	611..751
T6SS_Vgr	PF13296.8	7.5e-34	492..592
Phage_base_V	PF04717.14	2.2e-09	406..472

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-782	MNTFLPTIRFDHSHHKLVVRDSTAAVDVLGFEGHESLSQPFCYDIQFTSADKAIGPATMLMHDASLTLAAPMAEAFGVTVQQTQRVIQGVVTGFKRLSASKEECRYELSLQPRLALLSRSHQNGIYQDMSVPQIVEKILRERHDMRGQDFVFTLAREYPRREQVMQYGEDDLTFIRRLLAEVGIWFRFTVDPKLNIDVVEFYDDQRFYQQGLTLQAVPPSGMHDSGMESVWDLSSAHQVVEKSVSTGDYNYRTATADLTAGADITRGDTTTYGEAYHYADNYLTAGSEGREPESESGAFYARLRHERYLNNQARFAGVANAAALAPGQELNVTGNDVPAQFGKGVIVTRITSHARRDRSYEVHFEAIPYSEENCFRPILINKPKMAGTLPARVTSTTVNDTYGHIDKDGRYRVNLMFDRDSWEQGYESLWVRQARPYAGDSYGLHLPLLAGTEVAIAFEDGNPDRPYIAYVLHDSVHGDHVTISNYKRNVLRTPSNNKLRLEDERGKEHIKLSTEYGGKSQLNLGHLVDNEKQPRGEGFELRTDSFGVLRAEKGLFITADGQAKAQGQVLEMQPAIGLLQGAQEQMEAISADAQTATASPADLQAQISLLQQNLTELKQAVLLLSAPKGIALSSGEHLQMSASDNLIATAGKNADVSVAKNFFIGVGNTLSIFVRKLGMKLIANQGPITVQAQNDLMELLARKAITITSTEDEIKITAKKRITLNAGGSYITLDENRIESGTAGEYLTKAGYYGRLDKAKLPTEFPALAAKTEDPIKRWLFS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9897	-	-

Protein sequence: 783 a.a.

						>T6CP002543 NC_010465:916188-918536 [Yersinia pseudotuberculosis YPIII] [TssI]
MNTFLPTIRFDHSHHKLVVRDSTAAVDVLGFEGHESLSQPFCYDIQFTSADKAIGPATMLMHDASLTLAAPMAEAFGVTV
QQTQRVIQGVVTGFKRLSASKEECRYELSLQPRLALLSRSHQNGIYQDMSVPQIVEKILRERHDMRGQDFVFTLAREYPR
REQVMQYGEDDLTFIRRLLAEVGIWFRFTVDPKLNIDVVEFYDDQRFYQQGLTLQAVPPSGMHDSGMESVWDLSSAHQVV
EKSVSTGDYNYRTATADLTAGADITRGDTTTYGEAYHYADNYLTAGSEGREPESESGAFYARLRHERYLNNQARFAGVAN
AAALAPGQELNVTGNDVPAQFGKGVIVTRITSHARRDRSYEVHFEAIPYSEENCFRPILINKPKMAGTLPARVTSTTVND
TYGHIDKDGRYRVNLMFDRDSWEQGYESLWVRQARPYAGDSYGLHLPLLAGTEVAIAFEDGNPDRPYIAYVLHDSVHGDH
VTISNYKRNVLRTPSNNKLRLEDERGKEHIKLSTEYGGKSQLNLGHLVDNEKQPRGEGFELRTDSFGVLRAEKGLFITAD
GQAKAQGQVLEMQPAIGLLQGAQEQMEAISADAQTATASPADLQAQISLLQQNLTELKQAVLLLSAPKGIALSSGEHLQM
SASDNLIATAGKNADVSVAKNFFIGVGNTLSIFVRKLGMKLIANQGPITVQAQNDLMELLARKAITITSTEDEIKITAKK
RITLNAGGSYITLDENRIESGTAGEYLTKAGYYGRLDKAKLPTEFPALAAKTEDPIKRWLFS*					

Nucleotide sequence: 2349 bp

						>T6CP002543 NC_010465:916188-918536 [Yersinia pseudotuberculosis YPIII] [TssI]
ATGAATACCTTTCTCCCGACTATTCGGTTTGACCATAGCCACCACAAACTGGTGGTCCGCGACAGCACCGCCGCCGTTGA
TGTGCTGGGCTTTGAAGGCCATGAAAGCCTGAGCCAGCCGTTCTGTTACGACATCCAATTCACCAGCGCCGACAAGGCGA
TTGGCCCGGCGACCATGCTGATGCACGACGCGTCACTGACATTGGCGGCCCCGATGGCCGAAGCTTTTGGCGTGACGGTT
CAGCAGACCCAGCGGGTGATCCAAGGGGTGGTCACCGGATTTAAACGCCTGTCGGCCTCGAAAGAGGAATGCCGCTACGA
ACTGAGCCTGCAACCGCGCCTGGCCTTGTTATCGCGCAGCCATCAGAACGGGATATATCAGGACATGTCGGTGCCGCAGA
TTGTGGAAAAAATTCTGCGTGAGCGCCATGACATGCGCGGTCAGGATTTTGTCTTCACGCTGGCCCGCGAGTACCCACGC
CGCGAGCAGGTGATGCAGTATGGCGAGGATGACCTGACCTTTATCCGCCGCCTGCTGGCCGAGGTGGGGATCTGGTTTCG
TTTTACCGTCGATCCCAAACTCAATATTGATGTGGTGGAGTTTTACGACGACCAGCGCTTCTATCAGCAAGGACTGACGC
TACAGGCGGTGCCGCCTTCGGGGATGCACGACAGCGGGATGGAATCGGTCTGGGACCTGTCCAGCGCCCATCAGGTGGTG
GAAAAATCGGTCAGTACCGGCGATTACAACTACCGCACCGCCACTGCCGACCTGACCGCCGGGGCCGACATCACGCGCGG
CGATACCACCACCTACGGCGAAGCCTATCATTACGCCGATAACTATCTGACCGCAGGCAGCGAAGGGCGCGAGCCGGAAA
GTGAAAGTGGGGCCTTTTATGCCCGTCTGCGCCATGAACGTTACCTCAATAATCAGGCGCGCTTCGCCGGGGTGGCCAAT
GCGGCGGCACTGGCACCGGGTCAGGAACTGAACGTCACCGGCAACGACGTGCCAGCACAATTTGGCAAAGGGGTGATAGT
CACCCGCATCACCAGCCACGCCCGCCGCGACCGCAGCTATGAAGTGCATTTTGAGGCCATCCCTTACTCCGAAGAGAATT
GCTTCCGCCCGATCCTCATCAACAAGCCAAAAATGGCCGGCACCTTGCCCGCACGGGTCACCAGCACCACGGTGAACGAC
ACTTACGGACACATCGACAAAGACGGCCGCTACCGTGTCAATCTGATGTTTGACCGGGACAGTTGGGAGCAAGGTTACGA
AAGCCTGTGGGTCCGTCAGGCCCGCCCGTATGCGGGTGACAGCTACGGCCTGCACCTGCCGCTGCTGGCCGGCACCGAAG
TGGCGATTGCATTTGAGGACGGCAACCCGGACCGGCCGTATATCGCCTATGTGCTGCACGACTCGGTGCACGGCGACCAC
GTCACCATCAGTAATTACAAACGCAACGTACTGCGCACTCCGTCGAACAACAAACTGCGCCTGGAGGATGAACGGGGCAA
AGAGCACATCAAGCTCAGCACCGAATACGGCGGCAAAAGCCAGTTGAATCTGGGGCATTTGGTCGATAACGAGAAACAGC
CTCGGGGTGAGGGTTTTGAGCTGCGCACCGACAGTTTTGGTGTATTACGGGCAGAAAAAGGCCTCTTTATCACTGCCGAC
GGACAGGCCAAAGCACAGGGGCAGGTGCTGGAGATGCAACCGGCTATTGGCCTGTTGCAAGGTGCTCAGGAACAGATGGA
GGCCATCTCCGCGGATGCACAAACCGCCACCGCCAGTCCGGCCGACCTGCAGGCACAAATCAGTTTGTTACAGCAAAATC
TCACTGAACTGAAACAAGCTGTCCTGTTACTGAGTGCGCCAAAGGGGATCGCGTTGAGTAGCGGGGAGCATCTGCAAATG
AGTGCCAGCGATAACCTGATTGCCACCGCCGGTAAAAATGCCGACGTCAGCGTCGCGAAAAACTTCTTTATCGGCGTAGG
CAATACCCTAAGTATCTTCGTCAGAAAGCTGGGGATGAAACTAATAGCCAATCAGGGGCCGATAACAGTCCAGGCGCAGA
ATGATCTAATGGAGTTATTGGCGCGTAAAGCGATCACCATTACCAGCACCGAGGATGAGATAAAAATCACCGCCAAGAAG
AGGATCACGCTGAATGCGGGAGGCAGTTATATCACGCTGGATGAGAATCGGATCGAGTCAGGAACGGCGGGGGAATATTT
GACCAAAGCAGGGTATTACGGGCGGTTAGATAAGGCCAAGCTCCCAACTGAATTTCCAGCCTTAGCCGCTAAAACTGAAG
ACCCCATTAAACGTTGGCTATTCTCTTAA					