SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002539
Component protein type	TssB
T6SS ID (Type)	T6SS00257 (Type i2)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS04000
Coordinate (Strand)	906784..907284 (+)
NCBI ID	WP_002213014.1
RefSeq	NC_010465
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	9.9e-56	8..158

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MSSSSFQNEIPKARINIKLDLHTGGAQKKVELPLKLLVMGDYSNGQEQRPLSEREKLNINKNNFNSVLAEFQPSLKLAVPDTLAGDNTDTAVALTFREMKDFEPESVARQIPQLRALLAMRNLLRDLKSNLLDNATFRRELENILKDDALSDELRAELAALAPKEG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9910	-	-

Protein sequence: 167 a.a.

						>T6CP002539 NC_010465:906784-907284 [Yersinia pseudotuberculosis YPIII] [TssB]
MSSSSFQNEIPKARINIKLDLHTGGAQKKVELPLKLLVMGDYSNGQEQRPLSEREKLNINKNNFNSVLAEFQPSLKLAVP
DTLAGDNTDTAVALTFREMKDFEPESVARQIPQLRALLAMRNLLRDLKSNLLDNATFRRELENILKDDALSDELRAELAA
LAPKEG*					

Nucleotide sequence: 501 bp

						>T6CP002539 NC_010465:906784-907284 [Yersinia pseudotuberculosis YPIII] [TssB]
ATGTCCTCTTCAAGCTTCCAAAATGAGATACCTAAAGCACGCATTAACATCAAATTAGACCTGCATACTGGCGGGGCGCA
AAAGAAAGTCGAACTGCCTTTGAAGTTGTTGGTGATGGGCGATTACAGCAACGGTCAGGAGCAGCGCCCGCTGTCTGAAC
GTGAAAAACTCAATATCAATAAAAACAATTTTAACAGTGTGCTGGCTGAATTCCAACCGAGCCTGAAACTCGCTGTACCG
GACACGCTGGCCGGGGATAACACCGATACAGCCGTCGCTTTGACCTTCCGCGAGATGAAAGATTTCGAACCGGAAAGCGT
CGCCCGTCAAATCCCGCAACTGCGCGCCTTGCTGGCGATGCGCAACCTGCTGCGAGACCTCAAATCCAATCTGCTGGACA
ACGCCACCTTCCGCCGTGAGCTGGAAAATATCCTCAAAGACGATGCCTTGAGCGATGAACTGCGCGCTGAACTGGCGGCC
CTGGCACCAAAAGAGGGTTAA					