SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002530
Component protein type	TssF
T6SS ID (Type)	T6SS00256 (Type i1)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (607 a.a.); Nucleotide sequence (1821 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS01950
Coordinate (Strand)	422127..423947 (+)
NCBI ID	WP_011193201.1
RefSeq	NC_010465
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.5e-150	3..602

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-606	MSFEKYFRDELDYLRQLGRDAAIERPHLAAFLSEQGSDPDVERLLEGFAFLTGNLREKIDDQFPELTHGLLNMLWPNYLRPIPSMTIIEYTPDESVVTEATLVKRGSQVMSIPIAQNGQRSAFINSNAHAGPQCTFTLCRDVWLFPMSIREISANSSNEQGILSVHFAAKAELNLQDLQLDKLRFYLGEDNYTSTQLYFWINHYFERAELVVNGITIPMPDFDFNPVGFERDDALLPYPKNAYMGYRILQEYFCFPEGFLFFDVNGVVDFPPHLNASEFSLNLYFSQALPPDVKVRSTTLRMNCTPAVNLFQHDSEAIDLDGTQTEYPLCVSYHNPDHYDIFSVDRVDSWLSEKGAPKGRGRGHGRVYTSFESFHHQIEHSQEKTALYYRLRVKNSLFRKGFEHYISFVRSDESKLVLAEENVSVSLTCTNRELPLSLRVGDINQLSTDSPSFATFRNITRPSVPLYPVLDGGLHWSLLSNMSLNYMSLLDKDALKQILHTYDFPSLHNRQSARASQKKLDAIQRIETQPIDRLFRGLPVRGLQTTLWLEQGAFSSEGELYLFSTVLARFFSLYASVNAFHLLKVINLDNQECYEWPVQTGQHALM

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9913	-	-

Protein sequence: 607 a.a.

						>T6CP002530 NC_010465:422127-423947 [Yersinia pseudotuberculosis YPIII] [TssF]
MSFEKYFRDELDYLRQLGRDAAIERPHLAAFLSEQGSDPDVERLLEGFAFLTGNLREKIDDQFPELTHGLLNMLWPNYLR
PIPSMTIIEYTPDESVVTEATLVKRGSQVMSIPIAQNGQRSAFINSNAHAGPQCTFTLCRDVWLFPMSIREISANSSNEQ
GILSVHFAAKAELNLQDLQLDKLRFYLGEDNYTSTQLYFWINHYFERAELVVNGITIPMPDFDFNPVGFERDDALLPYPK
NAYMGYRILQEYFCFPEGFLFFDVNGVVDFPPHLNASEFSLNLYFSQALPPDVKVRSTTLRMNCTPAVNLFQHDSEAIDL
DGTQTEYPLCVSYHNPDHYDIFSVDRVDSWLSEKGAPKGRGRGHGRVYTSFESFHHQIEHSQEKTALYYRLRVKNSLFRK
GFEHYISFVRSDESKLVLAEENVSVSLTCTNRELPLSLRVGDINQLSTDSPSFATFRNITRPSVPLYPVLDGGLHWSLLS
NMSLNYMSLLDKDALKQILHTYDFPSLHNRQSARASQKKLDAIQRIETQPIDRLFRGLPVRGLQTTLWLEQGAFSSEGEL
YLFSTVLARFFSLYASVNAFHLLKVINLDNQECYEWPVQTGQHALM*					

Nucleotide sequence: 1821 bp

						>T6CP002530 NC_010465:422127-423947 [Yersinia pseudotuberculosis YPIII] [TssF]
ATGTCGTTCGAAAAGTATTTCAGGGACGAGCTGGACTACCTCCGGCAATTAGGACGCGATGCCGCAATAGAACGGCCTCA
TCTCGCTGCATTTTTATCTGAGCAAGGTTCTGATCCGGATGTTGAACGTTTGCTGGAAGGCTTTGCTTTTCTAACGGGCA
ACCTGCGTGAAAAAATCGATGATCAATTCCCTGAATTGACTCATGGTTTACTGAATATGTTGTGGCCGAACTACTTACGG
CCAATACCGAGCATGACCATCATCGAGTACACACCGGATGAAAGTGTGGTCACCGAGGCGACGCTGGTGAAACGGGGTAG
CCAAGTGATGAGTATCCCCATCGCACAAAATGGTCAGCGCAGCGCGTTTATCAACAGTAATGCTCATGCCGGGCCACAAT
GTACTTTTACGTTGTGCCGTGATGTGTGGTTGTTCCCCATGTCCATCCGGGAGATCTCGGCGAACAGCAGTAACGAACAA
GGCATTTTGAGTGTTCACTTTGCCGCCAAAGCGGAATTGAACTTACAAGACCTGCAACTGGATAAATTGCGCTTTTATCT
GGGCGAAGACAACTACACCAGCACGCAACTCTATTTCTGGATTAACCACTATTTCGAACGCGCTGAACTGGTTGTCAACG
GGATAACCATCCCGATGCCTGATTTTGACTTTAACCCAGTGGGCTTTGAACGCGATGATGCCCTCTTGCCGTACCCGAAA
AACGCCTATATGGGTTACCGGATCCTGCAAGAGTATTTTTGCTTCCCTGAAGGCTTTTTGTTCTTTGATGTCAATGGTGT
GGTCGATTTTCCGCCGCACTTGAATGCCAGTGAATTCTCTCTGAATTTGTATTTCTCTCAGGCATTGCCGCCGGACGTGA
AAGTCCGCTCCACCACACTGCGGATGAATTGCACACCTGCGGTGAACCTGTTCCAACATGACAGTGAAGCGATTGATCTT
GATGGAACCCAGACGGAATATCCGCTCTGTGTCAGCTACCATAATCCGGATCACTACGACATCTTTTCAGTTGATCGGGT
TGACAGTTGGCTCAGTGAAAAAGGTGCTCCTAAGGGGCGCGGCCGGGGTCATGGCAGGGTGTACACCTCGTTTGAGAGCT
TCCACCATCAGATCGAACACTCGCAAGAAAAGACCGCACTTTATTACCGCCTACGTGTGAAAAATTCATTATTCCGTAAG
GGATTCGAACACTACATTTCCTTTGTTCGCAGTGATGAATCGAAGCTGGTGCTTGCTGAAGAGAATGTCTCGGTCAGCCT
GACGTGCACCAACCGTGAGTTGCCGCTGTCGTTGCGCGTTGGTGATATCAATCAACTCAGCACAGACAGCCCTTCATTCG
CGACGTTCCGCAATATTACCCGTCCTTCCGTGCCGTTGTATCCGGTGCTGGATGGGGGCCTGCATTGGTCATTGCTCTCT
AATATGTCGTTGAACTACATGTCCTTACTGGATAAGGACGCGTTGAAGCAAATTCTGCATACCTATGATTTCCCAAGCCT
GCATAACCGGCAATCTGCCAGGGCATCGCAAAAGAAACTGGACGCTATCCAACGTATTGAAACACAACCGATTGACCGGT
TGTTTCGTGGCTTACCTGTCAGAGGGCTACAAACCACGCTATGGCTGGAGCAAGGGGCCTTTAGCTCTGAAGGCGAGCTT
TATCTCTTTAGCACGGTATTAGCTCGCTTCTTCTCACTGTATGCCAGCGTCAATGCGTTTCATCTTTTAAAAGTCATCAA
TCTTGATAATCAGGAATGTTACGAATGGCCGGTACAGACAGGTCAACATGCACTGATGTAA					