SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002491
Component protein type	TssK
T6SS ID (Type)	T6SS00252 (Type i4b)
Strain	Pseudomonas putida GB-1
Replicon	chromosome
Sequence	Protein sequence (448 a.a.); Nucleotide sequence (1344 bp)
Reference	[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PPUTGB1_RS14095
Coordinate (Strand)	3109281..3110624 (+)
NCBI ID	WP_012272404.1
RefSeq	NC_010322
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasE	PF05936.14	8e-136	19..445

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 448 a.a.

						>T6CP002491 NC_010322:3109281-3110624 [Pseudomonas putida GB-1] [TssK]
MSKQSRVMWSEGMFLLPQHFQYQDEFHQHQLAEATLRSTPFHWGVQALQVDEDALANGSLQLKRLKLVFPDGSLYDAPQH
DPLPAARDLKDLLKANDLKVYAALKLPEPFGLNYVEDGHEHKAARRFRKQFDTLPDLNEGELENEITSLRLNVVMLVDGD
SLDGYSYCPLAKLTRNSMGGFNLDPHFVHPTLHLGSHETITSLGKRLLGALQAKSKALSGRRRERADQIAEFGSSDVTLF
WLLNTVNRAHPQLAHLLAHPRLHPERLYLFLADLAGGLLTFTLDTQLSDIPEYDHQDPAASLVKLDEMIRVMLDNVVPNQ
CIVINLTQTKPSHWQGQLHDPRLAEADFYIAVHADMPGASLLELVPRAFKVGSPEDIEVVVNSAMPGVTLNHAARLPNAI
PVRLDNQYFAIEPHGTVYERMINAQAICFYAPSAFTNLKLELMAVLK*					

Nucleotide sequence: 1344 bp

						>T6CP002491 NC_010322:3109281-3110624 [Pseudomonas putida GB-1] [TssK]
ATGAGCAAGCAGAGCCGGGTGATGTGGTCGGAAGGCATGTTCCTGCTGCCCCAGCACTTCCAGTACCAGGACGAGTTCCA
CCAGCACCAGTTGGCTGAGGCGACCCTGCGCAGTACTCCTTTCCATTGGGGTGTACAGGCGCTGCAGGTGGACGAAGACG
CCCTGGCCAACGGTTCGCTGCAGCTCAAGCGCCTGAAGCTGGTGTTCCCTGATGGCAGCCTGTACGACGCTCCGCAGCAC
GATCCGCTGCCGGCCGCGCGTGACCTCAAGGACCTGCTCAAGGCCAACGACCTGAAGGTTTATGCCGCGCTCAAGCTGCC
CGAGCCATTCGGCCTCAATTACGTCGAGGATGGCCACGAGCACAAGGCCGCACGGCGCTTTCGCAAGCAGTTCGACACCC
TGCCCGACCTTAATGAAGGTGAGCTGGAGAACGAGATCACCAGCCTGCGCCTGAACGTGGTCATGCTGGTCGACGGCGAC
AGCCTCGATGGCTACAGCTACTGCCCGCTGGCGAAGCTGACACGCAACAGCATGGGCGGCTTCAACCTCGACCCGCATTT
CGTCCACCCTACCCTGCACCTGGGCAGCCACGAAACCATCACCAGCCTCGGCAAACGCCTGCTCGGTGCCCTGCAGGCCA
AGAGCAAGGCGCTGTCCGGCCGACGCCGCGAGCGCGCCGACCAGATCGCCGAGTTCGGCTCCAGCGACGTCACCTTGTTC
TGGCTGTTGAACACGGTCAACCGTGCCCACCCGCAATTGGCGCATCTGCTGGCCCATCCGCGCCTGCACCCGGAGCGCCT
GTACCTGTTCCTCGCCGACCTGGCCGGCGGGCTCCTGACCTTCACCCTGGACACCCAGCTCAGCGACATCCCCGAGTACG
ACCACCAGGACCCTGCGGCATCGCTGGTGAAACTGGACGAAATGATCCGCGTAATGCTCGACAACGTGGTGCCCAACCAG
TGCATCGTCATCAACCTGACCCAGACCAAACCGTCCCATTGGCAAGGCCAGCTGCACGACCCACGCCTGGCCGAAGCCGA
CTTCTATATTGCCGTGCACGCCGACATGCCCGGTGCCAGCCTGCTGGAACTGGTGCCCCGAGCGTTCAAGGTCGGCTCGC
CGGAAGACATCGAGGTGGTGGTCAACAGCGCCATGCCTGGCGTCACCCTCAATCATGCCGCGCGGTTACCGAACGCGATT
CCGGTGCGCCTGGATAACCAGTATTTCGCCATCGAGCCGCACGGCACTGTGTATGAGCGAATGATCAATGCCCAGGCCAT
CTGCTTCTATGCGCCCAGCGCCTTTACCAACCTCAAGCTTGAACTGATGGCGGTGCTCAAATGA					