SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002254
Component protein type	TssA
T6SS ID (Type)	T6SS00225 (Type i3)
Strain	Yersinia pseudotuberculosis IP 31758
Replicon	chromosome
Sequence	Protein sequence (338 a.a.); Nucleotide sequence (1014 bp)
Reference	[1] Gueguen E, Durand E, Zhang XY, et al. Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator. PLoS One. 2013 Jun 19;8(6):e66615. doi: 10.1371/journal.pone.0066615. Print 2013. PMID: 23840509

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPSIP31758_RS18515
Coordinate (Strand)	3873235..3874248 (-)
NCBI ID	WP_002210482.1
RefSeq	NC_009708
Uniprot ID	Q66EQ2_YERPS, A0A0U1QY97_YERP3, Q0WJG6_YERPE, A0A3G5ITG5_YERPU
KEGG ID	yps:YPTB0640, ype:YPO0499, ypi:YpsIP31758_3437
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.3e-12	21..129

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-337	MILKRMDNNMSDIFPQALFGVEYDPAYGEIESILSQLDESADPLSRPHEPPQINWHHISEQANKLLEQCFDLRVMLWFIRANIHIKGISALYDGFMRINAQTQDTDVVIYPQSEEPPLNSGHAAALGWLSTAQCIAELKTARLTEEHPYILQDLITTEALPNGQERYFVTSSTLFLTVNNYFQQNGLPDLKDQLTKVDMLLEQIESYANQSTESYQLHCEQLRIFLKNNISQLAQLNAPEHDDPETVYQKIDSHESLDTQSFNSNDKSIRSRQEIIMMLDRILEYFQHYEPSHPAPIFIRRTKEMIGMDFYSIVEEILPEAVITLKQFTGKTNLPFR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9824	-	-

Protein sequence: 338 a.a.

						>T6CP002254 NC_009708:c3874248-3873235 [Yersinia pseudotuberculosis IP 31758] [TssA]
MILKRMDNNMSDIFPQALFGVEYDPAYGEIESILSQLDESADPLSRPHEPPQINWHHISEQANKLLEQCFDLRVMLWFIR
ANIHIKGISALYDGFMRINAQTQDTDVVIYPQSEEPPLNSGHAAALGWLSTAQCIAELKTARLTEEHPYILQDLITTEAL
PNGQERYFVTSSTLFLTVNNYFQQNGLPDLKDQLTKVDMLLEQIESYANQSTESYQLHCEQLRIFLKNNISQLAQLNAPE
HDDPETVYQKIDSHESLDTQSFNSNDKSIRSRQEIIMMLDRILEYFQHYEPSHPAPIFIRRTKEMIGMDFYSIVEEILPE
AVITLKQFTGKTNLPFR*					

Nucleotide sequence: 1014 bp

						>T6CP002254 NC_009708:c3874248-3873235 [Yersinia pseudotuberculosis IP 31758] [TssA]
TTGATTTTAAAACGGATGGACAATAATATGAGTGACATATTTCCTCAAGCATTATTCGGCGTTGAATATGATCCGGCCTA
TGGCGAGATTGAGTCCATCCTCTCTCAATTAGATGAGTCTGCGGACCCCCTTTCCCGCCCGCATGAACCCCCTCAAATAA
ACTGGCACCATATCAGTGAGCAAGCCAATAAGCTGCTAGAACAATGTTTTGATTTACGTGTAATGCTTTGGTTTATAAGG
GCAAATATTCATATAAAAGGAATCTCGGCCCTCTATGATGGCTTTATGCGCATTAATGCACAAACCCAAGATACTGATGT
TGTTATCTACCCTCAATCGGAAGAACCGCCATTAAATAGTGGGCATGCCGCCGCATTAGGTTGGTTATCTACAGCACAAT
GTATTGCAGAGCTTAAGACTGCACGCCTTACAGAAGAGCACCCTTACATTTTACAAGATCTTATTACGACTGAAGCGTTA
CCCAATGGTCAAGAGAGATATTTTGTTACATCTTCGACATTATTTTTAACTGTCAATAATTATTTCCAACAGAATGGATT
GCCAGATTTAAAAGACCAATTGACAAAAGTAGATATGTTGTTGGAACAAATTGAGTCCTATGCAAATCAATCCACAGAGA
GTTACCAACTGCATTGTGAACAATTACGTATTTTCTTAAAAAATAATATTTCTCAGCTTGCACAATTGAATGCCCCAGAA
CATGACGATCCCGAAACTGTTTATCAAAAAATAGACAGTCATGAATCTCTTGATACTCAGTCCTTTAATTCAAATGATAA
GAGCATTCGCTCACGGCAAGAGATCATTATGATGCTGGACCGTATTCTAGAGTATTTTCAACATTATGAGCCTAGTCATC
CAGCCCCAATATTCATTCGCCGTACCAAAGAGATGATAGGTATGGATTTTTATTCCATTGTTGAAGAAATTCTGCCTGAA
GCTGTAATTACATTAAAACAGTTTACCGGAAAAACTAACCTACCGTTTCGATGA					