SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002245
Component protein type	TssJ
T6SS ID (Type)	T6SS00225 (Type i3)
Strain	Yersinia pseudotuberculosis IP 31758
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Gueguen E, Durand E, Zhang XY, et al. Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator. PLoS One. 2013 Jun 19;8(6):e66615. doi: 10.1371/journal.pone.0066615. Print 2013. PMID: 23840509

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPSIP31758_RS18450
Coordinate (Strand)	3857218..3857709 (-)
NCBI ID	WP_011191717.1
RefSeq	NC_009708
Uniprot ID	A0A0E8XF05_9GAMM, Q66EN9_YERPS, A0A0U1QY58_YERP3, A0A3G5KES3_YERPU
KEGG ID	yps:YPTB0653, ypq:DJ40_1716, ypu:BZ21_4035, ypi:YpsIP31758_3424, ypr:BZ20_1442, ypo:BZ17_1904
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.2e-18	37..152

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-4	MRSL
Transmembrane helix	5-27	FRVLPIFILTGCVLLSGCSWFSS
Inside	28-163	SSEAALQKRRIDLQVVAAPLINPGPNGQAQPLKVCIIELNKEGWSPPGLYQGTLCSGISVGGEVVSVTEYILAPTEVRQYSRDVPFEQERWWAIAAEFQEMSNGKSLLMLKSDARADFNRVVLVDGKALSLKASTK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9985	pos: 21-22. LSG-CS.	MRSLFRVLPIFILTGCVLLSG

Protein sequence: 164 a.a.

						>T6CP002245 NC_009708:c3857709-3857218 [Yersinia pseudotuberculosis IP 31758] [TssJ]
MRSLFRVLPIFILTGCVLLSGCSWFSSSSEAALQKRRIDLQVVAAPLINPGPNGQAQPLKVCIIELNKEGWSPPGLYQGT
LCSGISVGGEVVSVTEYILAPTEVRQYSRDVPFEQERWWAIAAEFQEMSNGKSLLMLKSDARADFNRVVLVDGKALSLKA
STK*					

Nucleotide sequence: 492 bp

						>T6CP002245 NC_009708:c3857709-3857218 [Yersinia pseudotuberculosis IP 31758] [TssJ]
ATGCGTAGCCTCTTTCGTGTGTTGCCTATTTTTATCCTGACAGGTTGCGTCCTGCTCAGCGGCTGTAGCTGGTTTTCCTC
ATCCTCAGAAGCGGCGCTACAGAAACGGCGGATTGACTTGCAGGTTGTTGCGGCTCCTCTCATCAATCCTGGGCCCAACG
GTCAGGCACAGCCCTTGAAGGTATGCATTATTGAGCTGAATAAAGAGGGGTGGTCACCCCCCGGTTTGTATCAGGGGACG
CTCTGCAGTGGCATCAGTGTAGGAGGTGAGGTTGTCAGCGTGACGGAATACATCCTTGCGCCGACAGAGGTGCGTCAATA
TTCCCGCGATGTTCCCTTTGAACAAGAGCGCTGGTGGGCAATAGCGGCTGAGTTTCAGGAGATGAGTAACGGTAAAAGCC
TATTAATGCTGAAATCTGATGCTCGTGCTGACTTCAACCGTGTGGTGCTCGTTGATGGTAAGGCCCTGTCATTAAAAGCG
TCAACAAAGTAG					