Detailed information of component protein


Summary

Component ID T6CP002228
Component protein type TssL
T6SS ID (Type) T6SS00223 insolico (Type i3)
Strain Yersinia pseudotuberculosis IP 31758
Replicon chromosome
Sequence Protein sequence (459 a.a.); Nucleotide sequence (1377 bp)
Reference
-
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) YPSIP31758_RS08250
Coordinate (Strand) 1608052..1609428 (+)
NCBI ID WP_012104870.1
RefSeq NC_009708
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3e-48 86..289
OmpA PF00691.22 7.1e-17 352..450
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-270 MSDSSDLNLGGSMPPFDQSRNVDESPQALGRFLLDEPITYPDSTQDNAAFTREAPHSGAAQQYDTTLLRGESVQQRVAKVLASSTPLLEAAQPLLRALSEMPAHIGDVSQVHILKQSLKNEITLFSVVCDEADISWKKMAIVRYCLCTALDEAAHTMSWGMMAGWSQSNLLNHFEGDNDGGNKFFLLVGRLSMSPHEYADGLEVLLRILGLGFEGRYSIIEDGDRQLTKIRQGLLTLLQSTRDTIPPALSPHGLIAHQNRRRRGIFVPVR
Transmembrane helix 271-293 VSMLLCGFLIVATFIGCKYFLSV
Inside 294-458 PESDLLKRMAALQRVVVTPPVAAPKLRLSILLKDEIAKQLVSVDETSNNSKVIFKGDSMFMVGSDNVRPEMIDVIKRVAQEVHRVKGAILIVGHTDSMPINKPGFPNNQVLSEKRAANVARYMEQAGIPTDKIRFEGKGETQPVSSNDDATGRSQNRRVEIFVNY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9856 - -


  Protein sequence: 459 a.a.    

>T6CP002228 NC_009708:1608052-1609428 [Yersinia pseudotuberculosis IP 31758] [TssL]
MSDSSDLNLGGSMPPFDQSRNVDESPQALGRFLLDEPITYPDSTQDNAAFTREAPHSGAAQQYDTTLLRGESVQQRVAKV
LASSTPLLEAAQPLLRALSEMPAHIGDVSQVHILKQSLKNEITLFSVVCDEADISWKKMAIVRYCLCTALDEAAHTMSWG
MMAGWSQSNLLNHFEGDNDGGNKFFLLVGRLSMSPHEYADGLEVLLRILGLGFEGRYSIIEDGDRQLTKIRQGLLTLLQS
TRDTIPPALSPHGLIAHQNRRRRGIFVPVRVSMLLCGFLIVATFIGCKYFLSVPESDLLKRMAALQRVVVTPPVAAPKLR
LSILLKDEIAKQLVSVDETSNNSKVIFKGDSMFMVGSDNVRPEMIDVIKRVAQEVHRVKGAILIVGHTDSMPINKPGFPN
NQVLSEKRAANVARYMEQAGIPTDKIRFEGKGETQPVSSNDDATGRSQNRRVEIFVNY*

  Nucleotide sequence: 1377 bp    

>T6CP002228 NC_009708:1608052-1609428 [Yersinia pseudotuberculosis IP 31758] [TssL]
ATGAGTGACAGCAGTGATTTGAACCTTGGGGGCTCAATGCCTCCTTTTGATCAAAGCAGAAATGTGGATGAGAGTCCGCA
GGCATTAGGCCGGTTCTTGCTTGATGAACCAATAACATACCCAGATAGCACTCAGGATAATGCGGCCTTCACTAGAGAGG
CCCCTCATTCTGGTGCGGCACAGCAATATGACACGACCCTCTTACGGGGTGAAAGTGTGCAACAGCGTGTCGCTAAAGTA
CTCGCATCTTCAACGCCATTATTGGAAGCGGCACAGCCACTATTACGTGCCTTGAGCGAGATGCCCGCGCATATTGGGGA
TGTTTCACAGGTTCATATATTAAAACAAAGCCTTAAAAATGAGATCACCCTCTTTAGTGTGGTGTGTGATGAAGCGGATA
TCTCATGGAAAAAAATGGCTATCGTCCGCTATTGCCTGTGTACTGCGTTGGATGAAGCCGCTCACACGATGTCGTGGGGA
ATGATGGCAGGTTGGTCACAAAGCAACCTGTTGAACCATTTTGAAGGCGATAATGACGGCGGGAATAAGTTTTTCTTATT
GGTCGGCCGTCTCTCTATGAGCCCGCATGAATATGCGGATGGTTTAGAGGTTTTGCTGCGCATTTTAGGGCTCGGTTTTG
AGGGGCGCTACAGCATTATCGAAGATGGTGATCGGCAATTAACTAAAATACGCCAAGGGTTGCTGACGCTGCTCCAAAGT
ACCCGAGACACGATCCCACCGGCACTTTCTCCGCATGGGTTGATCGCACATCAAAACAGAAGACGGCGGGGCATTTTTGT
TCCGGTGCGCGTATCCATGCTGTTGTGTGGTTTCCTCATTGTCGCGACCTTTATTGGATGTAAATACTTTCTCTCGGTAC
CGGAAAGTGACTTGCTAAAAAGAATGGCAGCATTGCAACGTGTGGTTGTGACGCCACCCGTTGCCGCACCAAAACTTCGA
CTGTCTATTTTATTAAAAGATGAGATAGCGAAGCAGTTAGTCAGTGTTGATGAAACGAGCAACAACAGCAAAGTCATCTT
TAAAGGCGACTCTATGTTTATGGTCGGCTCGGATAACGTCAGACCTGAAATGATTGACGTAATTAAACGCGTCGCGCAGG
AAGTGCATCGCGTTAAAGGGGCAATCCTCATTGTCGGGCATACAGACTCTATGCCGATTAATAAACCCGGCTTCCCTAAT
AACCAGGTACTTTCCGAAAAACGCGCGGCCAATGTTGCACGCTATATGGAACAGGCCGGTATTCCAACCGACAAAATTCG
GTTTGAGGGCAAGGGGGAAACGCAACCGGTTAGCAGCAATGACGATGCGACAGGACGTTCGCAAAACCGCCGCGTAGAAA
TTTTTGTTAATTATTGA