Detailed information of component protein
Summary
Component ID | T6CP002214 |
Component protein type | TssM |
T6SS ID (Type) | T6SS00222 (Type i2) |
Strain | Yersinia pseudotuberculosis IP 31758 |
Replicon | chromosome |
Sequence | Protein sequence (1141 a.a.); Nucleotide sequence (3423 bp) |
Reference | - |
External database links
Locus tag (Gene) | YPSIP31758_RS05320 |
Coordinate (Strand) | 954541..957963 (+) |
NCBI ID | WP_012104639.1 |
RefSeq | NC_009708 |
Uniprot ID | - |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related | PF06761.14 | 2e-64 | 418..735 |
IcmF_C | PF06744.14 | 7.2e-22 | 999..1098 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-12 | VKRIALPIKKPE |
Transmembrane helix | 13-35 | VWFWIVALLFLLAGAVLCWLVWQ |
Outside | 36-49 | HPERVGLIQGTPQR |
Transmembrane helix | 50-72 | DRWLTGLVVGTGILTLCALLSYV |
Inside | 73-369 | GTRLSGRKHFDEIRQQAQGDDVSLLKENAKVGDIQQSELSHLKTRLRRRYGLFWRYKVRLLMVVGEPDEIAALAPQLAEQGWLEGQRTVLIHGDSVQSPADETGLSEWRKLRRGRPFDGIVWAITAAQSSHPQWMDNGLRTLEKMGATLRYQPPVYLWQVCGSGWPQDTRVEQPVGVVFPAKATPEQVELQLRALLPQLREQGMQQLSVEPHHDFLLRLAQSLEQGDATRWRQRLTPWFTEYAARIPLRGLMFSLPEVSTSAARVHEKNWTASDRWQGILDDCRSARGRRVGLPWEQ |
Transmembrane helix | 370-392 | TLCYSLLALIVLWGVGSVVSFAV |
Outside | 393-1140 | NRHQMVSAAQQAQQLAQSQAVSDQQLMALQALRNDIGRLQSRVAQGAPWYQRFGLDHNAPLLEVLMPWYGQANNRILRDATAQSLHQKLSELAELPANSPQRAVLAKTGYDQLKAYLMMSRPEKADAAFYAQVMQTTEPVRAGVSPGLWQSLAPDLWQFYAQNLPAQPDWKIKPDTGLVSQVRQVLLGQIGQRNAESTLYENMLLSVRRNYADMTLTDMTGDTDAQRLFQTSESVPGMFTRKAWDEQIQQAIDKTVASRREEIDWVLSDNRRAISEDISPEALKKRLTERYFTDFAGSWLSFLNSLHWNEAHNLSDVIDQLTLMSDVRQSPLIALMNTLAWQGQTGQQNQALSDSLVKSAKALMNKDQAPAIDQSAGGPVGPLDETFGPLLALMGKGDAQNRLSSDSSLSLQTLLTRVTRVRLKLQQVVNASNPQEMTQVLAQTVFQGKSVDLTDTQEYGSLIAASLGEEWSSFGQTMFVQPLTQAWETVLQPSSASLNDQWKNAVVANWKSAFDGRYPFAASKSDASLPMLAEFIRKDSGRIDSFLTRELGGVLHKEGTRWVPSKVNSQGLTFNSDFLAAINQLSQVSDILFTDGSQGLRFELLARPVPNVVETHLAIDGQKLHYFNQMESWQSFRWPGDTYKPGTLLTWTGVNSGARLYGDYQGTWGLIRWLEQAKQKKLDEGRYQLTFTTADNLPLQWILRTELGKGPLGLLQLRNFTLPAQIFLIQSAPSAASDRTDDEDMAED |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9950 | - | - |
Protein sequence: 1141 a.a.
>T6CP002214 NC_009708:954541-957963 [Yersinia pseudotuberculosis IP 31758] [TssM]
VKRIALPIKKPEVWFWIVALLFLLAGAVLCWLVWQHPERVGLIQGTPQRDRWLTGLVVGTGILTLCALLSYVGTRLSGRK
HFDEIRQQAQGDDVSLLKENAKVGDIQQSELSHLKTRLRRRYGLFWRYKVRLLMVVGEPDEIAALAPQLAEQGWLEGQRT
VLIHGDSVQSPADETGLSEWRKLRRGRPFDGIVWAITAAQSSHPQWMDNGLRTLEKMGATLRYQPPVYLWQVCGSGWPQD
TRVEQPVGVVFPAKATPEQVELQLRALLPQLREQGMQQLSVEPHHDFLLRLAQSLEQGDATRWRQRLTPWFTEYAARIPL
RGLMFSLPEVSTSAARVHEKNWTASDRWQGILDDCRSARGRRVGLPWEQTLCYSLLALIVLWGVGSVVSFAVNRHQMVSA
AQQAQQLAQSQAVSDQQLMALQALRNDIGRLQSRVAQGAPWYQRFGLDHNAPLLEVLMPWYGQANNRILRDATAQSLHQK
LSELAELPANSPQRAVLAKTGYDQLKAYLMMSRPEKADAAFYAQVMQTTEPVRAGVSPGLWQSLAPDLWQFYAQNLPAQP
DWKIKPDTGLVSQVRQVLLGQIGQRNAESTLYENMLLSVRRNYADMTLTDMTGDTDAQRLFQTSESVPGMFTRKAWDEQI
QQAIDKTVASRREEIDWVLSDNRRAISEDISPEALKKRLTERYFTDFAGSWLSFLNSLHWNEAHNLSDVIDQLTLMSDVR
QSPLIALMNTLAWQGQTGQQNQALSDSLVKSAKALMNKDQAPAIDQSAGGPVGPLDETFGPLLALMGKGDAQNRLSSDSS
LSLQTLLTRVTRVRLKLQQVVNASNPQEMTQVLAQTVFQGKSVDLTDTQEYGSLIAASLGEEWSSFGQTMFVQPLTQAWE
TVLQPSSASLNDQWKNAVVANWKSAFDGRYPFAASKSDASLPMLAEFIRKDSGRIDSFLTRELGGVLHKEGTRWVPSKVN
SQGLTFNSDFLAAINQLSQVSDILFTDGSQGLRFELLARPVPNVVETHLAIDGQKLHYFNQMESWQSFRWPGDTYKPGTL
LTWTGVNSGARLYGDYQGTWGLIRWLEQAKQKKLDEGRYQLTFTTADNLPLQWILRTELGKGPLGLLQLRNFTLPAQIFL
IQSAPSAASDRTDDEDMAED*
VKRIALPIKKPEVWFWIVALLFLLAGAVLCWLVWQHPERVGLIQGTPQRDRWLTGLVVGTGILTLCALLSYVGTRLSGRK
HFDEIRQQAQGDDVSLLKENAKVGDIQQSELSHLKTRLRRRYGLFWRYKVRLLMVVGEPDEIAALAPQLAEQGWLEGQRT
VLIHGDSVQSPADETGLSEWRKLRRGRPFDGIVWAITAAQSSHPQWMDNGLRTLEKMGATLRYQPPVYLWQVCGSGWPQD
TRVEQPVGVVFPAKATPEQVELQLRALLPQLREQGMQQLSVEPHHDFLLRLAQSLEQGDATRWRQRLTPWFTEYAARIPL
RGLMFSLPEVSTSAARVHEKNWTASDRWQGILDDCRSARGRRVGLPWEQTLCYSLLALIVLWGVGSVVSFAVNRHQMVSA
AQQAQQLAQSQAVSDQQLMALQALRNDIGRLQSRVAQGAPWYQRFGLDHNAPLLEVLMPWYGQANNRILRDATAQSLHQK
LSELAELPANSPQRAVLAKTGYDQLKAYLMMSRPEKADAAFYAQVMQTTEPVRAGVSPGLWQSLAPDLWQFYAQNLPAQP
DWKIKPDTGLVSQVRQVLLGQIGQRNAESTLYENMLLSVRRNYADMTLTDMTGDTDAQRLFQTSESVPGMFTRKAWDEQI
QQAIDKTVASRREEIDWVLSDNRRAISEDISPEALKKRLTERYFTDFAGSWLSFLNSLHWNEAHNLSDVIDQLTLMSDVR
QSPLIALMNTLAWQGQTGQQNQALSDSLVKSAKALMNKDQAPAIDQSAGGPVGPLDETFGPLLALMGKGDAQNRLSSDSS
LSLQTLLTRVTRVRLKLQQVVNASNPQEMTQVLAQTVFQGKSVDLTDTQEYGSLIAASLGEEWSSFGQTMFVQPLTQAWE
TVLQPSSASLNDQWKNAVVANWKSAFDGRYPFAASKSDASLPMLAEFIRKDSGRIDSFLTRELGGVLHKEGTRWVPSKVN
SQGLTFNSDFLAAINQLSQVSDILFTDGSQGLRFELLARPVPNVVETHLAIDGQKLHYFNQMESWQSFRWPGDTYKPGTL
LTWTGVNSGARLYGDYQGTWGLIRWLEQAKQKKLDEGRYQLTFTTADNLPLQWILRTELGKGPLGLLQLRNFTLPAQIFL
IQSAPSAASDRTDDEDMAED*
Nucleotide sequence: 3423 bp
>T6CP002214 NC_009708:954541-957963 [Yersinia pseudotuberculosis IP 31758] [TssM]
GTGAAGCGCATTGCCTTACCGATTAAAAAGCCAGAAGTCTGGTTCTGGATTGTTGCTCTGTTATTTTTGCTGGCGGGTGC
CGTGTTGTGCTGGCTGGTCTGGCAACATCCAGAACGGGTAGGATTAATTCAGGGAACACCGCAACGTGATCGCTGGCTGA
CGGGACTGGTGGTGGGAACAGGGATACTGACCTTGTGCGCATTACTGTCGTATGTGGGGACCCGATTATCCGGCAGAAAA
CACTTTGATGAGATACGGCAGCAGGCACAAGGTGATGATGTGTCGTTGCTGAAAGAAAACGCGAAGGTGGGAGATATTCA
GCAGAGCGAACTGTCCCATTTGAAAACCCGTCTGCGCCGCCGTTACGGGCTGTTCTGGCGCTACAAAGTCCGGTTGCTGA
TGGTGGTGGGAGAGCCTGACGAAATCGCTGCATTGGCTCCGCAACTGGCGGAACAGGGCTGGCTGGAAGGGCAGCGTACG
GTGCTGATTCACGGTGATAGTGTCCAAAGTCCAGCCGATGAAACCGGGCTGAGTGAGTGGCGTAAACTGCGCCGTGGCCG
CCCGTTTGATGGCATTGTTTGGGCGATAACCGCCGCGCAAAGTAGTCATCCACAGTGGATGGACAACGGCCTGCGGACGC
TGGAGAAAATGGGCGCAACCTTGCGCTATCAACCGCCGGTGTATCTGTGGCAGGTCTGCGGCAGCGGCTGGCCTCAGGAT
ACGCGGGTGGAGCAGCCGGTCGGGGTGGTGTTCCCGGCGAAGGCCACGCCAGAGCAGGTTGAGCTTCAACTGCGCGCTCT
GTTGCCTCAGTTACGTGAGCAGGGGATGCAGCAGCTTTCTGTCGAACCGCACCATGATTTCTTGTTGCGTCTGGCGCAGT
CGCTGGAGCAGGGCGATGCCACCCGCTGGCGGCAGCGTCTGACGCCGTGGTTTACCGAGTATGCGGCGCGTATTCCCCTG
CGTGGGTTGATGTTCAGTTTGCCGGAGGTATCAACATCGGCGGCGCGTGTGCACGAGAAAAACTGGACTGCATCTGACCG
CTGGCAGGGGATACTGGATGACTGTCGTTCTGCCCGTGGACGCCGTGTCGGGCTGCCGTGGGAGCAGACATTGTGCTACA
GCTTGCTGGCACTGATAGTGCTGTGGGGCGTGGGCAGTGTCGTGTCATTTGCGGTCAACCGTCACCAAATGGTCTCTGCC
GCGCAGCAGGCACAGCAACTGGCGCAGTCGCAAGCGGTCTCTGACCAGCAACTCATGGCCCTACAAGCCCTGCGCAATGA
TATCGGGCGCTTACAATCTCGCGTGGCGCAGGGGGCACCTTGGTATCAGCGCTTTGGCCTGGATCATAATGCGCCGCTGC
TTGAGGTCCTGATGCCGTGGTATGGCCAGGCCAACAACCGCATTCTTAGGGATGCTACCGCGCAAAGCCTGCATCAGAAA
CTCAGTGAACTGGCGGAGCTGCCCGCCAACAGCCCGCAACGTGCGGTACTGGCAAAAACAGGCTATGACCAGTTAAAGGC
CTATCTGATGATGTCACGTCCGGAAAAAGCCGATGCTGCATTTTATGCACAGGTGATGCAGACCACCGAGCCTGTGCGTG
CCGGTGTATCTCCTGGTCTGTGGCAGAGTCTGGCCCCAGACTTATGGCAATTTTATGCGCAAAACCTGCCTGCTCAGCCG
GACTGGAAAATTAAACCGGATACCGGGTTGGTAAGCCAGGTACGGCAGGTCTTACTGGGGCAGATTGGTCAGCGCAATGC
AGAAAGTACGCTGTATGAAAACATGCTGCTATCAGTGCGTCGCAACTATGCCGACATGACGCTGACGGACATGACCGGCG
ACACTGATGCTCAGCGTCTGTTCCAGACCTCGGAATCCGTGCCCGGTATGTTTACCCGCAAGGCCTGGGATGAGCAAATC
CAGCAGGCAATAGATAAAACGGTGGCCTCCCGCCGCGAAGAGATTGACTGGGTATTGAGTGATAACCGCCGGGCGATATC
CGAAGATATCTCACCGGAAGCGCTGAAAAAACGCCTGACCGAACGTTATTTCACCGATTTTGCTGGCAGTTGGCTGAGTT
TCCTCAACAGTTTGCACTGGAATGAGGCGCATAACCTGTCGGATGTGATTGACCAACTGACCTTGATGAGTGATGTACGC
CAGTCGCCGCTGATTGCGCTAATGAACACGCTGGCGTGGCAAGGGCAGACCGGGCAGCAGAATCAGGCATTATCGGATTC
GCTGGTGAAGTCTGCCAAGGCGCTGATGAATAAAGACCAGGCTCCGGCGATTGACCAGAGTGCCGGTGGGCCAGTAGGGC
CACTGGACGAGACCTTTGGCCCGTTGCTGGCACTGATGGGCAAAGGCGATGCACAAAACAGGCTGTCGTCGGACAGCTCG
CTGAGCCTGCAAACGTTGCTCACCCGCGTGACCCGGGTGCGGCTTAAACTCCAGCAAGTGGTTAATGCCTCGAACCCACA
AGAGATGACTCAGGTGCTGGCCCAGACCGTTTTCCAGGGGAAAAGTGTCGACCTGACGGACACGCAGGAGTACGGCAGCC
TGATTGCCGCCAGTCTGGGGGAAGAGTGGAGCAGTTTCGGGCAGACGATGTTTGTTCAGCCGCTGACGCAGGCGTGGGAG
ACCGTGTTGCAACCTTCGTCGGCCAGCCTTAACGACCAGTGGAAAAACGCGGTGGTGGCCAATTGGAAATCAGCCTTTGA
CGGGCGTTACCCGTTTGCCGCCAGTAAAAGCGATGCTTCACTGCCGATGCTGGCCGAATTTATTCGTAAGGATAGCGGGC
GTATCGACAGCTTCCTGACCCGTGAGCTGGGTGGCGTGCTGCATAAAGAAGGGACGCGCTGGGTTCCGAGTAAAGTGAAC
AGCCAGGGGCTAACGTTTAACTCGGACTTTCTGGCGGCCATTAATCAACTGAGTCAAGTCTCCGATATTCTGTTCACTGA
CGGCAGTCAGGGGCTGCGCTTTGAGCTACTGGCACGCCCGGTTCCGAATGTGGTGGAAACCCATTTAGCGATTGATGGAC
AGAAATTGCATTATTTCAACCAGATGGAAAGTTGGCAGAGTTTCCGCTGGCCGGGTGATACCTACAAACCCGGCACGCTA
TTGACCTGGACTGGCGTGAATTCCGGGGCCCGTTTATACGGTGATTATCAGGGGACATGGGGATTGATCCGCTGGCTGGA
ACAGGCAAAACAGAAAAAGCTGGATGAAGGGCGTTATCAACTGACCTTCACCACCGCGGATAACCTACCACTGCAATGGA
TATTACGCACTGAGCTGGGCAAAGGTCCATTAGGTCTGCTGCAACTGCGTAACTTTACCCTGCCTGCGCAAATTTTTCTG
ATACAGAGCGCCCCTTCAGCGGCATCTGATCGGACCGATGATGAGGATATGGCAGAGGATTAA
GTGAAGCGCATTGCCTTACCGATTAAAAAGCCAGAAGTCTGGTTCTGGATTGTTGCTCTGTTATTTTTGCTGGCGGGTGC
CGTGTTGTGCTGGCTGGTCTGGCAACATCCAGAACGGGTAGGATTAATTCAGGGAACACCGCAACGTGATCGCTGGCTGA
CGGGACTGGTGGTGGGAACAGGGATACTGACCTTGTGCGCATTACTGTCGTATGTGGGGACCCGATTATCCGGCAGAAAA
CACTTTGATGAGATACGGCAGCAGGCACAAGGTGATGATGTGTCGTTGCTGAAAGAAAACGCGAAGGTGGGAGATATTCA
GCAGAGCGAACTGTCCCATTTGAAAACCCGTCTGCGCCGCCGTTACGGGCTGTTCTGGCGCTACAAAGTCCGGTTGCTGA
TGGTGGTGGGAGAGCCTGACGAAATCGCTGCATTGGCTCCGCAACTGGCGGAACAGGGCTGGCTGGAAGGGCAGCGTACG
GTGCTGATTCACGGTGATAGTGTCCAAAGTCCAGCCGATGAAACCGGGCTGAGTGAGTGGCGTAAACTGCGCCGTGGCCG
CCCGTTTGATGGCATTGTTTGGGCGATAACCGCCGCGCAAAGTAGTCATCCACAGTGGATGGACAACGGCCTGCGGACGC
TGGAGAAAATGGGCGCAACCTTGCGCTATCAACCGCCGGTGTATCTGTGGCAGGTCTGCGGCAGCGGCTGGCCTCAGGAT
ACGCGGGTGGAGCAGCCGGTCGGGGTGGTGTTCCCGGCGAAGGCCACGCCAGAGCAGGTTGAGCTTCAACTGCGCGCTCT
GTTGCCTCAGTTACGTGAGCAGGGGATGCAGCAGCTTTCTGTCGAACCGCACCATGATTTCTTGTTGCGTCTGGCGCAGT
CGCTGGAGCAGGGCGATGCCACCCGCTGGCGGCAGCGTCTGACGCCGTGGTTTACCGAGTATGCGGCGCGTATTCCCCTG
CGTGGGTTGATGTTCAGTTTGCCGGAGGTATCAACATCGGCGGCGCGTGTGCACGAGAAAAACTGGACTGCATCTGACCG
CTGGCAGGGGATACTGGATGACTGTCGTTCTGCCCGTGGACGCCGTGTCGGGCTGCCGTGGGAGCAGACATTGTGCTACA
GCTTGCTGGCACTGATAGTGCTGTGGGGCGTGGGCAGTGTCGTGTCATTTGCGGTCAACCGTCACCAAATGGTCTCTGCC
GCGCAGCAGGCACAGCAACTGGCGCAGTCGCAAGCGGTCTCTGACCAGCAACTCATGGCCCTACAAGCCCTGCGCAATGA
TATCGGGCGCTTACAATCTCGCGTGGCGCAGGGGGCACCTTGGTATCAGCGCTTTGGCCTGGATCATAATGCGCCGCTGC
TTGAGGTCCTGATGCCGTGGTATGGCCAGGCCAACAACCGCATTCTTAGGGATGCTACCGCGCAAAGCCTGCATCAGAAA
CTCAGTGAACTGGCGGAGCTGCCCGCCAACAGCCCGCAACGTGCGGTACTGGCAAAAACAGGCTATGACCAGTTAAAGGC
CTATCTGATGATGTCACGTCCGGAAAAAGCCGATGCTGCATTTTATGCACAGGTGATGCAGACCACCGAGCCTGTGCGTG
CCGGTGTATCTCCTGGTCTGTGGCAGAGTCTGGCCCCAGACTTATGGCAATTTTATGCGCAAAACCTGCCTGCTCAGCCG
GACTGGAAAATTAAACCGGATACCGGGTTGGTAAGCCAGGTACGGCAGGTCTTACTGGGGCAGATTGGTCAGCGCAATGC
AGAAAGTACGCTGTATGAAAACATGCTGCTATCAGTGCGTCGCAACTATGCCGACATGACGCTGACGGACATGACCGGCG
ACACTGATGCTCAGCGTCTGTTCCAGACCTCGGAATCCGTGCCCGGTATGTTTACCCGCAAGGCCTGGGATGAGCAAATC
CAGCAGGCAATAGATAAAACGGTGGCCTCCCGCCGCGAAGAGATTGACTGGGTATTGAGTGATAACCGCCGGGCGATATC
CGAAGATATCTCACCGGAAGCGCTGAAAAAACGCCTGACCGAACGTTATTTCACCGATTTTGCTGGCAGTTGGCTGAGTT
TCCTCAACAGTTTGCACTGGAATGAGGCGCATAACCTGTCGGATGTGATTGACCAACTGACCTTGATGAGTGATGTACGC
CAGTCGCCGCTGATTGCGCTAATGAACACGCTGGCGTGGCAAGGGCAGACCGGGCAGCAGAATCAGGCATTATCGGATTC
GCTGGTGAAGTCTGCCAAGGCGCTGATGAATAAAGACCAGGCTCCGGCGATTGACCAGAGTGCCGGTGGGCCAGTAGGGC
CACTGGACGAGACCTTTGGCCCGTTGCTGGCACTGATGGGCAAAGGCGATGCACAAAACAGGCTGTCGTCGGACAGCTCG
CTGAGCCTGCAAACGTTGCTCACCCGCGTGACCCGGGTGCGGCTTAAACTCCAGCAAGTGGTTAATGCCTCGAACCCACA
AGAGATGACTCAGGTGCTGGCCCAGACCGTTTTCCAGGGGAAAAGTGTCGACCTGACGGACACGCAGGAGTACGGCAGCC
TGATTGCCGCCAGTCTGGGGGAAGAGTGGAGCAGTTTCGGGCAGACGATGTTTGTTCAGCCGCTGACGCAGGCGTGGGAG
ACCGTGTTGCAACCTTCGTCGGCCAGCCTTAACGACCAGTGGAAAAACGCGGTGGTGGCCAATTGGAAATCAGCCTTTGA
CGGGCGTTACCCGTTTGCCGCCAGTAAAAGCGATGCTTCACTGCCGATGCTGGCCGAATTTATTCGTAAGGATAGCGGGC
GTATCGACAGCTTCCTGACCCGTGAGCTGGGTGGCGTGCTGCATAAAGAAGGGACGCGCTGGGTTCCGAGTAAAGTGAAC
AGCCAGGGGCTAACGTTTAACTCGGACTTTCTGGCGGCCATTAATCAACTGAGTCAAGTCTCCGATATTCTGTTCACTGA
CGGCAGTCAGGGGCTGCGCTTTGAGCTACTGGCACGCCCGGTTCCGAATGTGGTGGAAACCCATTTAGCGATTGATGGAC
AGAAATTGCATTATTTCAACCAGATGGAAAGTTGGCAGAGTTTCCGCTGGCCGGGTGATACCTACAAACCCGGCACGCTA
TTGACCTGGACTGGCGTGAATTCCGGGGCCCGTTTATACGGTGATTATCAGGGGACATGGGGATTGATCCGCTGGCTGGA
ACAGGCAAAACAGAAAAAGCTGGATGAAGGGCGTTATCAACTGACCTTCACCACCGCGGATAACCTACCACTGCAATGGA
TATTACGCACTGAGCTGGGCAAAGGTCCATTAGGTCTGCTGCAACTGCGTAACTTTACCCTGCCTGCGCAAATTTTTCTG
ATACAGAGCGCCCCTTCAGCGGCATCTGATCGGACCGATGATGAGGATATGGCAGAGGATTAA