Detailed information of component protein
Summary
| Component ID | T6CP002197 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00220  (Type i1) |
| Strain | Yersinia pseudotuberculosis IP 31758 |
| Replicon | chromosome |
| Sequence | Protein sequence (182 a.a.); Nucleotide sequence (546 bp) |
| Reference | - |
Bioinformatics investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | YPSIP31758_RS02965 |
| Coordinate (Strand) | 369750..370295 (+) |
| NCBI ID | WP_012104358.1 |
| RefSeq | NC_009708 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 3.3e-30 | 52..172 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MNNARG |
| Transmembrane helix | 7-29 | YLLTWALCVVFLLSGCSGAWEAT |
| Outside | 30-181 | KKVGKVVMDPDIPVGQAADQPSTVRLTLLSDRDTNPNEKGEAAPTEIVVVYLSENSKLLAADYDQLSSDALEKTLGKNYIDHQEYTLLPDQFKALDPIKLEEKNSYLGVIAHYADANRSEWKKIIKIKGIGHNYQVLVHVRSNDVDLRKEEE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9975 | pos: 21-22. LSG-CS. | MNNARGYLLTWALCVVFLLSG |
Protein sequence: 182 a.a.
>T6CP002197 NC_009708:369750-370295 [Yersinia pseudotuberculosis IP 31758] [TssJ]
MNNARGYLLTWALCVVFLLSGCSGAWEATKKVGKVVMDPDIPVGQAADQPSTVRLTLLSDRDTNPNEKGEAAPTEIVVVY
LSENSKLLAADYDQLSSDALEKTLGKNYIDHQEYTLLPDQFKALDPIKLEEKNSYLGVIAHYADANRSEWKKIIKIKGIG
HNYQVLVHVRSNDVDLRKEEE*
MNNARGYLLTWALCVVFLLSGCSGAWEATKKVGKVVMDPDIPVGQAADQPSTVRLTLLSDRDTNPNEKGEAAPTEIVVVY
LSENSKLLAADYDQLSSDALEKTLGKNYIDHQEYTLLPDQFKALDPIKLEEKNSYLGVIAHYADANRSEWKKIIKIKGIG
HNYQVLVHVRSNDVDLRKEEE*
Nucleotide sequence: 546 bp
>T6CP002197 NC_009708:369750-370295 [Yersinia pseudotuberculosis IP 31758] [TssJ]
ATGAACAACGCCAGAGGCTATCTCCTGACGTGGGCATTATGTGTAGTGTTCCTATTGTCTGGGTGCAGCGGTGCATGGGA
AGCCACTAAAAAGGTCGGCAAGGTGGTTATGGACCCCGATATTCCGGTGGGGCAGGCTGCTGATCAACCCTCAACGGTCA
GGCTAACATTGCTGTCCGATCGCGATACCAACCCTAATGAAAAAGGGGAAGCGGCCCCGACTGAGATAGTGGTGGTGTAC
CTAAGTGAAAACTCCAAGCTGCTGGCCGCGGATTATGATCAGCTAAGCAGTGACGCGCTGGAGAAAACGCTGGGTAAGAA
TTACATCGATCATCAGGAATACACCTTACTGCCCGATCAATTCAAGGCGCTGGACCCTATCAAGCTGGAAGAGAAAAACA
GCTATCTGGGGGTGATTGCTCACTATGCCGATGCCAACCGCAGTGAATGGAAGAAGATCATCAAAATAAAAGGGATCGGC
CATAACTACCAGGTTTTGGTCCATGTCAGATCAAACGATGTCGATCTAAGGAAAGAGGAAGAATAA
ATGAACAACGCCAGAGGCTATCTCCTGACGTGGGCATTATGTGTAGTGTTCCTATTGTCTGGGTGCAGCGGTGCATGGGA
AGCCACTAAAAAGGTCGGCAAGGTGGTTATGGACCCCGATATTCCGGTGGGGCAGGCTGCTGATCAACCCTCAACGGTCA
GGCTAACATTGCTGTCCGATCGCGATACCAACCCTAATGAAAAAGGGGAAGCGGCCCCGACTGAGATAGTGGTGGTGTAC
CTAAGTGAAAACTCCAAGCTGCTGGCCGCGGATTATGATCAGCTAAGCAGTGACGCGCTGGAGAAAACGCTGGGTAAGAA
TTACATCGATCATCAGGAATACACCTTACTGCCCGATCAATTCAAGGCGCTGGACCCTATCAAGCTGGAAGAGAAAAACA
GCTATCTGGGGGTGATTGCTCACTATGCCGATGCCAACCGCAGTGAATGGAAGAAGATCATCAAAATAAAAGGGATCGGC
CATAACTACCAGGTTTTGGTCCATGTCAGATCAAACGATGTCGATCTAAGGAAAGAGGAAGAATAA