SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001615
Component protein type	TssH
T6SS ID (Type)	T6SS00164 (Type i3)
Strain	Acidovorax citrulli AAC00-1
Replicon	chromosome
Sequence	Protein sequence (926 a.a.); Nucleotide sequence (2778 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Tian Y, Zhao Y, Wu X, et al. The type VI protein secretion system contributes to biofilm formation and seed-to-seedling transmission of Acidovorax citrulli on melon. Mol Plant Pathol. 2015 Jan;16(1):38-47. doi: 10.1111/mpp.12159. Epub 2014 Jul 16. PMID: 24863458

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AAVE_RS07365
Coordinate (Strand)	1640211..1642988 (+)
NCBI ID	WP_011794623.1
RefSeq	NC_008752
Uniprot ID	A1TM85_ACIAC
KEGG ID	aav:Aave_1482
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	3e-39	655..821
AAA_lid_9	PF17871.3	1.1e-27	373..462
ClpB_D2-small	PF10431.11	5.5e-14	830..903
AAA	PF00004.31	6e-09	233..335

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-925	MSEISRQALFGKLNPLLFKSLESATVFCKLRGNPYVELVHWLHQLLQETDSDLIRLLRHFAVDSDRLATDVLQSLDRLPRGATSISDFSAQIESSIERGWVYATLMYGESQIRSAYWLSGMLRTRVLANELRAISGEFNKLGEPALSEVFGRVLPDSPESRLKAADGSGLGGVPGEASGATAEAAGPGEALKRYTVDLTERARLQELDPVTGRDEEVRQMVDILMRRRQNNPILVGEAGVGKTAVVEGLALRIAAGDVPPPLKDVQLRVVDIGLLQAGASMKGEFENRLRSLLDEVQASTVPIVLFIDEVHTLVGAGGQAGTGDAANLLKPALARGVLRTIGATTWAEYKKYIEKDPALTRRFQLVQIHEPDEAKAVHMLRGVAAKLEGHHKVRIGEDAVAAAVTLSHRYIPSRQLPDKAVSLLDTACSRVALGLHATPARLESVVRQLEALEVEKDIVQRELGLGVGDAGRFADIGTAIERLLAEKAELQERFEREKVLVQQIVDAEAALLKPAGKPGAAGAAQAPAPATAPAHASEASGEAGSGGAAAAGTAQGAAVSPGVATPEALAALRQDLARLQGEDPLLQPVVDASVVAAVVAEWTGIPVGRMVRDEVQSVLTLADTLEKRVIGQRHGLEAITRRIQTARAQLDNPGKPIGVFLLAGPSGVGKTETALSLAEALYGGEQNLITINMSEYQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEIEKAHPDVHELFFQVFDKGWMEDGEGRYIDFKNTVIILTSNVGSELIAGMCRDPELAPQPEALAQAVRKPLLEKFPAALLGRLVVVPYYPISDAMLEQIIRLQLQRIQTRIAERHGAVLDIDDAAVQLIRQRCTEVESGARMVDGIITQNLLPRLAMRFLQSSVDSKSIRRIRLGVADSDFAIEYEEA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9923	-	-

Protein sequence: 926 a.a.

						>T6CP001615 NC_008752:1640211-1642988 [Acidovorax citrulli AAC00-1] [TssH]
MSEISRQALFGKLNPLLFKSLESATVFCKLRGNPYVELVHWLHQLLQETDSDLIRLLRHFAVDSDRLATDVLQSLDRLPR
GATSISDFSAQIESSIERGWVYATLMYGESQIRSAYWLSGMLRTRVLANELRAISGEFNKLGEPALSEVFGRVLPDSPES
RLKAADGSGLGGVPGEASGATAEAAGPGEALKRYTVDLTERARLQELDPVTGRDEEVRQMVDILMRRRQNNPILVGEAGV
GKTAVVEGLALRIAAGDVPPPLKDVQLRVVDIGLLQAGASMKGEFENRLRSLLDEVQASTVPIVLFIDEVHTLVGAGGQA
GTGDAANLLKPALARGVLRTIGATTWAEYKKYIEKDPALTRRFQLVQIHEPDEAKAVHMLRGVAAKLEGHHKVRIGEDAV
AAAVTLSHRYIPSRQLPDKAVSLLDTACSRVALGLHATPARLESVVRQLEALEVEKDIVQRELGLGVGDAGRFADIGTAI
ERLLAEKAELQERFEREKVLVQQIVDAEAALLKPAGKPGAAGAAQAPAPATAPAHASEASGEAGSGGAAAAGTAQGAAVS
PGVATPEALAALRQDLARLQGEDPLLQPVVDASVVAAVVAEWTGIPVGRMVRDEVQSVLTLADTLEKRVIGQRHGLEAIT
RRIQTARAQLDNPGKPIGVFLLAGPSGVGKTETALSLAEALYGGEQNLITINMSEYQEAHTVSTLKGAPPGYVGYGEGGV
LTEAVRRRPYSVVLLDEIEKAHPDVHELFFQVFDKGWMEDGEGRYIDFKNTVIILTSNVGSELIAGMCRDPELAPQPEAL
AQAVRKPLLEKFPAALLGRLVVVPYYPISDAMLEQIIRLQLQRIQTRIAERHGAVLDIDDAAVQLIRQRCTEVESGARMV
DGIITQNLLPRLAMRFLQSSVDSKSIRRIRLGVADSDFAIEYEEA*					

Nucleotide sequence: 2778 bp

						>T6CP001615 NC_008752:1640211-1642988 [Acidovorax citrulli AAC00-1] [TssH]
ATGTCTGAAATCAGCCGGCAAGCCCTTTTCGGCAAGCTCAATCCCCTGCTCTTCAAGTCGCTGGAAAGCGCGACCGTGTT
CTGCAAGCTGCGCGGCAATCCCTACGTGGAGCTGGTGCACTGGCTGCACCAGCTGCTGCAGGAGACGGATTCCGACCTGA
TCCGGCTGCTGCGCCACTTCGCGGTGGATTCCGACCGGCTCGCCACCGACGTGCTGCAGAGCCTGGACCGGCTGCCGCGC
GGCGCCACGTCGATCAGCGATTTCTCGGCGCAGATCGAATCCTCGATCGAACGCGGCTGGGTGTACGCCACGCTGATGTA
CGGCGAATCGCAGATCCGCAGCGCCTACTGGCTCTCGGGCATGCTGCGCACGCGCGTGCTGGCCAACGAGCTGCGCGCGA
TTTCGGGGGAATTCAACAAGCTGGGCGAGCCTGCCCTGAGCGAGGTGTTCGGCCGCGTGCTGCCGGATTCGCCGGAATCG
CGCCTGAAGGCCGCGGACGGCAGCGGCCTGGGCGGCGTGCCCGGCGAGGCGAGCGGCGCCACTGCCGAGGCGGCCGGGCC
CGGCGAGGCCCTGAAGCGTTACACGGTGGACCTGACGGAGCGCGCGCGCCTGCAGGAACTCGACCCGGTCACCGGCCGCG
ACGAGGAAGTGCGCCAGATGGTGGACATCCTCATGCGCCGGCGTCAGAACAACCCCATCCTGGTCGGCGAGGCCGGCGTG
GGCAAGACGGCGGTGGTGGAAGGCCTCGCGCTGCGCATCGCGGCGGGCGACGTGCCGCCACCGCTCAAGGACGTGCAACT
GCGCGTGGTGGACATCGGCCTGCTGCAGGCCGGCGCGAGCATGAAGGGCGAGTTCGAGAACCGCCTGCGCTCGCTGCTCG
ACGAGGTGCAGGCCAGCACGGTGCCGATCGTGCTGTTCATCGACGAGGTGCACACGCTCGTCGGCGCGGGCGGGCAGGCA
GGCACGGGCGATGCCGCCAACCTGCTCAAGCCGGCGCTGGCGCGCGGCGTGCTGCGCACCATCGGCGCCACCACCTGGGC
CGAGTACAAGAAATACATCGAGAAGGACCCGGCGCTCACCCGGCGCTTCCAGCTCGTGCAGATCCACGAGCCGGACGAGG
CCAAGGCGGTGCACATGCTGCGCGGCGTGGCCGCGAAGCTGGAAGGCCATCACAAGGTGCGTATCGGCGAGGACGCCGTG
GCTGCGGCGGTGACGCTGTCGCACCGCTACATCCCGTCGCGCCAGCTGCCCGACAAGGCCGTGAGCCTGCTGGACACCGC
GTGCTCGCGCGTGGCGCTGGGCCTGCACGCGACGCCGGCGCGGCTGGAGTCGGTGGTGCGTCAGCTGGAGGCGCTCGAGG
TCGAGAAGGACATCGTGCAGCGCGAGCTGGGCCTGGGCGTGGGCGACGCCGGCCGCTTCGCCGACATCGGCACCGCGATC
GAGCGCCTGCTGGCCGAAAAGGCCGAATTGCAGGAGCGGTTCGAACGCGAGAAGGTGCTGGTGCAGCAGATCGTGGACGC
GGAGGCGGCGTTGCTGAAGCCGGCCGGCAAGCCCGGGGCAGCCGGGGCGGCGCAGGCGCCTGCCCCGGCAACCGCGCCGG
CCCATGCATCGGAGGCCTCCGGAGAAGCCGGCAGCGGCGGGGCGGCTGCCGCCGGTACGGCACAGGGCGCGGCGGTGTCC
CCGGGCGTCGCCACTCCCGAGGCGCTGGCTGCGCTGCGCCAGGACCTGGCGCGCCTGCAGGGCGAAGATCCGCTGCTGCA
GCCCGTCGTGGATGCGAGCGTGGTGGCTGCGGTGGTGGCCGAATGGACGGGCATCCCCGTGGGCCGCATGGTGCGCGACG
AGGTGCAGTCCGTGCTGACGCTCGCCGATACGCTGGAGAAGCGGGTGATCGGCCAGCGCCACGGCCTGGAGGCGATCACG
CGCCGCATCCAGACCGCGCGTGCCCAGCTGGACAACCCCGGCAAGCCCATCGGCGTGTTCCTGCTGGCCGGGCCCTCGGG
CGTCGGCAAGACGGAGACCGCGCTGTCGCTGGCCGAGGCGCTGTACGGTGGCGAGCAGAACCTCATCACCATCAACATGA
GCGAATACCAGGAGGCCCACACGGTCTCCACGCTCAAGGGCGCGCCGCCCGGCTACGTGGGCTACGGCGAAGGCGGCGTG
CTGACCGAGGCCGTGCGCCGCCGCCCCTACAGCGTGGTGCTGCTCGACGAGATCGAGAAGGCCCACCCGGACGTGCACGA
GCTGTTCTTCCAGGTGTTCGACAAGGGCTGGATGGAAGACGGCGAGGGGCGCTACATCGATTTCAAGAACACGGTCATCA
TCCTGACTTCCAACGTGGGCTCGGAGCTGATCGCCGGCATGTGCCGGGATCCGGAGCTGGCGCCCCAGCCCGAGGCACTG
GCCCAGGCGGTGCGCAAGCCGCTGCTGGAGAAATTCCCGGCCGCGCTGCTGGGCCGGCTGGTGGTGGTGCCGTACTACCC
GATCTCGGACGCGATGCTCGAGCAGATCATCCGCCTGCAGCTGCAGCGCATCCAGACGCGCATCGCCGAGCGCCACGGCG
CCGTGCTGGACATCGACGATGCGGCCGTGCAGCTCATTCGCCAGCGCTGCACCGAGGTGGAATCCGGCGCCCGGATGGTG
GACGGAATCATCACCCAGAACCTGCTGCCGCGGCTGGCGATGCGCTTCCTGCAGAGCTCTGTCGATTCGAAGTCGATCCG
CCGCATCCGCCTCGGCGTGGCGGACAGCGATTTCGCCATCGAGTACGAAGAGGCCTGA					