Detailed information of component protein


Summary

Component ID T6CP001608
Component protein type TssM
T6SS ID (Type) T6SS00164 experimental (Type i3)
Strain Acidovorax citrulli AAC00-1
Replicon chromosome
Sequence Protein sequence (1210 a.a.); Nucleotide sequence (3630 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AAVE_RS07320
Coordinate (Strand) 1627945..1631574 (+)
NCBI ID WP_011794614.1
RefSeq NC_008752
Uniprot ID A1TM76_ACIAC
KEGG ID aav:Aave_1473
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.6e-93 208..467
IcmF-related PF06761.14 1.2e-85 519..832
IcmF_C PF06744.14 4.5e-26 1085..1190
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 MLRKIFSF
Transmembrane helix 9-31 FFNRFTLVLCGLVLLSLLVWFVG
Outside 32-45 PLVYIKPYQPLESE
Transmembrane helix 46-65 AVRGWIIAALFGIWFLRLFI
Inside 66-458 RWWRAKEMNARLLGQLARIGASDASAPEAGPGKEEVAELEKRFKEAVDVLGKTRFGQTEQGWFGRLSRRYLYQLPWYLIIGSPGSGKTTALVNSGLDFPLAAQFGKASIRGVGGTRNCDWWFTDQAVLLDTAGRYTTQESDEAQDSAAWSGFLGLLRRFRGRQPINGVLVTLSVQELLSGGDAERERLARLIGLRLAELCEGLSIKFPVYVLVTKTDLLAGFNEFFGNMTREERAQVWGFTNPYVEGQAQEDPGLQFRKEFDGLADQINRLLPQRLLAEPDLARRGQIYGLPQQFVGLRDVVQQTLSTIFASSRFKEKPLFRGVYFTSGTQEGMPFDRVLSALSRRFSVAPPAHPAGEGRAGKSYFIETLLKGVIFNEAGLTGRNAKKERQLR
Transmembrane helix 459-481 LLQAAGFFALAAGLAGATIAWTI
Outside 482-1209 SHGNNQKYLDEVAAKVPTLKQAVEESRDADPENMVALLPLLNHAEALAVSQRYTGDSPPLSWRYGLLQVPKVQTAADATYMRLLEDAWLPRLARHLRNSLQQASTANPEASYEALKVYLMLYDPERFNPRVVKAWMLNEWEATLPPALVQSGMLDQLAHHLDRLMEDRALVSPIPIDQPLVDEVRQRLAQLSPAQRAYSRLKQLLTTGTALPPDFTLVRAAGPEAPQVFTRRSGKPLTQGISGLFTYDGYYGVFSHELPKVTSLLAQEETWVLGKAQGQRSVANEVMTGQLALEVKRLYLMEYAKVWEDFLADVRPVQVASLDQAGEQARLYSSANSSLEQFIRAVAKETTLGQKPGAGGSSTSSWLGEKINRIKEEQEQLSRLTGKRVNIGGLAASTNLEADLVDFRFREYRRLATSNGSGPAPIAASLQVLNEAAAVISSARQQISAGGTVPPSLSPALERVRMEAKRVPPPLNTMYEDLASSTSAMVGRDVRATVGSNLNATIGTFCRRAIGGRYPFTKGSASDVTSDDFASLFSPGGMMDEFFRNTLQPMVDISTTPWRFRQGVDGTPVGGSAALASFQRAATIRDVYFRAGGKVPSIRMDIKPLEMDASISQMVLDIDGEVLRYQHGPQIPKSMAWPGTRGTGQVRLQITGGGAENTGLVTEGPWAVHRFFDKAQILPGSTPERFVAAFDIGGRKLRFEVTTGSVLNPFRLKAMEEFACPGNL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9729 - -


  Protein sequence: 1210 a.a.    

>T6CP001608 NC_008752:1627945-1631574 [Acidovorax citrulli AAC00-1] [TssM]
MLRKIFSFFFNRFTLVLCGLVLLSLLVWFVGPLVYIKPYQPLESEAVRGWIIAALFGIWFLRLFIRWWRAKEMNARLLGQ
LARIGASDASAPEAGPGKEEVAELEKRFKEAVDVLGKTRFGQTEQGWFGRLSRRYLYQLPWYLIIGSPGSGKTTALVNSG
LDFPLAAQFGKASIRGVGGTRNCDWWFTDQAVLLDTAGRYTTQESDEAQDSAAWSGFLGLLRRFRGRQPINGVLVTLSVQ
ELLSGGDAERERLARLIGLRLAELCEGLSIKFPVYVLVTKTDLLAGFNEFFGNMTREERAQVWGFTNPYVEGQAQEDPGL
QFRKEFDGLADQINRLLPQRLLAEPDLARRGQIYGLPQQFVGLRDVVQQTLSTIFASSRFKEKPLFRGVYFTSGTQEGMP
FDRVLSALSRRFSVAPPAHPAGEGRAGKSYFIETLLKGVIFNEAGLTGRNAKKERQLRLLQAAGFFALAAGLAGATIAWT
ISHGNNQKYLDEVAAKVPTLKQAVEESRDADPENMVALLPLLNHAEALAVSQRYTGDSPPLSWRYGLLQVPKVQTAADAT
YMRLLEDAWLPRLARHLRNSLQQASTANPEASYEALKVYLMLYDPERFNPRVVKAWMLNEWEATLPPALVQSGMLDQLAH
HLDRLMEDRALVSPIPIDQPLVDEVRQRLAQLSPAQRAYSRLKQLLTTGTALPPDFTLVRAAGPEAPQVFTRRSGKPLTQ
GISGLFTYDGYYGVFSHELPKVTSLLAQEETWVLGKAQGQRSVANEVMTGQLALEVKRLYLMEYAKVWEDFLADVRPVQV
ASLDQAGEQARLYSSANSSLEQFIRAVAKETTLGQKPGAGGSSTSSWLGEKINRIKEEQEQLSRLTGKRVNIGGLAASTN
LEADLVDFRFREYRRLATSNGSGPAPIAASLQVLNEAAAVISSARQQISAGGTVPPSLSPALERVRMEAKRVPPPLNTMY
EDLASSTSAMVGRDVRATVGSNLNATIGTFCRRAIGGRYPFTKGSASDVTSDDFASLFSPGGMMDEFFRNTLQPMVDIST
TPWRFRQGVDGTPVGGSAALASFQRAATIRDVYFRAGGKVPSIRMDIKPLEMDASISQMVLDIDGEVLRYQHGPQIPKSM
AWPGTRGTGQVRLQITGGGAENTGLVTEGPWAVHRFFDKAQILPGSTPERFVAAFDIGGRKLRFEVTTGSVLNPFRLKAM
EEFACPGNL*

  Nucleotide sequence: 3630 bp    

>T6CP001608 NC_008752:1627945-1631574 [Acidovorax citrulli AAC00-1] [TssM]
ATGCTGCGCAAGATCTTTTCATTCTTCTTCAACCGGTTCACGCTGGTGCTGTGCGGCCTGGTGCTGCTCAGCCTGCTGGT
CTGGTTCGTCGGTCCGCTGGTCTATATCAAGCCCTACCAGCCGCTCGAGAGCGAGGCGGTGCGCGGCTGGATCATCGCCG
CGCTGTTCGGCATCTGGTTCCTGCGGCTGTTCATCCGGTGGTGGCGGGCCAAGGAGATGAATGCACGCCTGCTGGGCCAG
CTGGCCCGCATCGGCGCATCGGATGCCTCCGCCCCCGAGGCCGGTCCCGGCAAGGAGGAGGTGGCCGAACTCGAGAAGCG
CTTCAAGGAGGCGGTCGATGTCCTGGGCAAGACCCGCTTCGGGCAGACGGAGCAGGGCTGGTTCGGCCGGCTGTCCCGCC
GCTACCTCTACCAGTTGCCCTGGTACCTCATCATCGGTTCTCCGGGATCCGGCAAGACCACGGCACTGGTCAATTCGGGC
CTCGATTTTCCTCTGGCCGCCCAGTTCGGCAAGGCGTCGATCCGCGGCGTCGGCGGCACGCGCAACTGCGACTGGTGGTT
CACCGACCAGGCCGTGCTGCTGGATACCGCCGGCCGCTACACCACGCAGGAGAGCGACGAGGCGCAGGACAGTGCCGCCT
GGTCGGGGTTCCTCGGCCTGCTGCGGCGCTTTCGCGGGCGGCAGCCCATCAACGGGGTGCTGGTCACCCTCAGCGTGCAG
GAGCTGCTGAGCGGCGGCGATGCGGAGCGCGAGCGCCTGGCCCGGCTGATCGGCCTGCGCCTGGCCGAGCTGTGCGAGGG
GCTGTCCATCAAGTTCCCGGTGTACGTGCTGGTCACCAAGACCGACCTGCTGGCCGGCTTCAACGAGTTCTTCGGCAACA
TGACCCGCGAGGAGCGGGCACAGGTCTGGGGCTTCACCAATCCCTATGTGGAAGGGCAGGCGCAGGAAGACCCGGGGCTG
CAGTTCCGCAAGGAATTCGACGGCCTGGCCGACCAGATCAACCGGTTGCTGCCGCAGCGCCTGCTGGCGGAGCCCGACCT
CGCGCGCCGTGGCCAGATCTATGGCCTGCCGCAGCAGTTCGTCGGCCTGCGGGACGTGGTCCAGCAGACGCTGTCCACGA
TCTTCGCGTCCTCGCGTTTCAAGGAAAAGCCGCTGTTCCGCGGCGTCTATTTCACCAGTGGCACGCAGGAGGGCATGCCG
TTCGACCGGGTGCTGTCCGCCCTGTCGCGGCGCTTCTCGGTGGCGCCTCCGGCCCATCCCGCCGGGGAAGGGCGCGCGGG
CAAGAGCTATTTCATCGAGACCCTGCTCAAGGGGGTCATCTTCAACGAGGCCGGGCTGACGGGACGCAATGCCAAGAAGG
AGCGCCAGTTGCGGCTGCTGCAGGCCGCCGGCTTTTTCGCCCTGGCCGCGGGGCTGGCGGGCGCCACGATCGCCTGGACG
ATCAGCCACGGCAACAACCAGAAATACCTGGACGAGGTGGCCGCGAAGGTGCCCACGCTCAAGCAGGCGGTGGAGGAATC
CCGCGACGCGGATCCCGAGAACATGGTGGCGCTGCTGCCGCTGCTGAACCACGCCGAGGCCCTCGCCGTGAGCCAGCGCT
ACACCGGCGATTCGCCGCCGCTCAGCTGGCGCTACGGATTGCTGCAGGTGCCCAAGGTGCAGACCGCTGCCGACGCGACC
TACATGCGGCTGCTGGAGGATGCGTGGCTGCCCCGCCTCGCGCGGCACCTGCGCAATTCGCTGCAGCAGGCCTCGACGGC
CAACCCCGAGGCCAGCTACGAGGCGCTCAAGGTCTATCTGATGCTCTACGATCCGGAGCGCTTCAACCCCCGCGTGGTGA
AGGCCTGGATGCTCAACGAGTGGGAAGCCACGCTGCCCCCGGCCCTGGTGCAGTCGGGCATGCTGGACCAGCTGGCGCAC
CACCTCGACCGGCTCATGGAAGACCGTGCCCTGGTTTCGCCGATTCCGATAGACCAGCCGCTGGTGGACGAGGTGCGCCA
ACGGCTGGCGCAGCTGTCGCCTGCGCAGCGGGCCTACAGCCGGCTGAAGCAGTTGCTGACCACGGGCACGGCGCTGCCGC
CGGACTTCACGCTGGTGCGGGCCGCCGGCCCCGAGGCGCCGCAGGTGTTCACGCGGCGCAGCGGCAAGCCGCTCACTCAG
GGGATTTCTGGACTGTTCACCTACGACGGCTACTACGGCGTGTTCTCGCATGAGCTGCCCAAGGTGACCTCGCTGCTGGC
GCAGGAGGAAACCTGGGTGCTGGGCAAGGCGCAGGGGCAGCGCAGCGTGGCCAACGAGGTCATGACCGGGCAGCTCGCGC
TCGAGGTCAAGCGCCTCTACCTCATGGAGTACGCCAAGGTCTGGGAGGACTTCCTCGCCGATGTGCGGCCCGTGCAGGTG
GCGTCGCTCGACCAGGCCGGCGAGCAGGCGCGGCTGTATTCGTCCGCCAATTCCAGCCTGGAGCAGTTCATCCGCGCGGT
GGCCAAGGAAACCACGCTGGGTCAGAAGCCGGGGGCCGGCGGCAGCAGCACCAGCAGCTGGCTGGGCGAGAAGATCAACC
GCATCAAGGAGGAGCAGGAGCAGCTCAGCCGCCTCACCGGCAAGCGGGTGAACATCGGCGGGCTCGCGGCCAGCACCAAC
CTCGAGGCCGATCTGGTGGACTTCCGCTTCCGCGAATACCGCCGTCTCGCCACCTCCAACGGATCGGGCCCGGCGCCCAT
CGCCGCCAGCCTGCAGGTGCTCAACGAAGCCGCCGCGGTCATCTCGTCCGCGCGCCAGCAGATCAGTGCCGGCGGCACCG
TGCCGCCGTCGCTCTCGCCGGCCCTGGAGCGCGTGCGCATGGAGGCCAAGCGCGTGCCGCCGCCCCTCAACACCATGTAC
GAGGATCTGGCATCGTCCACGTCGGCCATGGTAGGCCGCGACGTGCGTGCCACCGTGGGCAGCAACCTCAATGCCACCAT
CGGGACGTTCTGCCGCAGGGCGATCGGCGGCCGCTATCCCTTCACCAAGGGATCGGCCAGCGACGTGACGAGCGACGACT
TCGCCAGCCTGTTCTCGCCGGGCGGCATGATGGACGAGTTCTTCCGCAACACCCTGCAGCCGATGGTGGACATCTCCACC
ACCCCCTGGCGCTTCCGCCAGGGTGTCGATGGGACGCCGGTCGGTGGATCGGCCGCGCTGGCCTCGTTCCAGCGCGCCGC
GACCATCCGCGACGTGTATTTCCGGGCGGGCGGCAAGGTGCCGTCCATCCGCATGGACATCAAGCCGCTGGAAATGGACG
CCTCCATTTCGCAGATGGTGCTGGACATCGACGGCGAGGTGCTGCGCTACCAGCACGGCCCGCAGATCCCGAAATCGATG
GCCTGGCCCGGCACCCGGGGCACGGGCCAGGTCCGCCTGCAGATCACCGGGGGCGGTGCCGAGAACACGGGCCTGGTGAC
CGAGGGACCCTGGGCCGTGCACCGTTTCTTCGACAAGGCCCAGATTCTGCCGGGCAGCACGCCCGAGCGCTTCGTCGCCG
CCTTCGATATCGGCGGGCGCAAGCTGCGCTTCGAGGTCACCACGGGCAGCGTGCTCAACCCGTTCCGTCTCAAGGCCATG
GAGGAGTTCGCGTGTCCTGGCAATCTCTGA