SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001605
Component protein type	TssJ
T6SS ID (Type)	T6SS00164 (Type i3)
Strain	Acidovorax citrulli AAC00-1
Replicon	chromosome
Sequence	Protein sequence (188 a.a.); Nucleotide sequence (564 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Tian Y, Zhao Y, Wu X, et al. The type VI protein secretion system contributes to biofilm formation and seed-to-seedling transmission of Acidovorax citrulli on melon. Mol Plant Pathol. 2015 Jan;16(1):38-47. doi: 10.1111/mpp.12159. Epub 2014 Jul 16. PMID: 24863458

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AAVE_RS07305
Coordinate (Strand)	1624635..1625198 (+)
NCBI ID	WP_011794611.1
RefSeq	NC_008752
Uniprot ID	A1TM73_ACIAC, A0A6B0DUP1_ACIAI
KEGG ID	aav:Aave_1470
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.2e-33	48..153

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-11	MHREAMARKGF
Transmembrane helix	12-34	IRAGLAAAAAAWLLGGCGMISNL
Outside	35-187	NRPQEAPTAVPPAVFEIVADPGVNPDIHGTPKPILLRIYELRAAAAFDRASYLDLQDKDEAQLGADFVRREEILLQPGERRTIERKGSADVRTFAVFAGYRDLERSTWRATAGAPNSVEMRRRWWGLGETERLKPVQYRITVGKDAVKIQTAP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.7526	pos: 27-28. LGG-CG.	MHREAMARKGFIRAGLAAAAAAWLLGG

Protein sequence: 188 a.a.

						>T6CP001605 NC_008752:1624635-1625198 [Acidovorax citrulli AAC00-1] [TssJ]
MHREAMARKGFIRAGLAAAAAAWLLGGCGMISNLNRPQEAPTAVPPAVFEIVADPGVNPDIHGTPKPILLRIYELRAAAA
FDRASYLDLQDKDEAQLGADFVRREEILLQPGERRTIERKGSADVRTFAVFAGYRDLERSTWRATAGAPNSVEMRRRWWG
LGETERLKPVQYRITVGKDAVKIQTAP*					

Nucleotide sequence: 564 bp

						>T6CP001605 NC_008752:1624635-1625198 [Acidovorax citrulli AAC00-1] [TssJ]
ATGCATCGGGAAGCGATGGCACGGAAGGGATTCATCAGGGCGGGGCTCGCGGCCGCGGCGGCGGCATGGCTGCTGGGCGG
CTGCGGCATGATCAGCAACCTGAACCGCCCGCAGGAGGCCCCCACGGCCGTGCCGCCGGCCGTGTTCGAAATCGTCGCGG
ACCCTGGCGTCAACCCCGATATCCACGGCACGCCCAAGCCCATCCTGCTGAGGATTTACGAACTGCGCGCGGCGGCGGCT
TTCGACCGGGCAAGCTACCTGGACCTGCAGGACAAGGACGAAGCCCAGCTGGGGGCCGATTTCGTGCGGCGCGAGGAGAT
CCTGCTGCAGCCGGGCGAGCGGCGCACCATCGAGCGCAAGGGCAGTGCGGATGTCCGCACCTTCGCCGTGTTCGCAGGCT
ACCGCGACCTGGAGCGCAGCACCTGGCGCGCGACCGCGGGCGCTCCCAACTCCGTGGAGATGCGCCGCCGCTGGTGGGGC
CTGGGCGAAACCGAGCGGCTCAAGCCCGTGCAGTACCGCATCACAGTGGGCAAGGATGCGGTGAAGATCCAGACCGCTCC
TTAA					