SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001584
Component protein type	TssG
T6SS ID (Type)	T6SS00161 (Type i3)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (348 a.a.); Nucleotide sequence (1044 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS19580
Coordinate (Strand)	4279622..4280665 (+)
NCBI ID	WP_011767624.1
RefSeq	NC_008702
Uniprot ID	A1KCG2_AZOSB
KEGG ID	azo:azo3902
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	9.3e-117	17..314

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-347	MRQAHDPVSLFDALAREPWAFDFLQAMRRIECAFPERPRWGSALRPQDEPVRLGQAPSLSFAPATLSAFKTVEGAPPRLEVRFFGLLGPNGPLPLHLTEYAHHRELHHGDETFARFLDLIHHRFLALFYRAWAQAQPTVSLDRPRQDRFSTYIGSLIGIGQPALQQRDSVPDNARRFQAGLLARQVRNGEGLQAVLTGYFELPVAVEQYVGHWMTLRERDHSRLGRARLGSDTVLGRRVWDRQSKFRLQIGPLTLVQYEAFLPGGSALPRLADWVRFYTGGELSWDARLQLQRDQVPPLRLARGARLGWTSWLGTRRGNRDADELVLDAERLVARTPPPRAGDSPHD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9811	-	-

Protein sequence: 348 a.a.

						>T6CP001584 NC_008702:4279622-4280665 [Azoarcus olearius] [TssG]
MRQAHDPVSLFDALAREPWAFDFLQAMRRIECAFPERPRWGSALRPQDEPVRLGQAPSLSFAPATLSAFKTVEGAPPRLE
VRFFGLLGPNGPLPLHLTEYAHHRELHHGDETFARFLDLIHHRFLALFYRAWAQAQPTVSLDRPRQDRFSTYIGSLIGIG
QPALQQRDSVPDNARRFQAGLLARQVRNGEGLQAVLTGYFELPVAVEQYVGHWMTLRERDHSRLGRARLGSDTVLGRRVW
DRQSKFRLQIGPLTLVQYEAFLPGGSALPRLADWVRFYTGGELSWDARLQLQRDQVPPLRLARGARLGWTSWLGTRRGNR
DADELVLDAERLVARTPPPRAGDSPHD*					

Nucleotide sequence: 1044 bp

						>T6CP001584 NC_008702:4279622-4280665 [Azoarcus olearius] [TssG]
ATGCGGCAAGCGCACGATCCTGTAAGCCTGTTCGACGCCCTGGCGCGCGAGCCCTGGGCCTTCGACTTCCTGCAGGCGAT
GCGCCGCATCGAATGCGCCTTTCCCGAGCGGCCGCGCTGGGGCAGCGCGCTGCGCCCGCAGGATGAGCCGGTGCGCCTCG
GGCAGGCGCCCTCGCTGTCGTTCGCGCCGGCCACGCTGTCCGCGTTCAAGACCGTCGAGGGAGCGCCGCCACGGCTGGAG
GTGCGCTTCTTCGGCCTGCTCGGCCCCAACGGCCCGCTGCCGCTGCATCTCACCGAATACGCCCACCACCGCGAGCTGCA
CCACGGCGACGAGACCTTCGCGCGCTTTCTCGACCTCATCCACCACCGCTTCCTTGCGCTGTTCTACCGCGCCTGGGCGC
AGGCCCAGCCCACGGTCAGCCTCGACCGCCCGCGCCAGGACCGCTTCTCCACCTACATCGGCTCGCTGATCGGCATCGGC
CAGCCCGCCCTGCAGCAGCGCGACAGCGTGCCGGACAACGCCCGCCGCTTCCAGGCCGGCCTGCTCGCGCGCCAGGTGCG
CAACGGCGAGGGGCTGCAGGCGGTGCTCACGGGCTATTTCGAGCTGCCGGTGGCGGTGGAACAGTACGTCGGCCACTGGA
TGACGCTGCGCGAACGCGACCATTCGCGCCTGGGTCGCGCCCGGCTGGGCAGCGACACCGTGCTCGGACGCCGGGTGTGG
GACCGCCAGAGCAAGTTCCGCCTGCAGATCGGCCCACTGACGCTGGTCCAGTACGAAGCCTTCCTGCCCGGTGGCAGCGC
GCTGCCGCGGCTGGCGGACTGGGTACGCTTCTACACCGGCGGCGAGCTGTCGTGGGATGCGCGGCTGCAGCTGCAGCGCG
ACCAGGTGCCGCCGCTGCGGCTTGCGCGCGGCGCGCGGCTCGGCTGGACGAGCTGGCTCGGCACCCGGCGCGGCAACCGC
GACGCCGACGAACTGGTGCTCGACGCCGAGCGCCTGGTTGCACGAACACCCCCTCCCCGTGCGGGCGATTCCCCGCACGA
CTGA					