SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001583
Component protein type	TssF
T6SS ID (Type)	T6SS00161 (Type i3)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (624 a.a.); Nucleotide sequence (1872 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS19575
Coordinate (Strand)	4277775..4279646 (+)
NCBI ID	WP_011767623.1
RefSeq	NC_008702
Uniprot ID	A1KCG1_AZOSB
KEGG ID	azo:azo3901
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.8e-230	1..620

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-623	MDPRLLRYYNLELQHLREMGAEFARQFPKIAARLGMDGIEVADPYVERLLEGAGFLAARVQLKLDAEFPRFTQRLLEMLHPHYLAPTPAMLVAQFRPVPGETNLARGFTLPRGTPFRSPLGKGDAAACEFRTAHEIRLWPLELVQARYFSFAADLPLARLPLAGRVRGGVRLTLRTTGGLKFSQLSLDTLNLHLGGAQEVAYRLCELIGAASLGALVQPAGGKGGWQRFIPSEQIRLTGYDDDQALLPTAHRSFGGYRLVQEYFSFPERFLFFDVGGLAPALAQADDEIELVLLFGQGDAVLESVVDNANFLLHCTPAINLFPKRTDRIHVNDSGNEFHVVADRTRPMDYEIYELTEVLGHFHGSRDEQRFLPFYADFHSGTGDSQAYYSCRREPRLLSEKQKKNGPRTGYVGSEVFLSLVDPREAPYRADLRQLSIQALCTNRDLPILLPQGGGQTDFSLDIAAPVEAIRCVRGPSRPYGMLAEGASAWRLISHLALNHFSLVDADAHEGAGALREMLELYATSADVGMRRQVEGLKSVRVLPVVARLPFPGPICFGRGLEIALEVDETAFEGGGAFVFGSVMEHFLARHASLNSFTQTTLRSLTRGVVMRWEPRCGKRTIL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9965	-	-

Protein sequence: 624 a.a.

						>T6CP001583 NC_008702:4277775-4279646 [Azoarcus olearius] [TssF]
MDPRLLRYYNLELQHLREMGAEFARQFPKIAARLGMDGIEVADPYVERLLEGAGFLAARVQLKLDAEFPRFTQRLLEMLH
PHYLAPTPAMLVAQFRPVPGETNLARGFTLPRGTPFRSPLGKGDAAACEFRTAHEIRLWPLELVQARYFSFAADLPLARL
PLAGRVRGGVRLTLRTTGGLKFSQLSLDTLNLHLGGAQEVAYRLCELIGAASLGALVQPAGGKGGWQRFIPSEQIRLTGY
DDDQALLPTAHRSFGGYRLVQEYFSFPERFLFFDVGGLAPALAQADDEIELVLLFGQGDAVLESVVDNANFLLHCTPAIN
LFPKRTDRIHVNDSGNEFHVVADRTRPMDYEIYELTEVLGHFHGSRDEQRFLPFYADFHSGTGDSQAYYSCRREPRLLSE
KQKKNGPRTGYVGSEVFLSLVDPREAPYRADLRQLSIQALCTNRDLPILLPQGGGQTDFSLDIAAPVEAIRCVRGPSRPY
GMLAEGASAWRLISHLALNHFSLVDADAHEGAGALREMLELYATSADVGMRRQVEGLKSVRVLPVVARLPFPGPICFGRG
LEIALEVDETAFEGGGAFVFGSVMEHFLARHASLNSFTQTTLRSLTRGVVMRWEPRCGKRTIL*					

Nucleotide sequence: 1872 bp

						>T6CP001583 NC_008702:4277775-4279646 [Azoarcus olearius] [TssF]
ATGGATCCCCGGCTGCTGCGCTACTACAACCTCGAACTCCAGCACCTGCGCGAGATGGGCGCGGAGTTCGCGCGCCAGTT
TCCCAAGATCGCCGCGCGCCTCGGCATGGACGGCATCGAGGTCGCCGACCCCTATGTCGAGCGCCTGCTCGAAGGCGCGG
GTTTCCTGGCCGCGCGCGTGCAGCTCAAGCTCGACGCCGAATTCCCCCGCTTCACCCAGCGCCTGCTGGAGATGCTGCAC
CCGCACTACCTCGCGCCCACGCCGGCGATGCTGGTGGCGCAGTTCCGCCCGGTGCCGGGCGAGACCAACCTTGCGCGCGG
CTTCACGCTGCCGCGCGGCACGCCGTTCCGCAGCCCGCTCGGCAAGGGCGACGCCGCCGCGTGCGAATTCCGCACCGCGC
ACGAGATCCGGCTGTGGCCGCTGGAGTTGGTGCAGGCGCGCTACTTCAGCTTTGCCGCCGATCTTCCGCTGGCCCGCCTG
CCGCTCGCGGGGCGGGTACGCGGTGGGGTGCGGCTGACGCTGCGCACCACCGGCGGACTCAAGTTCAGCCAGCTCTCGCT
CGACACGCTGAACCTGCACCTCGGCGGCGCGCAGGAAGTGGCCTACCGGCTGTGCGAGCTGATCGGCGCGGCCTCGCTCG
GCGCGCTGGTGCAGCCCGCGGGCGGCAAGGGCGGCTGGCAGCGCTTCATCCCGTCGGAGCAGATCCGCCTGACCGGCTAC
GACGACGATCAGGCCCTGCTGCCCACTGCGCACCGCAGCTTCGGCGGCTACCGGCTGGTACAGGAGTACTTCTCCTTCCC
CGAACGCTTCCTGTTCTTCGACGTTGGCGGGCTGGCCCCCGCGCTGGCCCAGGCCGACGACGAGATCGAACTGGTGCTGC
TGTTCGGCCAGGGCGACGCGGTGCTCGAAAGCGTGGTGGACAACGCCAACTTCCTGCTCCACTGCACGCCGGCAATCAAC
CTGTTTCCGAAGCGCACCGACCGCATCCACGTCAACGACAGCGGCAACGAGTTCCACGTCGTCGCCGACCGCACGCGGCC
GATGGACTACGAAATCTACGAGCTGACCGAAGTGCTCGGTCACTTCCACGGCTCGCGCGACGAACAGCGCTTCCTGCCGT
TCTACGCCGACTTCCATTCCGGCACCGGGGACAGCCAGGCCTACTACAGCTGCCGGCGCGAACCGCGGCTGCTGTCGGAA
AAGCAGAAGAAGAACGGCCCGCGCACCGGCTATGTGGGCAGCGAAGTCTTCCTGTCGCTGGTCGACCCGCGCGAGGCGCC
CTACCGCGCCGACCTGCGCCAGCTGTCGATCCAGGCGCTGTGCACCAACCGCGACCTGCCCATCCTGCTGCCACAGGGTG
GTGGCCAGACCGACTTCTCGCTCGACATCGCCGCGCCGGTCGAGGCGATCCGCTGCGTGCGCGGCCCCTCGCGGCCCTAC
GGCATGCTGGCCGAAGGCGCCAGCGCATGGCGCCTGATCAGCCACCTGGCGCTCAACCACTTCTCGCTGGTGGATGCCGA
CGCGCACGAAGGCGCCGGCGCGCTGCGCGAAATGCTCGAACTCTACGCAACCAGCGCCGACGTCGGCATGCGGCGCCAGG
TGGAAGGGCTGAAGTCGGTCCGCGTGCTTCCCGTCGTCGCCCGCCTGCCCTTCCCCGGGCCGATCTGCTTCGGGCGCGGG
CTGGAGATCGCGCTGGAAGTGGACGAGACCGCGTTCGAGGGCGGCGGCGCCTTCGTGTTCGGCTCGGTCATGGAGCATTT
CCTCGCCCGCCACGCCTCGCTCAACAGCTTCACCCAGACCACGCTGCGCTCGCTCACGCGCGGGGTGGTCATGCGCTGGG
AGCCGCGATGCGGCAAGCGCACGATCCTGTAA					