SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001577
Component protein type	TssA
T6SS ID (Type)	T6SS00161 (Type i3)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (354 a.a.); Nucleotide sequence (1062 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS19540
Coordinate (Strand)	4271852..4272913 (+)
NCBI ID	WP_011767616.1
RefSeq	NC_008702
Uniprot ID	A1KCF4_AZOSB
KEGG ID	azo:azo3894
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.6e-37	11..139

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-353	MSLELDLDTLLAPLGDDQPCGPNLEYDADFLQMEEAARGQAGQEFASDSGSKVSIDGQEADWPAVRRLAEGLLERTRDVRVAVYYTRALLRTEGYPGIHAGLRLIVGLLEQHWAGVHPELDADDNDDPTMRVNALAPLVANDAVIGDLRASTLLRSRQSGVLTVRNIEVTQGKLSARDGEQVLAESQVAGMLAEAVAADPTLADAITDSVALARQLSGFLQDQVGAGASIDFKPLQNVLYAVQQALGGVALPAADGPGEAAAGEEGAPAGVPAAGTPAAPGEIRSRADVVATIDRLVTYLERSEPTNPAQWLLRRAQRVMNMNFLEAIVELAPDGLEQAERMVGGQLNPEQDD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9861	-	-

Protein sequence: 354 a.a.

						>T6CP001577 NC_008702:4271852-4272913 [Azoarcus olearius] [TssA]
MSLELDLDTLLAPLGDDQPCGPNLEYDADFLQMEEAARGQAGQEFASDSGSKVSIDGQEADWPAVRRLAEGLLERTRDVR
VAVYYTRALLRTEGYPGIHAGLRLIVGLLEQHWAGVHPELDADDNDDPTMRVNALAPLVANDAVIGDLRASTLLRSRQSG
VLTVRNIEVTQGKLSARDGEQVLAESQVAGMLAEAVAADPTLADAITDSVALARQLSGFLQDQVGAGASIDFKPLQNVLY
AVQQALGGVALPAADGPGEAAAGEEGAPAGVPAAGTPAAPGEIRSRADVVATIDRLVTYLERSEPTNPAQWLLRRAQRVM
NMNFLEAIVELAPDGLEQAERMVGGQLNPEQDD*					

Nucleotide sequence: 1062 bp

						>T6CP001577 NC_008702:4271852-4272913 [Azoarcus olearius] [TssA]
ATGTCGCTGGAACTCGATCTCGACACACTGCTGGCACCGCTGGGCGATGACCAACCCTGCGGCCCCAACCTCGAATACGA
CGCCGACTTCCTCCAGATGGAAGAGGCCGCGCGCGGGCAAGCCGGCCAGGAGTTCGCGAGCGACAGCGGCAGCAAGGTCA
GCATCGATGGCCAGGAAGCGGACTGGCCCGCGGTGCGCCGGCTCGCCGAGGGCCTCCTCGAGCGCACCCGGGACGTCCGC
GTCGCGGTCTATTACACCCGCGCGCTGCTGCGCACCGAAGGCTATCCCGGCATCCACGCGGGACTCCGCCTGATCGTCGG
CCTGCTCGAACAGCACTGGGCCGGCGTCCACCCCGAACTCGACGCCGACGACAACGACGACCCGACGATGCGGGTGAATG
CGCTGGCGCCGCTGGTTGCCAACGACGCGGTGATCGGCGACCTGCGCGCCAGCACGCTGTTGCGCTCGCGCCAGTCCGGC
GTGCTGACGGTGCGCAACATCGAGGTCACCCAGGGCAAGCTCAGCGCGCGCGACGGCGAACAGGTGCTGGCCGAGTCCCA
GGTGGCCGGCATGCTGGCCGAAGCGGTCGCGGCCGATCCCACCCTGGCCGACGCGATCACCGACAGCGTCGCGCTCGCCC
GCCAGCTCTCCGGCTTCCTGCAGGATCAGGTGGGCGCCGGCGCCAGCATCGATTTCAAGCCGCTGCAGAACGTCCTTTAC
GCCGTGCAGCAGGCGCTCGGCGGCGTTGCGCTGCCCGCTGCCGACGGCCCGGGCGAGGCCGCGGCCGGTGAGGAAGGCGC
CCCGGCCGGCGTGCCGGCCGCCGGCACGCCCGCGGCCCCGGGCGAAATCCGCTCGCGCGCCGACGTCGTCGCCACCATCG
ACCGCCTGGTGACCTACCTCGAACGCAGCGAACCGACCAACCCCGCGCAGTGGCTGCTGCGGCGTGCCCAGCGGGTGATG
AACATGAACTTCCTCGAAGCCATCGTCGAACTTGCCCCCGATGGTCTCGAACAAGCCGAGCGCATGGTCGGCGGCCAGCT
CAACCCCGAGCAGGACGACTAG					