SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001576
Component protein type	TssJ
T6SS ID (Type)	T6SS00161 (Type i3)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS19535
Coordinate (Strand)	4271065..4271583 (-)
NCBI ID	WP_011767615.1
RefSeq	NC_008702
Uniprot ID	A1KCF3_AZOSB
KEGG ID	aoa:dqs_4037, azo:azo3893
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.6e-36	38..153

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MNVRASALRVVLPAVAAAVLAACSKPVVAPPKPEPTVVTPTLAAAPRINIDTRGRPTPVVLRVYLLKNASAFEGADFFSLFDREQQVLGDAVLAREEIVLKPGETRTLDARDAEGARVVGVLAAFREVDRAVWRATVPVVPNRGNLVTIRVEDDRISLAATLAPLPPPKKAD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9947	pos: 22-23. LAA-CS.	MNVRASALRVVLPAVAAAVLAA

Protein sequence: 173 a.a.

						>T6CP001576 NC_008702:c4271583-4271065 [Azoarcus olearius] [TssJ]
MNVRASALRVVLPAVAAAVLAACSKPVVAPPKPEPTVVTPTLAAAPRINIDTRGRPTPVVLRVYLLKNASAFEGADFFSL
FDREQQVLGDAVLAREEIVLKPGETRTLDARDAEGARVVGVLAAFREVDRAVWRATVPVVPNRGNLVTIRVEDDRISLAA
TLAPLPPPKKAD*					

Nucleotide sequence: 519 bp

						>T6CP001576 NC_008702:c4271583-4271065 [Azoarcus olearius] [TssJ]
TTGAATGTTCGCGCCAGCGCGCTGCGTGTCGTGCTGCCGGCGGTCGCCGCGGCGGTGCTCGCGGCCTGCTCCAAGCCGGT
CGTCGCACCGCCCAAGCCCGAGCCGACGGTGGTGACGCCGACGCTCGCGGCCGCTCCCCGCATCAATATAGATACGCGCG
GCCGCCCCACCCCGGTGGTGTTGCGGGTGTATCTGCTGAAGAACGCCTCGGCGTTCGAGGGCGCGGATTTCTTCTCGCTG
TTCGATCGCGAGCAGCAGGTGCTCGGCGACGCGGTGCTGGCGCGCGAGGAGATCGTCCTCAAGCCCGGCGAGACGCGCAC
GCTGGATGCCCGTGATGCCGAGGGCGCCCGCGTGGTCGGCGTGCTCGCCGCGTTCCGCGAGGTGGACCGCGCGGTGTGGC
GCGCCACCGTGCCCGTCGTTCCCAATCGCGGCAACCTGGTGACGATCCGCGTCGAGGACGACCGCATCAGCCTCGCCGCC
ACCCTGGCGCCGTTGCCGCCGCCCAAGAAAGCCGACTAA					