Detailed information of component protein


Summary

Component ID T6CP001574
Component protein type TssL
T6SS ID (Type) T6SS00161 experimental (Type i3)
Strain Azoarcus olearius
Replicon chromosome
Sequence Protein sequence (435 a.a.); Nucleotide sequence (1305 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AZO_RS19525
Coordinate (Strand) 4268313..4269617 (-)
NCBI ID WP_011767613.1
RefSeq NC_008702
Uniprot ID A1KCF1_AZOSB
KEGG ID azo:azo3891
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.1e-69 56..256
OmpA PF00691.22 7.3e-13 325..422
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-235 MNQDDPFGQPPGDRTLVIPNPGARAARPAAAAAPAQGFSFGRVELSALDWSAGLNPLVAAANPLLNLVPQLRQAHHPDPAGLRETLARAVQAFETKAREVGVLNEHVVGARYALCTFLDETAANTPWGEKIWAQKSLLVQFHNEAWGGEKFFQLLGKVAEQPGTHRNLLELFYIILALGFEGRYRVLQNGRAQLDAVRERLAQMILKERGEPVRELSPQWQTTATSARPLRDSVP
Transmembrane helix 236-258 IWAVAAIAALLLVLIYLAASYAL
Inside 259-434 NRHSDPAFAQILGLKVPPPAPLAQAAAVPAKVPDRLQGFLAPEIREGLVTVRDLPDRSVVQIQGDGLFEPASATLAPRVLPLMARIGEAVARVKGSVLVRGHSDDRPIRSARFPSNWHLSQARADSVAAVLAPQLGGARIETEGRAESEPVASNDTPEGRARNRRVEIIVFPAAGD


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8790 - -


  Protein sequence: 435 a.a.    

>T6CP001574 NC_008702:c4269617-4268313 [Azoarcus olearius] [TssL]
MNQDDPFGQPPGDRTLVIPNPGARAARPAAAAAPAQGFSFGRVELSALDWSAGLNPLVAAANPLLNLVPQLRQAHHPDPA
GLRETLARAVQAFETKAREVGVLNEHVVGARYALCTFLDETAANTPWGEKIWAQKSLLVQFHNEAWGGEKFFQLLGKVAE
QPGTHRNLLELFYIILALGFEGRYRVLQNGRAQLDAVRERLAQMILKERGEPVRELSPQWQTTATSARPLRDSVPIWAVA
AIAALLLVLIYLAASYALNRHSDPAFAQILGLKVPPPAPLAQAAAVPAKVPDRLQGFLAPEIREGLVTVRDLPDRSVVQI
QGDGLFEPASATLAPRVLPLMARIGEAVARVKGSVLVRGHSDDRPIRSARFPSNWHLSQARADSVAAVLAPQLGGARIET
EGRAESEPVASNDTPEGRARNRRVEIIVFPAAGD*

  Nucleotide sequence: 1305 bp    

>T6CP001574 NC_008702:c4269617-4268313 [Azoarcus olearius] [TssL]
ATGAACCAAGACGATCCTTTCGGCCAGCCACCCGGCGACCGTACGCTGGTGATTCCCAACCCGGGAGCGCGCGCCGCGCG
CCCGGCGGCGGCCGCGGCGCCGGCGCAGGGCTTCAGCTTCGGGCGGGTTGAGCTGTCCGCGCTCGACTGGAGCGCCGGCC
TCAATCCGCTGGTCGCCGCCGCCAATCCCTTGCTCAACCTGGTGCCGCAGCTGCGTCAGGCCCATCACCCCGATCCCGCC
GGGCTGCGCGAAACGCTCGCGCGCGCGGTGCAGGCCTTCGAAACCAAGGCGCGCGAGGTCGGCGTGCTCAACGAGCATGT
CGTCGGCGCCCGCTACGCGCTGTGCACCTTCCTCGACGAGACCGCCGCCAACACGCCGTGGGGCGAGAAGATCTGGGCGC
AGAAGAGCCTGCTGGTGCAGTTCCACAACGAAGCCTGGGGCGGCGAGAAGTTCTTCCAGCTGCTGGGCAAGGTCGCCGAG
CAGCCCGGCACCCACCGCAACCTGCTCGAACTCTTCTACATCATCCTGGCGCTCGGCTTCGAGGGCCGCTATCGCGTGCT
GCAGAACGGCCGCGCCCAGCTCGACGCGGTGCGCGAACGGCTGGCGCAGATGATCCTCAAGGAGCGCGGCGAGCCGGTGC
GCGAACTGTCACCTCAGTGGCAGACCACCGCGACCAGCGCGCGCCCGCTGCGCGACTCGGTGCCGATCTGGGCGGTCGCG
GCGATTGCCGCGCTGCTGCTGGTGCTGATCTATCTGGCCGCGTCCTACGCGCTCAACCGCCACTCCGACCCCGCTTTCGC
GCAGATCCTCGGCCTCAAGGTGCCGCCGCCGGCGCCGCTGGCACAGGCCGCCGCGGTGCCGGCGAAGGTGCCCGACCGGT
TGCAGGGCTTCCTCGCCCCCGAAATCCGCGAAGGGCTGGTGACCGTGCGCGACCTGCCGGACCGCAGCGTGGTGCAGATC
CAGGGCGACGGCCTGTTCGAACCGGCCAGCGCCACGCTGGCGCCGCGCGTGCTGCCGCTGATGGCGCGCATCGGCGAGGC
GGTGGCGCGGGTCAAGGGTTCGGTGCTGGTGCGCGGCCATTCCGACGACCGTCCGATCCGCTCCGCGCGCTTCCCGTCCA
ACTGGCATCTGTCGCAGGCCCGCGCCGATTCGGTGGCGGCGGTGCTGGCGCCGCAGCTCGGCGGCGCGCGCATCGAGACC
GAGGGCCGCGCCGAGAGCGAGCCGGTTGCCTCCAACGACACCCCGGAAGGGCGCGCGCGCAACCGCCGCGTCGAGATCAT
CGTGTTCCCGGCCGCGGGCGACTGA