SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001572
Component protein type	TssI
T6SS ID (Type)	T6SS00161 (Type i3)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (691 a.a.); Nucleotide sequence (2073 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS19450
Coordinate (Strand)	4249321..4251393 (+)
NCBI ID	WP_011767598.1
RefSeq	NC_008702
Uniprot ID	A1KCD6_AZOSB
KEGG ID	azo:azo3876
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	4.8e-105	27..326
Phage_base_V	PF04717.14	2e-15	382..450
Gp5_C	PF06715.14	2.8e-05	558..581
Gp5_C	PF06715.14	9.8e-05	535..557

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-690	VADQSQFELITPLGSGVLVFQALAADEALSQVSEFEIDCLSDKTDINLDDILGKQLSLRVMLPTGGKRYFSGYVTRFAHTGTQGRYQTYRASVRPWLWFLGRTADCRIFQSRTVPDIVKQVFSEHGVADFRNALSGSYRTREYCVQYRETDLAFVSRLLEEEGIYYFFEHDENKNTLVLADAYAAHSSTAGYEEIEFVENRQGGGDELGFVSQWQFERQVMPGKVMLRDYDFTKPSVGLEASAVVPREHDEGKHEVFDYPGEFDQVADGRHYAKVRVDELHAQFELSRARSSARGLAVGHLFKLAGHARRDQNREYLITAAQTRVRAEGRESEAGAGSQFECHFSALNSRQDYRPPRLTPRPQVKGPQTAVVVGPSGNEIYTDEYGRVKVQFHWDRYGTKDEHSSCWIRVSHPWAGKNWGFIAIPRIGQEVIVEFLEGDPDQPIITGRVYNAEQMPPYALPDNMTQTGIKTRSTLGGGTDNFNEIRFEDKKGEEQLFIHAEKNQDIEVENDETHWVGHDRTKNIDHNETVTVGNDRTESVGNNETISIGNNRTESVGANETISVGGNRAEDVGQNETVSIGKDRSESIGNNDAHDVAKNQTIAIGENQTLEVGKARSTTVGDDDKLQVGKKLVIEAGDEITIKTGSASITMKKDGTIQIKGKDITIKGDGQIGIKAASDVVIKGAKISEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.6980	-	-

Protein sequence: 691 a.a.

						>T6CP001572 NC_008702:4249321-4251393 [Azoarcus olearius] [TssI]
VADQSQFELITPLGSGVLVFQALAADEALSQVSEFEIDCLSDKTDINLDDILGKQLSLRVMLPTGGKRYFSGYVTRFAHT
GTQGRYQTYRASVRPWLWFLGRTADCRIFQSRTVPDIVKQVFSEHGVADFRNALSGSYRTREYCVQYRETDLAFVSRLLE
EEGIYYFFEHDENKNTLVLADAYAAHSSTAGYEEIEFVENRQGGGDELGFVSQWQFERQVMPGKVMLRDYDFTKPSVGLE
ASAVVPREHDEGKHEVFDYPGEFDQVADGRHYAKVRVDELHAQFELSRARSSARGLAVGHLFKLAGHARRDQNREYLITA
AQTRVRAEGRESEAGAGSQFECHFSALNSRQDYRPPRLTPRPQVKGPQTAVVVGPSGNEIYTDEYGRVKVQFHWDRYGTK
DEHSSCWIRVSHPWAGKNWGFIAIPRIGQEVIVEFLEGDPDQPIITGRVYNAEQMPPYALPDNMTQTGIKTRSTLGGGTD
NFNEIRFEDKKGEEQLFIHAEKNQDIEVENDETHWVGHDRTKNIDHNETVTVGNDRTESVGNNETISIGNNRTESVGANE
TISVGGNRAEDVGQNETVSIGKDRSESIGNNDAHDVAKNQTIAIGENQTLEVGKARSTTVGDDDKLQVGKKLVIEAGDEI
TIKTGSASITMKKDGTIQIKGKDITIKGDGQIGIKAASDVVIKGAKISEN*					

Nucleotide sequence: 2073 bp

						>T6CP001572 NC_008702:4249321-4251393 [Azoarcus olearius] [TssI]
GTGGCAGATCAATCGCAATTCGAACTCATCACCCCGCTGGGCAGCGGCGTGCTCGTATTCCAGGCGCTCGCCGCCGACGA
GGCGCTGTCGCAGGTCAGCGAATTCGAGATCGACTGCCTCTCCGACAAGACCGACATCAACCTCGACGACATCCTCGGCA
AGCAGCTCAGCCTGCGGGTGATGCTGCCCACCGGCGGCAAGCGCTACTTCAGCGGCTACGTCACCCGCTTCGCCCACACC
GGCACCCAGGGCCGCTACCAGACCTACCGCGCGAGCGTGCGGCCGTGGCTGTGGTTCCTCGGCCGCACCGCCGACTGCCG
CATCTTCCAGTCGCGCACGGTGCCCGACATCGTCAAGCAGGTCTTCTCCGAACACGGCGTCGCCGACTTCCGCAACGCGC
TCAGCGGCAGCTACCGCACGCGCGAATACTGCGTGCAGTACCGCGAGACCGACCTCGCCTTCGTCAGCCGGCTGCTGGAA
GAAGAGGGCATCTATTACTTCTTCGAGCACGACGAGAACAAGAACACGCTGGTGCTGGCCGACGCCTACGCCGCGCACAG
CAGCACCGCCGGCTACGAGGAGATCGAGTTCGTCGAGAACCGCCAGGGCGGCGGCGACGAGCTGGGCTTCGTCAGCCAGT
GGCAGTTCGAGCGCCAGGTGATGCCGGGCAAGGTCATGCTGCGCGACTACGACTTCACCAAGCCCTCGGTCGGGCTGGAA
GCCTCGGCGGTGGTGCCGCGCGAACACGACGAGGGCAAGCACGAGGTGTTCGACTACCCCGGCGAGTTCGACCAGGTGGC
CGACGGCCGCCACTACGCCAAGGTGCGCGTCGATGAGCTGCACGCGCAGTTCGAACTGTCCCGCGCGCGCAGCAGCGCCC
GCGGCCTGGCGGTGGGCCACCTGTTCAAGCTCGCCGGTCACGCCCGCCGCGACCAGAACCGCGAGTACCTGATCACCGCG
GCGCAGACCCGCGTGCGTGCCGAAGGGCGGGAATCCGAAGCGGGTGCGGGCAGCCAGTTCGAGTGCCACTTCAGCGCCCT
CAACAGCCGCCAGGATTACCGCCCGCCGCGGCTGACCCCGCGCCCGCAGGTGAAGGGGCCGCAGACCGCGGTAGTGGTCG
GCCCGTCCGGCAACGAGATCTACACCGACGAATACGGCCGCGTGAAGGTGCAGTTCCACTGGGACCGCTACGGCACCAAG
GACGAGCACAGCTCGTGCTGGATCCGCGTCTCCCATCCCTGGGCGGGCAAGAACTGGGGCTTCATCGCCATCCCCCGCAT
CGGCCAGGAGGTGATCGTCGAATTCCTCGAGGGCGATCCCGACCAGCCGATCATCACCGGCCGGGTCTACAACGCCGAGC
AGATGCCGCCATACGCGCTGCCCGACAACATGACCCAGACCGGCATCAAGACGCGCTCCACGCTCGGCGGCGGCACCGAC
AACTTCAACGAGATCCGCTTCGAGGACAAGAAGGGCGAGGAGCAGCTCTTCATCCACGCCGAGAAGAACCAGGACATCGA
GGTCGAGAACGACGAGACCCACTGGGTCGGCCACGACCGCACCAAGAACATCGACCACAACGAGACGGTCACCGTGGGCA
ACGACCGCACCGAGTCGGTCGGCAACAACGAAACCATCAGCATCGGCAACAACCGCACCGAATCGGTGGGCGCCAACGAA
ACCATCTCGGTGGGCGGCAACCGCGCCGAGGACGTCGGCCAGAACGAAACCGTCAGCATCGGCAAGGACCGCAGCGAGAG
CATCGGCAACAACGACGCCCACGACGTCGCCAAGAACCAGACCATCGCGATCGGCGAAAACCAGACGCTGGAGGTGGGCA
AGGCGCGCTCCACCACGGTCGGCGATGACGACAAGCTGCAGGTCGGCAAGAAACTGGTGATCGAGGCCGGCGACGAGATC
ACCATCAAGACCGGCAGCGCCAGCATCACCATGAAGAAGGACGGCACGATCCAGATCAAGGGCAAGGACATCACCATCAA
GGGCGACGGCCAGATCGGCATCAAGGCCGCTTCCGACGTGGTGATCAAGGGCGCCAAGATCTCCGAGAACTGA					