SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001569
Component protein type	TssF
T6SS ID (Type)	T6SS00160 (Type i4b)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (613 a.a.); Nucleotide sequence (1839 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS06630
Coordinate (Strand)	1426635..1428473 (+)
NCBI ID	WP_011765041.1
RefSeq	NC_008702
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.1e-211	1..608

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-612	MFDELLRYYETELGYLRELSGEFARRYPKIAGRLQLEGDQCEDPHVERLIEAFAFLGARIHRRLDDDLPEITASLLDALYPHFLQPVPSASIVQFELDPARPEVAGRYRIERGQMLLSPPVDDLACRFRTCYPVELYPLTVASARVVFTAVSAHLAAQAPDATAVLTLSLETQGGVDLGALQLDRLRFFLDGEPALTYLIHQLLLQHEAQPWVEDQGGRLQRLPRGALRPVGFARDEGLLAYDDRSFLGYRLLTEYFTLPEKFLFVDVTGLPALSGQRFRLHCPIRRYPGAERHTRLLESVEARHFRLGCTPVINLFPRAAEPVRVTHRRESYPIVVDARRPLGYEVIAIERVHYVERDDAGTRATEVPPLYGLNGAAEADAARFRWHARRDGSTRANDRGTEVSLHLVDARFSAVRPATEVLSLALLCSNRDLPERLPFGGGDAGPHTDFTLPGQAVVKRVRLLRKPTSSLRNPHRKGFQWRLVSHLSLNYLSIVSQGKGALQDILALYNPLDRPAARRHIEGIREVNSAPAVARLAGPDFISFVRGTGVDLVLDEEAFVGASAYLFASVLERFFALYCAPNSFVQLRYRCLNDEENVVQWPPRSGEAIVI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9968	-	-

Protein sequence: 613 a.a.

						>T6CP001569 NC_008702:1426635-1428473 [Azoarcus olearius] [TssF]
MFDELLRYYETELGYLRELSGEFARRYPKIAGRLQLEGDQCEDPHVERLIEAFAFLGARIHRRLDDDLPEITASLLDALY
PHFLQPVPSASIVQFELDPARPEVAGRYRIERGQMLLSPPVDDLACRFRTCYPVELYPLTVASARVVFTAVSAHLAAQAP
DATAVLTLSLETQGGVDLGALQLDRLRFFLDGEPALTYLIHQLLLQHEAQPWVEDQGGRLQRLPRGALRPVGFARDEGLL
AYDDRSFLGYRLLTEYFTLPEKFLFVDVTGLPALSGQRFRLHCPIRRYPGAERHTRLLESVEARHFRLGCTPVINLFPRA
AEPVRVTHRRESYPIVVDARRPLGYEVIAIERVHYVERDDAGTRATEVPPLYGLNGAAEADAARFRWHARRDGSTRANDR
GTEVSLHLVDARFSAVRPATEVLSLALLCSNRDLPERLPFGGGDAGPHTDFTLPGQAVVKRVRLLRKPTSSLRNPHRKGF
QWRLVSHLSLNYLSIVSQGKGALQDILALYNPLDRPAARRHIEGIREVNSAPAVARLAGPDFISFVRGTGVDLVLDEEAF
VGASAYLFASVLERFFALYCAPNSFVQLRYRCLNDEENVVQWPPRSGEAIVI*					

Nucleotide sequence: 1839 bp

						>T6CP001569 NC_008702:1426635-1428473 [Azoarcus olearius] [TssF]
ATGTTCGACGAACTGTTGCGCTACTACGAAACCGAACTCGGCTACCTGCGCGAACTCTCGGGCGAGTTCGCGCGCCGTTA
CCCGAAGATCGCCGGCCGTCTTCAGCTGGAGGGAGACCAGTGCGAGGACCCGCATGTCGAGCGTCTCATCGAAGCCTTCG
CCTTTCTCGGTGCCCGCATCCATCGCCGCCTGGACGACGACCTGCCGGAAATCACCGCCAGCCTGCTCGACGCCCTGTAT
CCCCACTTTCTGCAACCTGTTCCTTCGGCCAGCATCGTGCAGTTCGAACTCGACCCCGCGCGTCCGGAAGTGGCGGGGCG
CTACCGGATAGAGCGCGGGCAGATGCTGCTGTCGCCACCGGTGGACGACCTGGCCTGCCGGTTTCGCACCTGCTATCCGG
TCGAGCTGTATCCGCTGACGGTCGCGTCGGCCCGGGTGGTCTTCACCGCAGTCAGTGCCCACCTTGCCGCGCAGGCGCCG
GATGCGACCGCCGTGCTGACCTTGTCGCTGGAGACCCAGGGCGGCGTCGATCTCGGTGCGCTCCAGCTCGACCGGCTGCG
CTTCTTCCTCGATGGCGAGCCGGCGCTGACCTATCTGATCCACCAGTTGCTGCTGCAACACGAAGCGCAACCCTGGGTGG
AGGACCAAGGCGGGCGGCTTCAGCGCCTGCCCCGCGGTGCGCTGCGGCCAGTCGGCTTTGCCCGGGACGAGGGGCTGCTC
GCCTATGACGACCGCTCCTTCCTCGGTTACCGCTTGCTGACCGAGTACTTCACGCTGCCGGAGAAGTTCCTGTTCGTGGA
CGTCACCGGCCTGCCGGCGCTGTCGGGGCAGCGTTTTCGGCTGCACTGCCCGATCAGGCGCTATCCAGGGGCAGAGCGCC
ATACCCGTCTGCTCGAAAGCGTCGAGGCGCGGCATTTCCGCCTTGGCTGCACCCCGGTGATCAACCTGTTCCCGCGCGCA
GCCGAACCGGTTCGCGTCACTCATCGGCGCGAGTCCTACCCGATCGTGGTGGACGCCCGCCGCCCGCTCGGTTATGAGGT
GATCGCGATCGAGCGTGTGCACTACGTCGAAAGGGACGACGCCGGCACGCGCGCAACGGAAGTCCCACCGCTCTATGGTT
TGAATGGGGCCGCAGAGGCGGATGCCGCGCGTTTTCGGTGGCATGCCCGGCGGGACGGTTCGACCCGGGCCAACGACAGG
GGAACCGAGGTATCGCTCCATCTGGTGGATGCCCGCTTCTCGGCCGTCCGCCCCGCGACGGAGGTGCTGAGCCTGGCGCT
GTTGTGCAGCAACCGCGACCTGCCGGAGCGACTGCCTTTTGGCGGCGGTGATGCCGGTCCGCACACCGATTTCACTCTGC
CGGGACAGGCGGTGGTGAAGCGGGTGCGGCTGTTGCGCAAGCCCACGTCCAGCCTGCGCAACCCGCACCGCAAGGGCTTC
CAGTGGCGGCTGGTGTCGCACCTGTCGCTCAACTACCTGTCCATCGTCAGCCAGGGCAAGGGGGCGCTGCAGGACATCCT
CGCGCTCTACAACCCGCTCGACCGGCCGGCCGCGCGGCGCCACATCGAAGGTATCCGCGAGGTGAACAGCGCTCCCGCGG
TTGCTCGTCTTGCCGGCCCCGACTTCATCAGTTTCGTCCGTGGCACCGGGGTGGACCTCGTGCTGGACGAAGAGGCCTTT
GTCGGTGCCAGCGCCTACTTGTTCGCCAGCGTGCTCGAACGCTTCTTTGCCTTGTACTGCGCACCCAACAGCTTCGTGCA
ATTGCGCTACCGGTGCCTGAACGATGAGGAGAACGTGGTCCAATGGCCACCCCGCAGCGGCGAAGCGATCGTGATCTGA					