Detailed information of component protein


Summary

Component ID T6CP001564
Component protein type TssM
T6SS ID (Type) T6SS00160 insolico (Type i4b)
Strain Azoarcus olearius
Replicon chromosome
Sequence Protein sequence (1231 a.a.); Nucleotide sequence (3693 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) AZO_RS06600
Coordinate (Strand) 1417056..1420748 (+)
NCBI ID WP_011765035.1
RefSeq NC_008702
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 3.1e-85 476..849
IcmF-related_N PF14331.8 9.1e-57 180..431
IcmF_C PF06744.14 3.9e-21 1106..1207
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MNPRFR
Transmembrane helix 7-29 RLMRISGPALLLLAAVLVLVQTL
Outside 30-38 PPGWDRGGE
Transmembrane helix 39-61 GVVRVLAAFSVVLLAGVALWAFS
Inside 62-424 RRWRMRGATGAAAQARPGPASAEPGSVLRSGLRAAVESIRASDLGRRAGRNALGQLPWYGVIGAEGAGKTALIKQSGLSFPFEHLPGCPDDGDCGWFLATEAVVLDLGGRYLSDSLGDSSWLEFWRELKRLRPAQPLDGLVAAVSAAELLGGDERALHAHATRVRACIDEIQDAVGVRLPVYLVVTQLDLIGQGALPSERDAPRSPFGFAIPAVPADRTIDSVAERFALFRAGLRHHVLAAAEMHDPLTQSEQLSFSVEFDGLGARLNSFVRVLLGNNPYHVQPALRGVYFSSLPAGGGHTVLGPRTDALAQRFELDLRDTRSSGFSHDRQDAPSPARLFHDVVLPDRFFARRLRSASASQGR
Transmembrane helix 425-447 WAWMATSLAVVCLAVGLMTASFT
Outside 448-1230 GNHQLLTELAASQQAAQTSGDGVPLAVELERLVALQHQLERLRRYRLAQPPWRLRFGLYQGDKVEAMLRARYFAGVRQLMLEPVAREIERELGATASAPVRRAGAGRPAGAVTANDRPPAGLPIIPLGEGAAAQPLRAAGIPPGGPALSARVRRSAGESAGIEGQYNALKTYLMLADPSRIEAEHLADQLPRFWRRWLQAERGQTPLPDIYALAQRMVDFYIEERAAPDLPVIAAEGALVARSRSALIGAMRRLPPEERIYRALLAAANVRFEPVSVTGVLRDHASDLVGGSVVVPGAFTRKAYDEYLRSAFGLSGRDLDTGTDWVLDDHAGNLAESSSGAVDPGALERRYREDFEIAWLGFLQGLTVPVFADLAAATDALGQLGDPARSPLLPLLQRAGHELGGRGRAVEGERGRMETVLARARGVLDAGRSEAVADAGPASEASPLPGVVALAGGPVEAGDPRLDGALREYLARLLKLRDRLAVVAAAGSDAEPAIRLVRATFDGRESEFPASLSFVDNELLGAMDSASRTALRPVLTQPVLRAFDALIPLVSRDLDLAWQREVLPHWRRLAETFPFSDSEHDVVPSELAEFAAQGGVLDRFVERRLEGLVSRRGQGLATRNWGDRSIALNPAFLPALERLLAFGAALPRQGELARFELQPMPTPGLTEIVIEIDGQSLRYRNGPQTWASFSWPGTAVQEAAVRVVDVSGAPGAVLHRRGRMALIRLLAEASVQGATPGPLQFAWNGAGAAVRFNFRSLSGASPLDLLALRKLTLPDRISR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9522 - -


  Protein sequence: 1231 a.a.    

>T6CP001564 NC_008702:1417056-1420748 [Azoarcus olearius] [TssM]
MNPRFRRLMRISGPALLLLAAVLVLVQTLPPGWDRGGEGVVRVLAAFSVVLLAGVALWAFSRRWRMRGATGAAAQARPGP
ASAEPGSVLRSGLRAAVESIRASDLGRRAGRNALGQLPWYGVIGAEGAGKTALIKQSGLSFPFEHLPGCPDDGDCGWFLA
TEAVVLDLGGRYLSDSLGDSSWLEFWRELKRLRPAQPLDGLVAAVSAAELLGGDERALHAHATRVRACIDEIQDAVGVRL
PVYLVVTQLDLIGQGALPSERDAPRSPFGFAIPAVPADRTIDSVAERFALFRAGLRHHVLAAAEMHDPLTQSEQLSFSVE
FDGLGARLNSFVRVLLGNNPYHVQPALRGVYFSSLPAGGGHTVLGPRTDALAQRFELDLRDTRSSGFSHDRQDAPSPARL
FHDVVLPDRFFARRLRSASASQGRWAWMATSLAVVCLAVGLMTASFTGNHQLLTELAASQQAAQTSGDGVPLAVELERLV
ALQHQLERLRRYRLAQPPWRLRFGLYQGDKVEAMLRARYFAGVRQLMLEPVAREIERELGATASAPVRRAGAGRPAGAVT
ANDRPPAGLPIIPLGEGAAAQPLRAAGIPPGGPALSARVRRSAGESAGIEGQYNALKTYLMLADPSRIEAEHLADQLPRF
WRRWLQAERGQTPLPDIYALAQRMVDFYIEERAAPDLPVIAAEGALVARSRSALIGAMRRLPPEERIYRALLAAANVRFE
PVSVTGVLRDHASDLVGGSVVVPGAFTRKAYDEYLRSAFGLSGRDLDTGTDWVLDDHAGNLAESSSGAVDPGALERRYRE
DFEIAWLGFLQGLTVPVFADLAAATDALGQLGDPARSPLLPLLQRAGHELGGRGRAVEGERGRMETVLARARGVLDAGRS
EAVADAGPASEASPLPGVVALAGGPVEAGDPRLDGALREYLARLLKLRDRLAVVAAAGSDAEPAIRLVRATFDGRESEFP
ASLSFVDNELLGAMDSASRTALRPVLTQPVLRAFDALIPLVSRDLDLAWQREVLPHWRRLAETFPFSDSEHDVVPSELAE
FAAQGGVLDRFVERRLEGLVSRRGQGLATRNWGDRSIALNPAFLPALERLLAFGAALPRQGELARFELQPMPTPGLTEIV
IEIDGQSLRYRNGPQTWASFSWPGTAVQEAAVRVVDVSGAPGAVLHRRGRMALIRLLAEASVQGATPGPLQFAWNGAGAA
VRFNFRSLSGASPLDLLALRKLTLPDRISR*

  Nucleotide sequence: 3693 bp    

>T6CP001564 NC_008702:1417056-1420748 [Azoarcus olearius] [TssM]
ATGAATCCTCGCTTCAGGCGTTTGATGCGGATTTCCGGGCCGGCGCTGTTGCTCCTGGCGGCGGTGCTGGTGCTTGTCCA
GACCTTGCCGCCCGGATGGGACCGCGGGGGTGAGGGCGTCGTGCGGGTGCTTGCGGCATTTTCGGTGGTGTTGCTGGCAG
GCGTTGCTCTGTGGGCCTTCTCGCGACGCTGGCGCATGCGCGGAGCAACAGGCGCGGCAGCGCAAGCGCGGCCGGGACCC
GCAAGCGCCGAGCCGGGGTCTGTCCTACGCAGTGGTCTCAGGGCCGCTGTCGAATCGATTCGTGCCTCCGACCTGGGGCG
CCGGGCGGGCCGCAATGCGCTCGGCCAGTTGCCCTGGTACGGCGTGATCGGGGCCGAGGGCGCGGGCAAGACCGCTTTGA
TCAAGCAGTCGGGCTTGAGCTTTCCTTTCGAGCACCTTCCCGGCTGCCCTGACGATGGCGATTGCGGCTGGTTTCTGGCG
ACGGAGGCGGTGGTGCTCGACCTTGGCGGGCGCTACCTGTCGGACTCGCTTGGCGATTCGTCGTGGTTGGAGTTCTGGCG
TGAGTTGAAGCGGTTGCGTCCTGCCCAGCCGTTGGACGGCCTCGTCGCCGCTGTCAGCGCAGCGGAGCTGCTGGGCGGCG
ACGAGCGGGCGCTCCACGCGCATGCCACCCGAGTGCGGGCCTGCATCGATGAAATCCAGGATGCGGTAGGGGTGCGCCTG
CCGGTGTATCTCGTCGTCACCCAGCTCGACCTGATCGGACAGGGCGCTTTGCCATCCGAACGTGATGCGCCACGCAGCCC
GTTCGGGTTCGCAATCCCCGCCGTTCCTGCGGACCGGACCATCGATTCGGTGGCCGAGAGGTTCGCGCTGTTTCGCGCCG
GCCTGCGCCATCACGTCCTGGCTGCGGCCGAGATGCACGATCCACTGACGCAGTCCGAGCAGCTCAGCTTTTCGGTGGAG
TTCGACGGGCTCGGAGCACGCTTGAACAGCTTCGTTCGCGTTCTGCTCGGCAATAACCCCTACCACGTACAGCCCGCCCT
GCGCGGTGTCTATTTCAGTTCGCTGCCCGCGGGTGGCGGACATACGGTTCTTGGGCCGAGAACGGATGCGCTGGCGCAGC
GGTTCGAACTCGACCTGCGCGATACGCGTTCTTCGGGCTTCAGCCACGACAGGCAAGACGCCCCCTCTCCGGCCAGGCTC
TTCCACGACGTCGTGCTTCCGGACCGGTTTTTCGCCCGCCGGCTGAGGAGCGCCAGCGCGAGCCAAGGCCGGTGGGCGTG
GATGGCGACCAGCCTCGCCGTGGTCTGCCTTGCCGTCGGTCTGATGACCGCGTCGTTTACCGGTAATCACCAGCTACTAA
CCGAACTCGCAGCGTCCCAGCAGGCTGCGCAGACTTCCGGCGACGGCGTTCCGCTTGCCGTCGAGCTGGAACGACTCGTG
GCCCTTCAGCACCAACTCGAGCGCCTTCGGCGGTACCGGTTGGCGCAACCGCCGTGGCGGCTTCGGTTCGGCCTTTATCA
GGGCGACAAGGTGGAGGCAATGCTGCGCGCGCGCTACTTTGCCGGTGTGCGGCAACTGATGCTGGAACCGGTCGCGCGCG
AGATCGAGCGCGAGCTGGGCGCGACGGCAAGTGCTCCCGTGAGGCGTGCGGGTGCGGGACGCCCTGCCGGGGCGGTCACG
GCCAACGACCGTCCCCCGGCGGGGCTGCCGATCATTCCCCTCGGCGAGGGGGCGGCGGCGCAGCCGCTGCGGGCGGCGGG
AATTCCCCCTGGCGGCCCGGCGTTGTCGGCGCGAGTCCGCCGCTCCGCGGGTGAGTCTGCCGGCATCGAGGGGCAATACA
ACGCGCTCAAGACCTATCTGATGCTCGCCGATCCCTCCCGTATCGAGGCTGAGCATCTGGCTGATCAGTTGCCGCGCTTC
TGGCGCCGCTGGCTTCAGGCCGAACGGGGCCAGACGCCTTTGCCGGATATCTACGCGCTCGCGCAGCGCATGGTGGACTT
CTACATCGAAGAGCGGGCGGCGCCTGACCTCCCCGTGATCGCTGCTGAAGGCGCGCTCGTTGCGCGATCACGCTCCGCCC
TGATAGGCGCAATGCGGCGCCTGCCGCCCGAGGAGCGGATCTATCGCGCGCTGCTTGCTGCCGCGAATGTGAGATTCGAG
CCGGTGTCCGTCACCGGGGTCCTTCGCGATCACGCTAGCGACCTGGTCGGTGGCAGCGTTGTCGTGCCCGGCGCGTTCAC
CCGCAAGGCATACGACGAATACCTGCGCTCGGCATTTGGCCTTTCCGGGCGGGACCTCGACACCGGCACCGACTGGGTTC
TCGACGACCACGCCGGAAATCTAGCGGAATCCAGCAGCGGCGCCGTCGACCCCGGGGCGCTGGAGCGCCGCTACCGCGAG
GACTTCGAGATTGCGTGGCTCGGGTTCCTGCAGGGACTGACGGTGCCCGTCTTCGCGGACCTTGCGGCCGCAACGGACGC
GCTCGGACAGCTCGGCGACCCCGCGAGGTCGCCGTTGCTGCCGCTGCTGCAGCGGGCGGGTCACGAACTGGGCGGGCGGG
GACGCGCGGTGGAGGGGGAGAGGGGGCGCATGGAAACCGTGCTGGCGCGCGCCCGCGGCGTGCTGGACGCCGGGCGGAGT
GAGGCGGTAGCCGACGCAGGTCCGGCATCGGAAGCCAGCCCGCTGCCAGGCGTCGTGGCACTGGCGGGCGGACCGGTGGA
AGCAGGCGACCCGCGCCTCGACGGGGCGCTAAGGGAATACCTCGCCCGGCTGTTGAAGCTGCGAGACCGTCTGGCCGTCG
TCGCTGCCGCGGGGAGCGACGCCGAGCCTGCCATACGGCTCGTCCGCGCAACCTTCGACGGCCGGGAGTCCGAGTTTCCG
GCCAGCCTCTCGTTCGTCGACAACGAGTTGCTGGGGGCGATGGATTCCGCCTCCCGAACGGCTTTGCGACCGGTCCTGAC
GCAGCCCGTGCTGAGAGCGTTCGATGCGCTGATCCCGCTCGTGAGCCGCGATCTCGATCTTGCCTGGCAACGCGAAGTGT
TGCCGCACTGGCGCCGGCTGGCCGAGACTTTTCCCTTCTCCGACAGCGAGCACGATGTGGTGCCGTCCGAACTTGCGGAG
TTCGCGGCACAGGGGGGTGTGCTCGACAGGTTTGTCGAGCGCCGCCTCGAAGGACTTGTAAGCCGACGCGGCCAGGGGCT
GGCAACGCGGAACTGGGGAGATCGCAGCATCGCCCTCAATCCGGCCTTCCTCCCCGCACTGGAGCGATTGCTCGCGTTCG
GTGCGGCGCTCCCGCGTCAGGGGGAACTGGCCCGCTTCGAACTGCAGCCGATGCCGACGCCCGGGCTCACCGAAATCGTG
ATCGAAATCGATGGCCAGAGTCTGCGCTACCGCAACGGGCCCCAGACCTGGGCGAGTTTCAGTTGGCCGGGCACTGCGGT
GCAGGAGGCCGCGGTGCGCGTCGTCGACGTGTCCGGGGCGCCAGGCGCGGTTCTTCACCGTCGCGGGCGCATGGCGCTGA
TCCGGCTGCTGGCCGAGGCCTCCGTCCAGGGCGCCACGCCGGGCCCGCTGCAGTTTGCGTGGAATGGCGCGGGCGCTGCG
GTGCGCTTCAATTTCCGCAGCCTTTCCGGGGCTTCACCGCTCGATCTGCTGGCCTTGCGCAAGCTGACACTGCCCGACCG
GATCAGCCGTTGA