SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001563
Component protein type	TssJ
T6SS ID (Type)	T6SS00160 (Type i4b)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (205 a.a.); Nucleotide sequence (615 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS06590
Coordinate (Strand)	1415603..1416217 (-)
NCBI ID	WP_011765033.1
RefSeq	NC_008702
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.9e-28	58..175

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-204	MNFLDWPALPIFQGCKARRRPSSTRAFIARVPSPAGRILYVCASLFLAGCAVTSTPPRLVLSAETALNRDGSGASLSVVVRTYQLRDRRAFDRLGFDVFGGGRSDTEIFGAELLSSQEFVLTPGARLSDALPLHAEASYVGIVGLFMQPDGELWRGLVDAQATRKLGLQVRLQECYLRIESPAPLKIPGQTEGRTPSCPAGRPS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.8680	pos: 14-15. FQG-CK.	MNFLDWPALPIFQG

Protein sequence: 205 a.a.

						>T6CP001563 NC_008702:c1416217-1415603 [Azoarcus olearius] [TssJ]
MNFLDWPALPIFQGCKARRRPSSTRAFIARVPSPAGRILYVCASLFLAGCAVTSTPPRLVLSAETALNRDGSGASLSVVV
RTYQLRDRRAFDRLGFDVFGGGRSDTEIFGAELLSSQEFVLTPGARLSDALPLHAEASYVGIVGLFMQPDGELWRGLVDA
QATRKLGLQVRLQECYLRIESPAPLKIPGQTEGRTPSCPAGRPS*					

Nucleotide sequence: 615 bp

						>T6CP001563 NC_008702:c1416217-1415603 [Azoarcus olearius] [TssJ]
ATGAATTTTCTGGATTGGCCGGCGTTGCCCATTTTCCAAGGCTGCAAGGCACGACGGCGGCCCTCGTCCACGCGGGCTTT
CATTGCCCGCGTACCGTCGCCGGCAGGTCGCATCCTGTACGTGTGTGCCTCGCTGTTTCTCGCGGGATGCGCAGTCACGA
GCACACCGCCCCGGCTCGTTCTCTCGGCCGAAACCGCGCTGAATCGGGATGGCAGCGGGGCGTCGCTATCGGTCGTGGTA
CGTACCTACCAATTGCGCGACCGGCGCGCCTTCGATCGACTTGGTTTCGACGTATTCGGAGGCGGCAGGTCGGACACGGA
GATATTCGGCGCTGAACTCCTGTCTTCGCAGGAGTTCGTCCTCACGCCGGGCGCACGTCTTTCAGATGCCCTCCCGCTTC
ACGCAGAGGCCAGCTATGTCGGCATCGTCGGGCTGTTCATGCAGCCCGACGGAGAGCTGTGGCGGGGCCTGGTCGATGCC
CAAGCGACCCGTAAGCTAGGGCTGCAGGTACGCCTGCAGGAGTGCTACCTGCGCATCGAAAGTCCCGCCCCATTGAAAAT
CCCCGGCCAGACAGAAGGGCGCACACCCAGTTGCCCCGCCGGCCGCCCCTCCTGA					