SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001561
Component protein type	TssL
T6SS ID (Type)	T6SS00160 (Type i4b)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (233 a.a.); Nucleotide sequence (699 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS06580
Coordinate (Strand)	1413533..1414231 (-)
NCBI ID	WP_011765031.1
RefSeq	NC_008702
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

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Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	9.8e-53	21..220

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Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction	Region	Sequence
Outside	1-199	MAIASTTSAAAVPDRSPGATPLIELLDDGFHLLALLRQRARPRHYDSFVDRVLALLRDFERNALAAGKAPPEIEQARYAFCAALDEVVLSSDFPLRAEWERQPLQLRLFGEHLAGEGFFERLAALRLAPHENIECLEVFHACLLLGFRGKYLLDDSDRIRYLQRTLAQELQRVRGDPQTPPALWKLPEEELTPPRPGLP
Transmembrane helix	200-222	TRFYAGILLVVGLAFFALYQVLL
Inside	223-232	DQRAQALFGV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

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Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8273	-	-

Protein sequence: 233 a.a.

						>T6CP001561 NC_008702:c1414231-1413533 [Azoarcus olearius] [TssL]
MAIASTTSAAAVPDRSPGATPLIELLDDGFHLLALLRQRARPRHYDSFVDRVLALLRDFERNALAAGKAPPEIEQARYAF
CAALDEVVLSSDFPLRAEWERQPLQLRLFGEHLAGEGFFERLAALRLAPHENIECLEVFHACLLLGFRGKYLLDDSDRIR
YLQRTLAQELQRVRGDPQTPPALWKLPEEELTPPRPGLPTRFYAGILLVVGLAFFALYQVLLDQRAQALFGV*					

Nucleotide sequence: 699 bp

						>T6CP001561 NC_008702:c1414231-1413533 [Azoarcus olearius] [TssL]
TTGGCAATTGCCTCCACCACGTCCGCTGCCGCGGTTCCCGACCGCAGCCCTGGCGCAACGCCGCTGATCGAACTGCTGGA
CGACGGATTTCATCTGCTGGCGCTGTTGCGCCAGCGAGCCCGTCCCCGTCACTACGACAGCTTTGTCGACCGGGTGCTGG
CGCTGCTGCGCGACTTCGAGCGAAACGCACTGGCAGCGGGAAAAGCGCCGCCGGAAATCGAGCAGGCGCGCTACGCGTTC
TGCGCGGCGCTCGACGAGGTCGTGCTGTCGAGCGACTTCCCGCTACGGGCCGAGTGGGAAAGGCAACCCCTTCAACTGCG
GCTGTTCGGTGAACACCTGGCTGGGGAGGGCTTCTTCGAACGCCTCGCCGCGTTACGCCTTGCTCCGCACGAGAACATCG
AATGCCTGGAGGTGTTTCATGCCTGCCTGCTACTCGGTTTTCGCGGCAAGTACCTGCTCGACGACAGCGACCGCATCCGC
TACCTGCAGCGCACACTCGCGCAGGAACTGCAACGCGTTCGCGGCGACCCGCAAACGCCCCCCGCGCTGTGGAAGCTGCC
AGAGGAGGAACTGACTCCGCCGCGCCCGGGGTTACCGACCCGGTTTTACGCCGGCATCCTGCTGGTGGTGGGCCTCGCCT
TCTTCGCCCTTTACCAGGTGCTGCTGGACCAGCGCGCGCAAGCGCTGTTCGGCGTCTGA					