Detailed information of component protein


Summary

Component ID T6CP001546
Component protein type TssH
T6SS ID (Type) T6SS00157 experimental (Type i1)
Strain Aeromonas hydrophila subsp. hydrophila ATCC 7966
Replicon chromosome
Sequence Protein sequence (751 a.a.); Nucleotide sequence (2253 bp)
Reference
[1] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190
[2] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[3] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[4] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[5] Khajanchi BK, Sha J, Kozlova EV, et al. N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology (Reading). 2009 Nov;155(Pt 11):3518-3531. doi: 10.1099/mic.0.031575-0. Epub 2009 Sep 3. PMID: 19729404
[6] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[7] Wang N, Wu Y, Pang M, et al. Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015 Oct;46(2):297-304. doi: 10.1016/j.fsi.2015.06.019. Epub 2015 Jun 18. PMID: 26093203
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AHA_RS09370
Coordinate (Strand) 2029151..2031403 (+)
NCBI ID 500025015
RefSeq NC_008570
Uniprot ID A0KJD7_AERHH
KEGG ID aha:AHA_1857
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 1.1e-48 483..643
AAA_lid_9 PF17871.3 2.4e-29 347..450
ClpB_D2-small PF10431.11 2.7e-23 650..730
Clp_N PF02861.22 5.9e-12 13..62
AAA PF00004.31 2.4e-11 208..298
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-750 MLNKDLEKTLNEAFKLARDERHEFMTVEHLLLALLDNSSAHEALSSCGADVELMRKEISAFISQTTPLIPRDDEDRETQPTLGFQRVLQRAVFHVQSSGNTEVSGANVLVAIFSEQESQAAYFLKKAEISRLDVVNFLSHGVRKDSKPDNSSNNAESDDDVSAAQIESFATNLNQLVLEGRIDPLIGRDQELNRTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVKGDVPEVIAGSTIYSLDMGSLLAGTKYRGDFEKRLKVLLKQIERQEGAILFIDEIHTIIGAGAASGGQLDAANLIKPLLSNGLLRCVGSTTYQEYSQIFEKDRALARRFQKIDIVEPSVDDTTRILMGLKSRYEAHHGVRYTVKAIRAAVELSAKYINDRHLPDKAIDVIDEAGAGQRLLPASKRKKVINVQEIEAIVAKIARIPEKSVSSSDKEVLKNLERNLKMVVFGQDKAIEVLTDSIRLSRSGLGNERRPVGCFLFAGPTGVGKTEVTQQLGKALGVEVLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDSVLKHPHSVVLLDEIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNVILVMTTNAGVQETQRKSIGFQQQDMSHDAMAVINKTFSPEFRNRLDHTIWFNHLDMQVIHQVVDKFIVELQVQLDAKGVSLEVSEGVRHWLAEKGYDRAMGARPMGRVIQEHLKKPLANEILFGSLVDGGGVKVELINDSLSFDYQVNVEAVSH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9509 - -


  Protein sequence: 751 a.a.    

>T6CP001546 NC_008570:2029151-2031403 [Aeromonas hydrophila subsp. hydrophila ATCC 7966] [TssH]
MLNKDLEKTLNEAFKLARDERHEFMTVEHLLLALLDNSSAHEALSSCGADVELMRKEISAFISQTTPLIPRDDEDRETQP
TLGFQRVLQRAVFHVQSSGNTEVSGANVLVAIFSEQESQAAYFLKKAEISRLDVVNFLSHGVRKDSKPDNSSNNAESDDD
VSAAQIESFATNLNQLVLEGRIDPLIGRDQELNRTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVKGDVPEVIAGS
TIYSLDMGSLLAGTKYRGDFEKRLKVLLKQIERQEGAILFIDEIHTIIGAGAASGGQLDAANLIKPLLSNGLLRCVGSTT
YQEYSQIFEKDRALARRFQKIDIVEPSVDDTTRILMGLKSRYEAHHGVRYTVKAIRAAVELSAKYINDRHLPDKAIDVID
EAGAGQRLLPASKRKKVINVQEIEAIVAKIARIPEKSVSSSDKEVLKNLERNLKMVVFGQDKAIEVLTDSIRLSRSGLGN
ERRPVGCFLFAGPTGVGKTEVTQQLGKALGVEVLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDSVLKHPHSVVLL
DEIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNVILVMTTNAGVQETQRKSIGFQQQDMSHDAMAVINKTFSPEFRNR
LDHTIWFNHLDMQVIHQVVDKFIVELQVQLDAKGVSLEVSEGVRHWLAEKGYDRAMGARPMGRVIQEHLKKPLANEILFG
SLVDGGGVKVELINDSLSFDYQVNVEAVSH*

  Nucleotide sequence: 2253 bp    

>T6CP001546 NC_008570:2029151-2031403 [Aeromonas hydrophila subsp. hydrophila ATCC 7966] [TssH]
ATGTTGAATAAAGATCTAGAAAAGACGCTGAACGAGGCTTTCAAACTGGCCCGTGACGAGCGCCACGAATTCATGACTGT
CGAGCACCTGTTGTTAGCCCTGCTGGATAACAGCTCAGCCCATGAGGCGCTGAGTTCTTGTGGTGCTGACGTGGAGCTGA
TGCGCAAAGAGATCAGCGCATTCATCAGTCAAACAACCCCGCTTATTCCCCGTGACGATGAAGATCGTGAAACCCAGCCC
ACACTGGGCTTCCAGCGGGTGCTGCAACGCGCCGTGTTCCATGTCCAATCATCGGGCAACACGGAAGTCAGTGGTGCCAA
CGTGCTGGTTGCCATTTTCAGCGAGCAGGAGTCCCAGGCCGCTTACTTCCTGAAAAAGGCCGAGATCAGCCGGCTGGACG
TGGTCAACTTCCTCTCCCATGGCGTGCGCAAAGACAGCAAGCCGGACAACAGCAGCAACAATGCCGAGTCCGACGACGAT
GTGTCTGCCGCCCAGATAGAAAGCTTTGCCACCAATCTCAATCAGCTGGTGCTGGAAGGGCGTATCGATCCGCTCATCGG
GCGGGATCAGGAGCTCAATCGCACCATCCAGGTGCTCTGCCGCCGTCGCAAGAACAACCCCTTACTGGTTGGTGAAGCCG
GGGTAGGCAAGACGGCTATCGCCGAAGGACTGGCTTATCGTATCGTCAAGGGCGATGTGCCCGAGGTGATTGCGGGCAGC
ACCATTTATTCCCTGGACATGGGCTCGCTGCTGGCGGGGACCAAGTATCGCGGTGATTTCGAGAAGCGCCTCAAGGTGCT
GCTCAAGCAGATCGAGCGCCAGGAAGGGGCGATTCTGTTCATCGACGAGATCCACACCATCATAGGGGCAGGGGCGGCCT
CCGGTGGTCAGCTGGACGCGGCCAACCTTATCAAGCCGCTGCTCTCCAATGGTTTGCTGCGCTGTGTCGGCTCCACCACC
TATCAGGAATATTCCCAGATCTTTGAGAAGGACCGTGCTCTGGCGCGTCGCTTCCAGAAGATCGATATCGTCGAGCCCAG
TGTGGATGACACCACCCGCATCCTCATGGGTCTCAAGAGCCGCTATGAGGCTCACCACGGGGTGCGCTACACCGTCAAGG
CCATCCGCGCCGCGGTGGAGCTCTCTGCCAAGTACATCAATGATCGCCATCTGCCTGACAAGGCGATCGACGTGATCGAC
GAAGCCGGTGCCGGACAACGGCTGCTGCCGGCGAGCAAGCGCAAGAAAGTGATCAATGTGCAGGAGATCGAAGCCATTGT
GGCCAAGATTGCCCGCATCCCGGAGAAATCAGTCTCCTCTTCCGACAAGGAAGTGCTGAAGAATCTGGAGCGCAACCTCA
AGATGGTGGTATTTGGTCAGGACAAGGCCATCGAGGTGCTGACCGATTCCATTCGCTTGTCACGCTCGGGCCTTGGTAAT
GAGCGTCGCCCGGTGGGTTGCTTCCTGTTTGCCGGCCCGACCGGGGTGGGCAAGACCGAGGTTACTCAGCAGTTGGGCAA
GGCGCTCGGCGTCGAGGTGCTGCGTTTTGACATGTCGGAGTACATGGAACGCCACACCGTCTCTCGCCTGATCGGGGCTC
CTCCCGGCTATGTGGGCTTTGAGCAGGGTGGACTCCTGACCGACTCGGTACTCAAGCATCCGCACTCAGTGGTGCTGCTG
GACGAGATCGAAAAAGCGCACCCGGACGTGTTCAACCTGCTGCTGCAGGTGATGGACAACGGCACCCTGACCGACAACAA
CGGGCGCAAGGCGGATTTTCGCAATGTGATCCTGGTGATGACCACCAACGCCGGCGTGCAGGAGACCCAGCGCAAATCCA
TCGGCTTCCAGCAACAGGATATGAGCCATGATGCCATGGCGGTGATCAACAAGACGTTCAGCCCGGAGTTTCGTAACCGG
CTCGATCACACCATCTGGTTCAACCATCTGGATATGCAGGTGATACACCAGGTGGTCGACAAGTTCATCGTCGAGTTGCA
GGTACAACTGGATGCCAAGGGCGTGTCTCTTGAAGTGTCCGAGGGTGTCCGCCACTGGTTGGCCGAGAAGGGATATGACA
GGGCCATGGGAGCGAGACCCATGGGGCGGGTCATCCAGGAACACCTCAAGAAACCCCTTGCCAATGAGATCCTGTTCGGA
TCCTTGGTCGATGGGGGGGGAGTGAAGGTTGAGTTGATAAATGACAGCCTCAGCTTTGACTATCAGGTCAATGTTGAAGC
TGTTTCCCACTAA