Detailed information of component protein


Summary

Component ID T6CP001544
Component protein type TssM
T6SS ID (Type) T6SS00157 experimental (Type i1)
Strain Aeromonas hydrophila subsp. hydrophila ATCC 7966
Replicon chromosome
Sequence Protein sequence (1162 a.a.); Nucleotide sequence (3486 bp)
Reference
[1] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190
[2] Suarez G, Sierra JC, Kirtley ML, et al. Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology (Reading). 2010 Dec;156(Pt 12):3678-3688. doi: 10.1099/mic.0.041277-0. Epub 2010 Aug 26. PMID: 20798163
[3] Suarez G, Sierra JC, Sha J, et al. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 2008 Apr;44(4):344-61. Epub 2007 Oct 24. PMID: 18037263
[4] Suarez G, Sierra JC, Erova TE, et al. A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 2010 Jan;192(1):155-68. doi: 10.1128/JB.01260-09. PMID: 19880608
[5] Khajanchi BK, Sha J, Kozlova EV, et al. N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology (Reading). 2009 Nov;155(Pt 11):3518-3531. doi: 10.1099/mic.0.031575-0. Epub 2009 Sep 3. PMID: 19729404
[6] Sha J, Rosenzweig JA, Kozlova EV, et al. Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology (Reading). 2013 Jun;159(Pt 6):1120-1135. doi: 10.1099/mic.0.063495-0. Epub 2013 Mar 21. PMID: 23519162
[7] Wang N, Wu Y, Pang M, et al. Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015 Oct;46(2):297-304. doi: 10.1016/j.fsi.2015.06.019. Epub 2015 Jun 18. PMID: 26093203
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AHA_RS09295
Coordinate (Strand) 2014151..2017636 (+)
NCBI ID 500025007
RefSeq NC_008570
Uniprot ID A0KJC8_AERHH
KEGG ID aha:AHA_1845
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 5.4e-74 502..815
IcmF-related_N PF14331.8 8.3e-72 201..450
IcmF_C PF06744.14 3.8e-22 1034..1141
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MFKTIFTFLRQQLPKLKPS
Transmembrane helix 20-39 WPLLGAVLWVLALILVWWLG
Outside 40-53 PRLEVRGAKPFEPL
Transmembrane helix 54-76 WGRVVFTLLWLWLLLGVVSWRVW
Inside 77-445 RKMQQLKAERQHEVVLEQDPVKGLIDRQALFLDRWLQALNTHLGKGALYAMPWYLVLGLPGSGKSSLIHRANPANKLNPRLDTELRDVAQDQLVDCWLGEQAVMLDPAGVLLSQSEAELDPQARKHERLWLHLLGWLNEHRRRQPLNGLVLTVDLAWLSHASVAERKAYAQLMRSRLQEVSATMNTRLPLYVTFTKLDLLRGFDVIYQQLDKEARDAVLGVTFKPGADWQQDLALFWDQWVDNLNQNLPELMLSRLDAAQRNALFSFVRQLAGLKDYVTSLLAETLAIEESKPLLVRGVYVSSVYQQGVPFDAFAQAASRRYNLPEPIHSALRGESNTYFVRQLFSSIIFPEAHLAGENRLHTLYRRRR
Transmembrane helix 446-468 MAIGLSCLSLFSAALIGGWHYFY
Outside 469-1161 RVNEEAGRNVLTKAQAFMETNEVADAHAFGVSQLPRLNLIREATLSFGNYRERMPLVADLGLYQGDEIGPYVEGSYLQLLSLRFLPAQMQGLLADLNQAPAGSEEKLAILRVMRMLDDASGRNKELVAQYMASRWQKAFPGQGAVQEQLMGHLDYALDHTNWYGARAERDQAAITAFVPFKEPVYGAQRELGKLPMYQRVYQNLVVKAGDVLPPDLNVRDEVGPTFDTVFALRSDNAGQVPRLLTWPGFNDFFLKQDKALIDLTAMDAWVLGQRKLSQLSEADRKEITRQVNDRYVTDYVNQWQKLLTNLDVQTLESPEQALDVLAAITGNDQPFQRVLASLDDNTRIRKISDVEGDPAQAISARIGRPFMATNGVLAGRGEQGPLIQEVNQKLVELQHYLELIVNATEPGQSALKAVQLRMTNKYADPVFALQQYARSLPAPLDRWVGQLSEQSSRLVIDLAMSSLNQEWQDKVLTPFNSQLAGRYPFDPSSNKDVPLSEMERFFAPNGTLDSFYQVNLKPMVESGLMEGEFSSPIQAELVKQLDRAARIRQIFFSQQGNLEVQFALEPIELTANKRRSVLNLDGQLLEYAHGRRTKIPLVWPNTMRDGAESKITLVPAARERSPRSEGFVGPWAMFRLMDKGELTQVNDATFDVRFPVDQGAMTYRVYTDSAQNPFTGGLFSQFRLPESLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9940 - -


  Protein sequence: 1162 a.a.    

>T6CP001544 NC_008570:2014151-2017636 [Aeromonas hydrophila subsp. hydrophila ATCC 7966] [TssM]
MFKTIFTFLRQQLPKLKPSWPLLGAVLWVLALILVWWLGPRLEVRGAKPFEPLWGRVVFTLLWLWLLLGVVSWRVWRKMQ
QLKAERQHEVVLEQDPVKGLIDRQALFLDRWLQALNTHLGKGALYAMPWYLVLGLPGSGKSSLIHRANPANKLNPRLDTE
LRDVAQDQLVDCWLGEQAVMLDPAGVLLSQSEAELDPQARKHERLWLHLLGWLNEHRRRQPLNGLVLTVDLAWLSHASVA
ERKAYAQLMRSRLQEVSATMNTRLPLYVTFTKLDLLRGFDVIYQQLDKEARDAVLGVTFKPGADWQQDLALFWDQWVDNL
NQNLPELMLSRLDAAQRNALFSFVRQLAGLKDYVTSLLAETLAIEESKPLLVRGVYVSSVYQQGVPFDAFAQAASRRYNL
PEPIHSALRGESNTYFVRQLFSSIIFPEAHLAGENRLHTLYRRRRMAIGLSCLSLFSAALIGGWHYFYRVNEEAGRNVLT
KAQAFMETNEVADAHAFGVSQLPRLNLIREATLSFGNYRERMPLVADLGLYQGDEIGPYVEGSYLQLLSLRFLPAQMQGL
LADLNQAPAGSEEKLAILRVMRMLDDASGRNKELVAQYMASRWQKAFPGQGAVQEQLMGHLDYALDHTNWYGARAERDQA
AITAFVPFKEPVYGAQRELGKLPMYQRVYQNLVVKAGDVLPPDLNVRDEVGPTFDTVFALRSDNAGQVPRLLTWPGFNDF
FLKQDKALIDLTAMDAWVLGQRKLSQLSEADRKEITRQVNDRYVTDYVNQWQKLLTNLDVQTLESPEQALDVLAAITGND
QPFQRVLASLDDNTRIRKISDVEGDPAQAISARIGRPFMATNGVLAGRGEQGPLIQEVNQKLVELQHYLELIVNATEPGQ
SALKAVQLRMTNKYADPVFALQQYARSLPAPLDRWVGQLSEQSSRLVIDLAMSSLNQEWQDKVLTPFNSQLAGRYPFDPS
SNKDVPLSEMERFFAPNGTLDSFYQVNLKPMVESGLMEGEFSSPIQAELVKQLDRAARIRQIFFSQQGNLEVQFALEPIE
LTANKRRSVLNLDGQLLEYAHGRRTKIPLVWPNTMRDGAESKITLVPAARERSPRSEGFVGPWAMFRLMDKGELTQVNDA
TFDVRFPVDQGAMTYRVYTDSAQNPFTGGLFSQFRLPESLY*

  Nucleotide sequence: 3486 bp    

>T6CP001544 NC_008570:2014151-2017636 [Aeromonas hydrophila subsp. hydrophila ATCC 7966] [TssM]
ATGTTCAAAACCATTTTTACTTTTCTGCGGCAACAGCTGCCGAAATTGAAACCCTCCTGGCCCCTGCTCGGTGCTGTGCT
CTGGGTGCTGGCTCTCATCCTGGTGTGGTGGCTGGGCCCTCGTCTCGAGGTGCGCGGCGCCAAACCGTTCGAGCCGTTGT
GGGGGCGGGTGGTGTTCACCCTGCTCTGGCTCTGGTTGCTGCTGGGAGTGGTCTCCTGGCGGGTGTGGCGCAAGATGCAA
CAGCTCAAGGCCGAACGCCAGCACGAGGTGGTGCTGGAGCAGGACCCGGTCAAGGGGCTCATCGACAGGCAGGCGCTGTT
CCTCGATCGCTGGCTGCAGGCGTTGAATACCCATCTGGGCAAGGGGGCGCTCTACGCCATGCCCTGGTATCTGGTGCTGG
GGCTGCCCGGCAGCGGCAAGAGCAGCCTCATTCACCGCGCCAACCCGGCCAACAAGCTCAACCCGAGACTCGATACCGAA
CTGCGCGACGTGGCGCAGGATCAGCTGGTGGATTGCTGGCTGGGGGAACAGGCGGTGATGCTGGATCCCGCCGGGGTGCT
GCTCTCCCAGAGCGAAGCCGAACTGGATCCGCAGGCCCGCAAGCATGAGCGGCTCTGGCTGCACCTGCTTGGCTGGCTCA
ATGAACACCGCCGCCGCCAGCCCCTCAACGGCCTGGTGCTGACCGTGGATCTGGCCTGGCTCTCCCACGCCAGCGTGGCC
GAGCGCAAGGCCTATGCCCAGCTGATGCGCTCGCGCCTGCAGGAGGTGTCGGCCACCATGAATACCCGGTTGCCGCTCTA
TGTCACCTTCACCAAGCTCGATCTGCTGCGCGGCTTTGACGTTATCTACCAGCAGCTCGACAAGGAGGCCCGGGATGCCG
TGCTGGGGGTGACCTTCAAGCCAGGCGCCGACTGGCAGCAGGATCTGGCCCTGTTCTGGGATCAGTGGGTGGACAACCTC
AACCAGAATCTGCCCGAACTGATGCTGAGCCGGCTCGATGCCGCCCAGCGCAACGCGCTGTTCTCGTTCGTGCGCCAGCT
GGCGGGCCTCAAGGATTACGTGACCAGCCTGCTGGCCGAGACCCTGGCCATCGAGGAGAGCAAGCCGCTGCTGGTGCGCG
GGGTTTATGTCAGCTCCGTCTATCAGCAAGGGGTGCCGTTCGATGCGTTTGCCCAGGCGGCCTCCCGCCGCTACAACTTG
CCAGAGCCCATCCACTCGGCCCTGCGTGGGGAGTCCAACACCTACTTCGTGCGCCAGCTGTTCTCCTCCATCATCTTCCC
GGAGGCCCATCTGGCCGGCGAAAACCGGCTGCACACCCTCTATCGCCGGCGCCGCATGGCCATCGGCCTCAGCTGCCTGT
CGCTCTTCTCGGCCGCCCTCATCGGTGGCTGGCACTACTTCTACCGGGTCAACGAGGAGGCGGGCCGCAACGTATTGACC
AAGGCTCAGGCCTTCATGGAGACCAACGAGGTGGCCGACGCCCACGCCTTCGGCGTGAGCCAGCTGCCGCGCCTCAATCT
TATTCGCGAGGCGACCCTCTCCTTTGGCAACTACCGCGAGCGGATGCCGCTGGTGGCGGATCTCGGCCTCTACCAAGGGG
ACGAGATTGGTCCTTATGTGGAGGGCTCCTACCTGCAACTGCTGAGCCTGCGTTTCCTGCCGGCCCAGATGCAGGGATTG
CTGGCGGATCTCAACCAGGCGCCTGCCGGCAGCGAGGAGAAGCTCGCCATCCTGCGGGTGATGCGGATGCTGGACGATGC
GTCCGGTCGCAACAAGGAGCTGGTGGCGCAGTACATGGCGAGCCGTTGGCAGAAGGCCTTCCCGGGGCAGGGGGCGGTAC
AGGAGCAGCTGATGGGGCACCTGGACTATGCCCTCGACCACACCAACTGGTATGGCGCCAGGGCCGAGCGGGATCAGGCC
GCCATCACCGCCTTCGTGCCGTTCAAGGAGCCGGTCTACGGTGCCCAGCGCGAGCTCGGCAAGCTGCCCATGTATCAGCG
GGTCTACCAGAACCTGGTGGTGAAGGCGGGCGATGTATTGCCGCCGGATCTCAACGTCCGTGACGAGGTGGGCCCCACCT
TTGACACCGTGTTTGCCCTGCGCAGCGACAACGCCGGCCAGGTGCCGCGGCTGCTCACCTGGCCCGGTTTCAACGACTTC
TTCCTGAAACAAGACAAGGCGCTCATCGATCTCACCGCGATGGATGCCTGGGTGCTGGGCCAGCGCAAGCTGAGCCAGTT
GAGCGAGGCAGATCGCAAGGAGATCACCCGTCAGGTCAACGACCGTTACGTCACCGATTACGTCAACCAGTGGCAAAAAC
TGCTGACCAATCTGGACGTGCAGACCCTGGAGAGCCCGGAGCAGGCGCTGGACGTGCTGGCCGCCATTACTGGCAACGAT
CAGCCGTTCCAACGGGTACTGGCCTCGCTGGATGACAACACCCGCATTCGCAAGATCTCCGATGTGGAAGGGGACCCGGC
CCAGGCCATCAGCGCCCGCATCGGTCGTCCCTTTATGGCCACTAATGGAGTATTGGCTGGACGTGGCGAGCAGGGACCGC
TCATTCAGGAGGTCAACCAGAAGCTGGTGGAGCTGCAGCACTACCTGGAGCTCATCGTCAACGCCACCGAGCCGGGCCAG
TCCGCCTTGAAAGCGGTGCAGCTGCGCATGACCAACAAGTACGCCGATCCGGTGTTCGCCCTGCAGCAATATGCCCGCAG
TCTGCCGGCCCCGCTCGATCGCTGGGTGGGTCAGCTCTCCGAGCAGAGCTCGCGGCTGGTGATCGACCTGGCCATGTCCT
CCCTCAATCAGGAGTGGCAGGACAAGGTGCTGACCCCCTTCAACAGCCAGCTGGCCGGTCGTTATCCGTTTGATCCGAGC
TCGAACAAGGATGTGCCCCTCTCCGAGATGGAGCGCTTCTTTGCGCCTAACGGGACGCTGGACAGCTTCTATCAGGTCAA
CCTCAAACCCATGGTGGAGAGCGGCCTGATGGAAGGGGAGTTCAGCTCGCCCATCCAGGCCGAGCTGGTCAAGCAGCTGG
ACCGTGCGGCCCGCATCCGTCAGATCTTCTTCAGCCAGCAGGGCAACCTGGAGGTGCAGTTCGCCCTCGAGCCCATCGAG
CTCACCGCCAACAAGCGGCGCAGTGTGCTGAACCTGGACGGCCAGCTACTGGAATATGCCCATGGCCGTCGTACCAAGAT
CCCGCTGGTGTGGCCCAATACCATGCGCGACGGGGCGGAGAGCAAGATCACTCTGGTGCCGGCCGCCCGCGAGCGCTCAC
CACGCAGCGAAGGCTTCGTCGGTCCCTGGGCAATGTTCCGTCTGATGGACAAGGGGGAGCTGACCCAGGTCAATGACGCC
ACCTTCGATGTGCGCTTCCCGGTGGATCAGGGGGCCATGACCTATCGCGTCTACACCGACAGCGCGCAAAACCCCTTCAC
CGGCGGCCTGTTCAGCCAGTTCAGACTGCCTGAATCCCTCTATTGA