SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001523
Component protein type	TssB
T6SS ID (Type)	T6SS00155 (Type i1)
Strain	Escherichia coli APEC O1
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	APECO1_RS01215
Coordinate (Strand)	262509..263012 (-)
NCBI ID	WP_000041480.1
RefSeq	NC_008563
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.1e-44	10..161

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 168 a.a.

						>T6CP001523 NC_008563:c263012-262509 [Escherichia coli APEC O1] [TssB]
MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNLKGKPDNTPLDERTAIAINRYNLNAVISEADIEREFV
VPAELSDAPNEQMYVNLKVKSMDDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
LGIAVQK*					

Nucleotide sequence: 504 bp

						>T6CP001523 NC_008563:c263012-262509 [Escherichia coli APEC O1] [TssB]
ATGAGCAAAATGAACAACAATGGCGGCAGTGTCGCGCCGAAAGAAAGAATCAGCGTTCGGTATACACCAAAAGTTGATGG
GGTGGCTGCTGATATCGAATTGCCGCTGAATTTATTGATCACCGGCAATCTGAAAGGGAAACCAGATAACACGCCGTTGG
ATGAGCGAACTGCCATTGCTATCAACCGCTATAACCTCAACGCAGTCATTTCAGAAGCTGACATTGAACGGGAGTTTGTT
GTTCCGGCTGAACTCAGTGACGCTCCCAATGAGCAAATGTACGTCAACCTGAAAGTCAAATCTATGGATGACCTTTCTCC
TGACCATATTGCCAGCCAGGTACCAGAAATAAAACGTCTGCTCGAATTGCGTGAAGCCCTAGTCGCACTTAAGTCTCCAC
TAGGCAATATTCCAGCCTTCCGCGCACAACTTCAGGCGCTACTGGAAAACGAAGACACCCGCGAGCAGCTGATCCAGGAA
CTGGGTATCGCCGTTCAGAAATAA					