SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001519
Component protein type	TssJ
T6SS ID (Type)	T6SS00155 (Type i1)
Strain	Escherichia coli APEC O1
Replicon	chromosome
Sequence	Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	APECO1_RS01185
Coordinate (Strand)	255894..256418 (-)
NCBI ID	WP_001013423.1
RefSeq	NC_008563
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.7e-23	45..164

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 175 a.a.

						>T6CP001519 NC_008563:c256418-255894 [Escherichia coli APEC O1] [TssJ]
MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKF
HAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLV
HIRASAIEMKKEEN*					

Nucleotide sequence: 525 bp

						>T6CP001519 NC_008563:c256418-255894 [Escherichia coli APEC O1] [TssJ]
ATGAACGGCAAAGCGTTTCTGGCCTGCGTTCTGATGAGCGTCGTATTAACTGGCTGTGAAACAGCGAAAAAAATCAGCCA
GGTGATCCGCAATCCGGATATTCAGGTCGGAAAGCTGATGGATCAGTCAACCGAGCTGACCGTCACGCTGCTGACCGAGC
CGGACAGCAACCTGACGGCGGATGGCGAAGCCGCGCCGGTGGATGTCCAGTTGGTTTATCTGAGCGACGACTCAAAATTC
CATGCCGCCGACTACGACCAGGTTGCCACCACCGCGCTGCCCGACGTGCTGGGGAAAAACTATATCGATCACCAGGACTT
CAACCTGTTGCCGGATACCGTAAAAACACTGCCGCCGATCAAGTTGGATGAGAAAACCGGTTATATCGGTGTCATTGCCT
ATTTTTCAGACGACCAGGCCACAGAATGGAAACAAATTGAGTCGGTAGAAAGTATCGGCCACCACTATCGCCTGCTGGTG
CATATCCGCGCCAGTGCGATTGAGATGAAAAAAGAGGAAAACTGA					