Detailed information of component protein


Summary

Component ID T6CP001229
Component protein type TssI
T6SS ID (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome I
Sequence Protein sequence (1064 a.a.); Nucleotide sequence (3192 bp)
Reference
[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[2] Perault AI, Chandler CE, Rasko DA, et al. Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI Secretion System-Mediated Predation by the Burkholderia cepacia Complex. Cell Host Microbe. 2020 Oct 7;28(4):534-547.e3. doi: 10.1016/j.chom.2020.06.019. Epub 2020 Aug 4. PMID: 32755549
[3] Loeven NA, Perault AI, Cotter PA, et al. The Burkholderia cenocepacia Type VI Secretion System Effector TecA Is a Virulence Factor in Mouse Models of Lung Infection. mBio. 2021 Oct 26;12(5):e0209821. doi: 10.1128/mBio.02098-21. Epub 2021 Sep 28. PMID: 34579569
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BCEN_RS13185
Coordinate (Strand) 2916636..2919827 (-)
NCBI ID WP_011546587.1
RefSeq NC_008060
Uniprot ID A0A0H2XSS4_BURCA
KEGG ID bcn:Bcen_2648
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 6.6e-85 41..353
DUF2345 PF10106.11 2e-55 670..819
T6SS_Vgr PF13296.8 3.7e-33 525..629
Phage_base_V PF04717.14 5.5e-11 438..505
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1063 MNMQNTIAALRGGLLQQDRLLKLDTPLGSNVLTVQRAVGRSRIGRAYEFTLDVLSTDSDLELKKLIAQPVTLWIQQGDRSYRPMNGYVHTARRLGADGGLTTYQLTFADFTHFLKFRRDQRIWNDTTVDQIISDVLNQHPQAKGHFRFALSKPLPNRSYTRQHDTDWHFIHRLMEDEGLYCAWQQADDGKSHTLVVTDNLQAFAPLSPQTVRFYRGGAASEADAFTQWSGTRTLQSVTRSTRTFDYKNPAQPSNPKGTSLPTMAGQGELPDQLEVYEYTGAYTYLDQTRGDHLTKIRMEQWESEAKRFHGAGGVRAIDAGRRFTLADHPEHDRDPADQREFAAIGVEWWIENNLPVSNGSTDFPYSLREALTQAQEGYGADPAFRVPHDDGSVGFYLVEVEAQRVNVPYRSPFEHKKPEMHLETAIVVGPQGEEVYTDELNRTRVQFVWDRLNPGNENASCWVRVVQSDTGGGYGGVHVPRIGEEVLIDYVGGDCDRPLAVGRVYNGANQPQWHSDGILSGYRSKEYSGGGYNQLVMDDATGQNRVQLMSSSANSLLHLGYIIDQNGNARGSYLGSGFDLRSDAYGAVRASQGLYVTTHPKAANSQPLDVKEAQQQLVAGESLVEAMSGVSEQHQAESLKDAQDTMRAFTDATQDSQSGSAAGGRTAGGGTGNANAFKEPVMLFGSPSGIGMSTQQSVHMVANDHVNVASGQSVHVAAGKSLIGSIGQKLSLFVQNAGMKLFAGKGKVEIQAQSDNIEVTAQKAVRVVSATDRIEIAADQGILLTSGGGYIRIKDGDIEIHTPGAVDVKGASHTFAGPASMGYPLPSPRPDQPGQLELFHKYVNGEAVKGGLFTVKDVNGAVLKKGALDNSGYTVVSGLPPGAVRVEFGKDPRESDQPANYFKQAKWPAEPVQPSPEAAQAAASGQLASQLQGMAPAATTAAMGLVSGGGAGAALGGLASAALPAAATALGGAGVASALQTASSLSGAAKQVAGMVQAARQGGLAALAAPAANAASGALQGALPGALPGVAGIAGKGATTVASAAGATGMKLPTSGFGGPLKS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9893 - -


  Protein sequence: 1064 a.a.    

>T6CP001229 NC_008060:c2919827-2916636 [Burkholderia cenocepacia AU 1054] [TssI]
MNMQNTIAALRGGLLQQDRLLKLDTPLGSNVLTVQRAVGRSRIGRAYEFTLDVLSTDSDLELKKLIAQPVTLWIQQGDRS
YRPMNGYVHTARRLGADGGLTTYQLTFADFTHFLKFRRDQRIWNDTTVDQIISDVLNQHPQAKGHFRFALSKPLPNRSYT
RQHDTDWHFIHRLMEDEGLYCAWQQADDGKSHTLVVTDNLQAFAPLSPQTVRFYRGGAASEADAFTQWSGTRTLQSVTRS
TRTFDYKNPAQPSNPKGTSLPTMAGQGELPDQLEVYEYTGAYTYLDQTRGDHLTKIRMEQWESEAKRFHGAGGVRAIDAG
RRFTLADHPEHDRDPADQREFAAIGVEWWIENNLPVSNGSTDFPYSLREALTQAQEGYGADPAFRVPHDDGSVGFYLVEV
EAQRVNVPYRSPFEHKKPEMHLETAIVVGPQGEEVYTDELNRTRVQFVWDRLNPGNENASCWVRVVQSDTGGGYGGVHVP
RIGEEVLIDYVGGDCDRPLAVGRVYNGANQPQWHSDGILSGYRSKEYSGGGYNQLVMDDATGQNRVQLMSSSANSLLHLG
YIIDQNGNARGSYLGSGFDLRSDAYGAVRASQGLYVTTHPKAANSQPLDVKEAQQQLVAGESLVEAMSGVSEQHQAESLK
DAQDTMRAFTDATQDSQSGSAAGGRTAGGGTGNANAFKEPVMLFGSPSGIGMSTQQSVHMVANDHVNVASGQSVHVAAGK
SLIGSIGQKLSLFVQNAGMKLFAGKGKVEIQAQSDNIEVTAQKAVRVVSATDRIEIAADQGILLTSGGGYIRIKDGDIEI
HTPGAVDVKGASHTFAGPASMGYPLPSPRPDQPGQLELFHKYVNGEAVKGGLFTVKDVNGAVLKKGALDNSGYTVVSGLP
PGAVRVEFGKDPRESDQPANYFKQAKWPAEPVQPSPEAAQAAASGQLASQLQGMAPAATTAAMGLVSGGGAGAALGGLAS
AALPAAATALGGAGVASALQTASSLSGAAKQVAGMVQAARQGGLAALAAPAANAASGALQGALPGALPGVAGIAGKGATT
VASAAGATGMKLPTSGFGGPLKS*

  Nucleotide sequence: 3192 bp    

>T6CP001229 NC_008060:c2919827-2916636 [Burkholderia cenocepacia AU 1054] [TssI]
ATGAACATGCAGAACACAATCGCCGCATTGCGCGGCGGATTGCTTCAGCAGGACCGGTTGTTGAAGCTCGATACGCCATT
GGGGAGCAACGTCCTGACCGTGCAGCGGGCAGTTGGCCGCTCACGTATCGGGCGCGCGTACGAATTCACGCTGGACGTAC
TGTCGACCGATAGCGATCTGGAGCTTAAAAAGCTGATCGCGCAGCCGGTCACGTTGTGGATTCAGCAAGGCGATCGCAGC
TATCGGCCGATGAACGGCTACGTGCACACCGCGCGCCGTCTGGGCGCCGACGGCGGGCTCACGACCTATCAGCTCACGTT
CGCCGATTTCACCCATTTCCTGAAGTTCCGTCGCGACCAGCGGATCTGGAACGATACGACCGTCGACCAGATCATCAGCG
ACGTGCTGAACCAGCATCCGCAGGCGAAAGGCCATTTCCGCTTTGCGCTGTCGAAGCCGTTGCCGAACCGCTCGTACACG
CGACAGCACGACACCGACTGGCATTTCATCCATCGACTGATGGAGGACGAAGGGCTCTACTGCGCGTGGCAGCAGGCCGA
CGACGGCAAGTCGCACACGCTCGTGGTGACCGACAACCTGCAGGCGTTCGCGCCGCTATCGCCGCAGACCGTGCGCTTCT
ATCGTGGCGGGGCGGCGAGCGAAGCCGATGCGTTCACGCAATGGTCGGGCACGCGCACGCTGCAGAGCGTCACGCGTTCG
ACGCGCACGTTCGACTACAAGAATCCCGCGCAGCCGTCGAATCCGAAAGGCACGTCGCTGCCGACGATGGCCGGTCAGGG
CGAATTGCCCGATCAGCTCGAAGTCTACGAATACACCGGTGCGTACACGTATCTGGACCAGACGCGCGGCGACCATCTGA
CGAAGATCCGGATGGAGCAGTGGGAGTCGGAGGCGAAGCGCTTCCACGGCGCGGGCGGCGTGCGTGCGATCGATGCCGGC
CGGCGCTTCACGCTGGCGGATCATCCCGAACACGATCGCGATCCGGCCGACCAGCGCGAATTCGCGGCGATCGGCGTCGA
ATGGTGGATCGAGAACAACCTCCCCGTTTCGAACGGCAGCACGGATTTTCCGTACAGCCTGCGTGAAGCGTTGACGCAAG
CGCAGGAGGGCTACGGTGCCGACCCCGCGTTTCGCGTGCCGCACGACGACGGCTCGGTCGGCTTCTATCTCGTCGAGGTC
GAAGCGCAGCGCGTGAACGTGCCGTATCGCAGCCCGTTCGAGCATAAAAAGCCGGAGATGCACCTCGAGACGGCGATCGT
CGTCGGGCCGCAGGGCGAGGAGGTTTATACCGACGAGCTGAACCGGACTCGCGTGCAGTTCGTGTGGGATCGCCTGAACC
CGGGCAACGAGAACGCATCGTGCTGGGTGCGCGTCGTGCAGTCGGATACGGGCGGCGGCTACGGCGGTGTGCACGTGCCG
CGCATCGGCGAGGAAGTGCTGATCGACTACGTCGGCGGCGACTGCGACCGGCCGCTGGCCGTGGGGCGCGTGTACAACGG
CGCGAACCAGCCGCAGTGGCATAGCGACGGGATTCTGTCCGGCTATCGATCGAAGGAGTATTCGGGCGGCGGCTACAACC
AGCTCGTGATGGACGACGCGACCGGGCAGAACCGCGTGCAACTGATGAGCAGCAGCGCGAACAGCCTGCTGCATCTCGGC
TACATCATCGACCAGAACGGCAATGCGCGCGGGTCGTATCTGGGCAGCGGTTTCGATCTGCGCTCGGATGCGTATGGCGC
GGTGCGGGCGAGCCAGGGCCTGTACGTGACGACGCACCCGAAGGCGGCGAACAGCCAGCCGCTCGACGTGAAGGAGGCGC
AGCAGCAGCTCGTGGCGGGCGAGAGCCTGGTCGAGGCGATGTCGGGCGTGAGCGAGCAGCATCAGGCGGAGAGCCTGAAG
GACGCGCAGGACACGATGCGTGCATTCACGGACGCGACGCAGGACAGCCAGTCGGGCAGCGCGGCGGGCGGGCGCACGGC
GGGCGGCGGCACCGGTAACGCGAACGCGTTCAAGGAACCGGTGATGCTGTTCGGCAGCCCGTCGGGAATCGGGATGTCGA
CGCAGCAGTCGGTGCATATGGTCGCGAACGATCACGTGAACGTGGCAAGCGGGCAGAGCGTGCACGTGGCCGCGGGCAAG
TCGCTGATCGGCAGCATCGGGCAGAAGCTGAGCCTGTTCGTGCAGAACGCGGGGATGAAGCTGTTCGCGGGCAAGGGCAA
GGTCGAGATCCAGGCGCAGTCGGACAACATCGAGGTGACCGCGCAGAAGGCGGTGAGGGTGGTGTCGGCGACGGACCGGA
TCGAGATCGCGGCCGATCAGGGGATTTTGCTGACGAGCGGCGGCGGATATATCCGCATCAAGGACGGCGACATCGAGATT
CATACGCCGGGCGCGGTCGATGTGAAGGGGGCGTCGCATACGTTCGCGGGGCCGGCGAGCATGGGGTATCCGCTGCCGAG
CCCGCGGCCGGATCAGCCCGGGCAGCTCGAACTGTTTCACAAGTACGTGAACGGCGAGGCGGTGAAGGGCGGGCTGTTCA
CGGTGAAGGACGTGAACGGCGCCGTGCTGAAGAAAGGCGCACTCGACAACAGCGGCTATACGGTCGTGAGCGGCTTGCCG
CCGGGGGCGGTGCGCGTCGAATTCGGGAAAGACCCACGCGAGTCGGATCAGCCGGCGAACTATTTCAAGCAGGCGAAGTG
GCCGGCGGAGCCCGTGCAACCGTCGCCCGAGGCCGCGCAGGCCGCGGCGAGCGGACAGCTCGCGAGTCAGCTGCAAGGGA
TGGCGCCGGCCGCGACGACCGCGGCGATGGGGCTGGTGAGCGGCGGCGGCGCGGGTGCGGCCCTCGGCGGGCTCGCGAGC
GCCGCATTGCCGGCTGCTGCGACCGCGTTGGGTGGCGCCGGCGTGGCGTCCGCGTTGCAGACCGCCTCGAGCCTGAGCGG
TGCGGCGAAGCAGGTGGCCGGGATGGTTCAGGCCGCCCGTCAGGGCGGGCTTGCCGCGCTTGCCGCGCCGGCTGCGAATG
CGGCGTCCGGGGCGTTGCAGGGCGCGTTGCCCGGTGCATTGCCGGGCGTGGCCGGTATCGCGGGCAAGGGCGCCACGACC
GTGGCGTCGGCCGCGGGCGCGACGGGCATGAAACTGCCGACAAGCGGGTTTGGCGGACCGTTGAAATCCTGA