Detailed information of component protein


Summary

Component ID T6CP001218
Component protein type TssA
T6SS ID (Type) T6SS00126 experimental (Type i4b)
Strain Burkholderia cenocepacia AU 1054
Replicon chromosome I
Sequence Protein sequence (374 a.a.); Nucleotide sequence (1122 bp)
Reference
[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[2] Perault AI, Chandler CE, Rasko DA, et al. Host Adaptation Predisposes Pseudomonas aeruginosa to Type VI Secretion System-Mediated Predation by the Burkholderia cepacia Complex. Cell Host Microbe. 2020 Oct 7;28(4):534-547.e3. doi: 10.1016/j.chom.2020.06.019. Epub 2020 Aug 4. PMID: 32755549
[3] Loeven NA, Perault AI, Cotter PA, et al. The Burkholderia cenocepacia Type VI Secretion System Effector TecA Is a Virulence Factor in Mouse Models of Lung Infection. mBio. 2021 Oct 26;12(5):e0209821. doi: 10.1128/mBio.02098-21. Epub 2021 Sep 28. PMID: 34579569
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BCEN_RS13115
Coordinate (Strand) 2896514..2897635 (-)
NCBI ID WP_011546577.1
RefSeq NC_008060
Uniprot ID A0A0H2XTR5_BURCA
KEGG ID bcn:Bcen_2631
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 2.3e-36 9..131
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-373 MPINLPELLTPISDASPSGDDLLFSNEFDAIQDARRYDDPTLDQGEWVTEIKEADWGFVVDHAGELLRTRTKDLRLAVWLTEALALEDGITGLTEGYALLEGLCRDFWDTFHPLPEDDDIEHRLGNVAWLSGRTAELLRAVPLTDGASNAFSTLDWEVAQHVAQSIKRDPEHADDIARGKPSIDQIDASRRVTSIAFYTALLANLKAFEFALDAFEERLVERAGDSAPSFRQARDAFETVYRLAERFAREQGYTGSAPHAQAAPQAQPERVEPVFGQPIQTEETHVQQQTASRPPVTQMIAGIQNRAQAVDQLRAVARYFRQTEPHSPVAYLADKAAEWADMPLHKWLESVVKDDGSLSHIRELLGVRPDEQA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9813 - -


  Protein sequence: 374 a.a.    

>T6CP001218 NC_008060:c2897635-2896514 [Burkholderia cenocepacia AU 1054] [TssA]
MPINLPELLTPISDASPSGDDLLFSNEFDAIQDARRYDDPTLDQGEWVTEIKEADWGFVVDHAGELLRTRTKDLRLAVWL
TEALALEDGITGLTEGYALLEGLCRDFWDTFHPLPEDDDIEHRLGNVAWLSGRTAELLRAVPLTDGASNAFSTLDWEVAQ
HVAQSIKRDPEHADDIARGKPSIDQIDASRRVTSIAFYTALLANLKAFEFALDAFEERLVERAGDSAPSFRQARDAFETV
YRLAERFAREQGYTGSAPHAQAAPQAQPERVEPVFGQPIQTEETHVQQQTASRPPVTQMIAGIQNRAQAVDQLRAVARYF
RQTEPHSPVAYLADKAAEWADMPLHKWLESVVKDDGSLSHIRELLGVRPDEQA*

  Nucleotide sequence: 1122 bp    

>T6CP001218 NC_008060:c2897635-2896514 [Burkholderia cenocepacia AU 1054] [TssA]
ATGCCGATCAATCTCCCCGAGCTGCTGACACCGATCAGCGATGCGTCGCCCAGCGGCGACGACCTGCTGTTTTCGAACGA
ATTCGACGCGATCCAGGACGCGCGGCGCTACGACGACCCGACGCTCGACCAGGGCGAATGGGTAACCGAGATCAAGGAGG
CCGACTGGGGCTTCGTCGTCGACCATGCGGGCGAGCTGCTGCGCACCCGCACCAAGGACCTGCGGCTCGCCGTGTGGCTG
ACCGAGGCGCTCGCGCTCGAGGACGGCATCACCGGCCTCACCGAAGGCTATGCGCTGCTCGAAGGCCTGTGCCGCGACTT
CTGGGACACCTTTCACCCGCTGCCCGAGGACGACGACATCGAGCACCGGCTCGGCAACGTCGCGTGGCTGTCCGGCCGCA
CGGCCGAGCTGCTGCGCGCGGTGCCGCTGACGGACGGCGCATCGAATGCGTTCAGCACGCTCGATTGGGAAGTCGCGCAG
CACGTCGCGCAGTCGATCAAGCGCGACCCCGAACATGCCGACGACATCGCGCGCGGCAAGCCGTCGATCGACCAGATCGA
CGCGTCGCGGCGCGTGACGTCGATCGCGTTCTACACGGCGCTGCTGGCGAACTTGAAGGCGTTCGAATTCGCGCTCGATG
CGTTCGAGGAGCGGCTCGTCGAGCGCGCGGGCGATTCGGCGCCGAGCTTCCGGCAGGCGCGCGACGCGTTCGAGACCGTG
TACCGGCTCGCCGAGCGCTTTGCGCGCGAACAGGGCTATACGGGCAGCGCGCCGCACGCGCAGGCGGCGCCGCAGGCCCA
GCCCGAACGCGTCGAGCCGGTGTTCGGCCAGCCGATCCAGACCGAGGAGACTCACGTGCAGCAGCAGACCGCTTCGCGTC
CGCCGGTGACGCAGATGATCGCCGGCATCCAGAACCGCGCGCAGGCCGTCGACCAGTTGCGCGCGGTGGCGCGCTATTTC
CGTCAGACCGAGCCGCACAGCCCGGTCGCGTATCTTGCCGACAAGGCGGCCGAGTGGGCCGACATGCCGCTGCACAAGTG
GCTCGAGAGCGTCGTGAAGGACGACGGCTCGCTGTCGCACATTCGCGAGCTGCTGGGGGTGAGGCCCGACGAGCAGGCGT
GA