SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001061
Component protein type	TssI
T6SS ID (Type)	T6SS00112 (Type i3)
Strain	Burkholderia thailandensis E264
Replicon	chromosome II
Sequence	Protein sequence (735 a.a.); Nucleotide sequence (2205 bp)
Reference	[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806 [2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BTH_RS01375
Coordinate (Strand)	326355..328559 (+)
NCBI ID	WP_009895034.1
RefSeq	NC_007650
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	3.6e-102	23..325
Phage_base_V	PF04717.14	1.9e-14	379..448
Gp5_C	PF06715.14	8.2e-06	556..579

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-734	VTRVFTLDSLHGDDLKFHRLYGEEALGRMFDFRIEALADNHSLSLKELLGKPVTVRIRQQDESERHLNGIVARAALVGRRAQRHYGYQLIVRPWLWLATRRSDCRIFQNKTVPEIVQDVLVTYGFPIENHLTDTYAPRDYCVQYNETDAAFVSRLMEFEGIYYYFKHAAQTHTLMLCDAMASHVALPGYEHIPFIARDRTAIADEEHIDSWLPAQEVSIGKHETSDYDYTKPRADLSAQKIDPRGHDHDGFASFEWPGGYRDDEPGAHYSRVRLEEQQAEHERALARTDVRGIAPGYLFTLEHCPRADQNREYLIVRCQYRFQENAYATDSGNEAVVHESQVLVQPSSLPYRSPRATPRPRTNGPQTATVVGPVGEEIWTDQYGRVKLQFRWDRYGQSDQNSSCWVRVSSPWAGGGFGGVQIPRIGDEVVVDFLNGDPDQPIVTGRVYNGEKMPPWGLPGSATQSGLLSRSSPGGTTEHSNAFRFEDKKGAEQLWMHAERNFDAETEQDHTLSVGHDHSHSVGNNETMSVANDRQRSVGQNETVNIGKHRVAQIGGNETHGVAGNRTRQVGQNEAVTIGANREATIGGNHVETVAKDKTETIGQGKTLNVTQHYQTNSKSMKTAVVQHHAEEIGSRTSTIKNAHVLNVGDSQSVNVGASHTMSVRNNVHVGAGDEIALVCGHASITLKKDGTILINGVTVESSASGSHSVRGKTVTSSATGEHTVEGTILKLNP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9955	-	-

Protein sequence: 735 a.a.

						>T6CP001061 NC_007650:326355-328559 [Burkholderia thailandensis E264] [TssI]
VTRVFTLDSLHGDDLKFHRLYGEEALGRMFDFRIEALADNHSLSLKELLGKPVTVRIRQQDESERHLNGIVARAALVGRR
AQRHYGYQLIVRPWLWLATRRSDCRIFQNKTVPEIVQDVLVTYGFPIENHLTDTYAPRDYCVQYNETDAAFVSRLMEFEG
IYYYFKHAAQTHTLMLCDAMASHVALPGYEHIPFIARDRTAIADEEHIDSWLPAQEVSIGKHETSDYDYTKPRADLSAQK
IDPRGHDHDGFASFEWPGGYRDDEPGAHYSRVRLEEQQAEHERALARTDVRGIAPGYLFTLEHCPRADQNREYLIVRCQY
RFQENAYATDSGNEAVVHESQVLVQPSSLPYRSPRATPRPRTNGPQTATVVGPVGEEIWTDQYGRVKLQFRWDRYGQSDQ
NSSCWVRVSSPWAGGGFGGVQIPRIGDEVVVDFLNGDPDQPIVTGRVYNGEKMPPWGLPGSATQSGLLSRSSPGGTTEHS
NAFRFEDKKGAEQLWMHAERNFDAETEQDHTLSVGHDHSHSVGNNETMSVANDRQRSVGQNETVNIGKHRVAQIGGNETH
GVAGNRTRQVGQNEAVTIGANREATIGGNHVETVAKDKTETIGQGKTLNVTQHYQTNSKSMKTAVVQHHAEEIGSRTSTI
KNAHVLNVGDSQSVNVGASHTMSVRNNVHVGAGDEIALVCGHASITLKKDGTILINGVTVESSASGSHSVRGKTVTSSAT
GEHTVEGTILKLNP*					

Nucleotide sequence: 2205 bp

						>T6CP001061 NC_007650:326355-328559 [Burkholderia thailandensis E264] [TssI]
GTGACCCGTGTGTTCACACTCGACAGCCTGCACGGCGACGACCTGAAGTTCCATCGCCTCTACGGCGAGGAAGCGCTCGG
GCGGATGTTCGACTTCCGGATCGAAGCGCTCGCCGACAATCACAGCCTGTCGCTGAAGGAACTGCTCGGCAAGCCGGTGA
CGGTGCGCATCCGGCAGCAGGACGAATCGGAGCGCCATCTGAACGGGATCGTCGCGCGCGCCGCGCTGGTCGGGCGGCGC
GCGCAGCGGCATTACGGCTATCAACTGATCGTGCGGCCGTGGCTGTGGCTCGCGACGCGCCGCTCCGATTGCCGGATCTT
CCAGAACAAGACGGTGCCGGAGATCGTGCAGGACGTGCTCGTCACGTACGGCTTTCCGATCGAAAACCATCTGACCGACA
CGTACGCGCCGCGCGACTACTGCGTGCAGTACAACGAGACCGACGCGGCGTTCGTGTCGCGGCTGATGGAGTTCGAAGGG
ATCTACTACTACTTCAAGCACGCCGCGCAAACGCACACGCTGATGCTGTGCGACGCGATGGCGTCGCACGTCGCGCTGCC
GGGCTACGAGCACATTCCGTTCATTGCGCGCGACCGCACCGCGATCGCCGACGAAGAGCACATCGACAGCTGGCTGCCCG
CGCAGGAAGTGAGCATCGGCAAGCACGAGACGAGCGACTACGACTACACGAAGCCGCGCGCGGATCTGTCCGCGCAGAAG
ATCGATCCGCGCGGCCACGACCATGACGGCTTCGCGTCGTTCGAATGGCCCGGCGGCTATCGCGACGATGAACCGGGCGC
GCATTACAGCCGCGTGCGGCTCGAAGAGCAGCAAGCGGAGCACGAACGCGCGCTCGCGCGCACCGACGTGCGCGGCATCG
CACCCGGCTATCTGTTCACGCTCGAACACTGCCCGCGCGCCGACCAGAACCGCGAATACCTGATCGTGCGCTGCCAGTAT
CGGTTTCAGGAGAACGCGTACGCGACCGACAGCGGAAACGAAGCGGTCGTGCACGAATCGCAGGTGCTCGTGCAGCCTTC
GAGCCTGCCGTATCGCTCGCCGCGCGCAACGCCGCGGCCGCGCACGAACGGCCCGCAGACGGCGACCGTCGTCGGCCCGG
TGGGAGAAGAAATCTGGACGGACCAGTACGGGCGCGTGAAGCTGCAATTTCGCTGGGACCGCTACGGGCAGAGCGACCAG
AACTCATCGTGCTGGGTGCGCGTGTCGAGCCCGTGGGCGGGCGGCGGCTTCGGCGGCGTGCAGATCCCGCGCATCGGCGA
TGAAGTGGTGGTCGATTTTCTGAATGGCGATCCGGATCAGCCGATCGTGACGGGGCGCGTGTACAACGGCGAGAAGATGC
CGCCTTGGGGGTTGCCCGGCAGCGCGACGCAAAGCGGGCTGCTGTCGCGCTCGTCGCCGGGCGGCACGACCGAGCATTCG
AACGCGTTTCGCTTCGAGGACAAGAAGGGCGCGGAGCAACTGTGGATGCACGCGGAACGCAACTTCGACGCGGAAACCGA
GCAGGACCACACGCTGTCGGTGGGCCACGATCACTCGCATTCGGTCGGCAACAACGAGACGATGAGCGTCGCGAACGACC
GGCAGCGGAGCGTCGGGCAGAACGAGACGGTGAACATCGGGAAACATCGCGTCGCGCAGATCGGCGGGAACGAGACGCAT
GGCGTCGCGGGCAATCGCACGCGGCAGGTCGGACAGAACGAGGCCGTGACGATCGGCGCGAATCGCGAGGCGACGATCGG
CGGCAATCACGTGGAGACGGTCGCGAAAGACAAGACCGAAACGATCGGGCAGGGCAAGACGCTGAACGTCACCCAGCACT
ACCAGACGAATTCGAAAAGCATGAAGACCGCCGTCGTCCAACATCACGCCGAGGAGATCGGCTCGCGAACGTCGACGATC
AAGAACGCGCACGTGCTCAATGTCGGTGATTCGCAATCGGTGAACGTTGGCGCAAGCCATACGATGAGTGTGAGGAACAA
CGTGCATGTCGGCGCTGGCGATGAAATCGCACTCGTTTGCGGCCACGCGAGCATCACGCTGAAGAAGGACGGCACGATCT
TGATCAACGGCGTCACGGTGGAATCGAGCGCCAGCGGAAGCCATAGCGTGCGCGGCAAGACGGTGACGTCGTCGGCGACG
GGCGAGCACACGGTGGAAGGCACGATACTGAAACTGAACCCCTGA					