Detailed information of component protein


Summary

Component ID T6CP001051
Component protein type TssL
T6SS ID (Type) T6SS00112 insolico (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (438 a.a.); Nucleotide sequence (1314 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS01315
Coordinate (Strand) 307535..308848 (+)
NCBI ID WP_009895008.1
RefSeq NC_007650
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.4e-74 49..249
OmpA PF00691.22 7.9e-13 317..415
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-229 MNTSSDSFSAGTGGFVPPNPGGALPAAAPASAAASQPRPGRWAASGTNPLVAAANPLLNLVPQIRSTVHHPNPAWLREHLVVEIRQFEERAQQAGVASEAIIGARYCLCTALDEAAALTPWGGSVWSSHSLLVSFHNETWGGEKFFHLLERLSQQPRQHLDLLELLYFCLALGFEGRYRVLDNGRTQLDAVRRQLAQTIRSVRGEFDPALSPHWRDVVTRDVTRRFTVP
Transmembrane helix 230-252 LWVCVALALLVGFGVFAGLRIAL
Outside 253-437 AGHSDRLFASIDALHVPKLEPAQPAPRPAPAPRVAKFLEPEIAAGLVSVRDEADRSVIVLRGDGLFGSGSTSVIDRYMPVLTRIADALNQVPGSVRVSGYTDDTPVHTARFASNWDLSRERAEAVRGLIAARLDRPDRITAEGRGTLDPVAPNDSPANRARNRRVEITLMLAPGGDAARAAKEAP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5450 - -


  Protein sequence: 438 a.a.    

>T6CP001051 NC_007650:307535-308848 [Burkholderia thailandensis E264] [TssL]
MNTSSDSFSAGTGGFVPPNPGGALPAAAPASAAASQPRPGRWAASGTNPLVAAANPLLNLVPQIRSTVHHPNPAWLREHL
VVEIRQFEERAQQAGVASEAIIGARYCLCTALDEAAALTPWGGSVWSSHSLLVSFHNETWGGEKFFHLLERLSQQPRQHL
DLLELLYFCLALGFEGRYRVLDNGRTQLDAVRRQLAQTIRSVRGEFDPALSPHWRDVVTRDVTRRFTVPLWVCVALALLV
GFGVFAGLRIALAGHSDRLFASIDALHVPKLEPAQPAPRPAPAPRVAKFLEPEIAAGLVSVRDEADRSVIVLRGDGLFGS
GSTSVIDRYMPVLTRIADALNQVPGSVRVSGYTDDTPVHTARFASNWDLSRERAEAVRGLIAARLDRPDRITAEGRGTLD
PVAPNDSPANRARNRRVEITLMLAPGGDAARAAKEAP*

  Nucleotide sequence: 1314 bp    

>T6CP001051 NC_007650:307535-308848 [Burkholderia thailandensis E264] [TssL]
ATGAACACTTCTTCCGATTCGTTCTCGGCCGGCACGGGCGGATTCGTGCCGCCGAACCCGGGCGGCGCGTTGCCGGCCGC
CGCGCCCGCGTCCGCGGCCGCGTCGCAGCCGCGGCCGGGCCGGTGGGCGGCGAGCGGCACGAATCCGCTCGTCGCGGCCG
CGAATCCGCTGCTGAACCTCGTGCCGCAGATTCGCTCGACGGTCCATCATCCGAATCCCGCGTGGCTGCGCGAGCATCTC
GTCGTCGAGATCCGCCAGTTCGAGGAGCGCGCGCAGCAGGCGGGCGTCGCGTCCGAGGCGATCATCGGCGCGCGCTACTG
CCTGTGCACGGCGCTCGACGAGGCGGCCGCGCTGACGCCGTGGGGCGGCAGCGTGTGGTCGTCGCACAGTCTGCTCGTGT
CGTTCCACAACGAGACGTGGGGCGGCGAGAAGTTCTTCCATCTGCTGGAGCGGCTGTCGCAGCAGCCGCGCCAGCATCTC
GACCTGCTCGAGCTGCTGTACTTCTGCCTCGCGCTCGGCTTCGAGGGGCGCTATCGCGTGCTCGACAACGGCCGCACGCA
GCTCGACGCGGTGCGCCGCCAGCTCGCGCAGACGATCCGCTCGGTGCGCGGCGAGTTCGATCCGGCGCTCTCGCCGCATT
GGCGCGACGTCGTCACGCGCGACGTCACGCGGCGCTTCACGGTGCCGCTGTGGGTGTGCGTCGCGCTCGCGCTGCTCGTG
GGCTTCGGCGTGTTCGCGGGGCTGCGCATCGCGCTCGCCGGGCATTCGGACCGGCTGTTCGCATCGATCGACGCGCTGCA
CGTGCCGAAGCTGGAGCCGGCGCAGCCCGCGCCGCGTCCGGCGCCCGCGCCGCGCGTCGCGAAGTTCCTCGAGCCGGAGA
TCGCCGCGGGGCTCGTGAGCGTGCGCGACGAAGCCGACCGCAGCGTGATCGTGCTGCGCGGCGACGGCCTGTTCGGGTCC
GGCTCGACCTCGGTGATCGATCGCTACATGCCGGTGCTCACGCGCATCGCCGACGCGCTGAACCAGGTGCCGGGCAGCGT
GCGCGTGAGCGGCTACACCGACGACACGCCGGTGCACACCGCGCGCTTCGCGTCGAACTGGGACTTGTCGCGCGAGCGCG
CGGAGGCGGTCCGCGGCCTGATCGCCGCGCGGCTCGACCGTCCGGACCGGATCACGGCCGAAGGGCGCGGCACGCTCGAT
CCGGTCGCGCCGAACGATTCGCCGGCGAACCGCGCGCGCAACCGGCGCGTCGAGATCACGCTGATGCTCGCGCCCGGCGG
CGACGCCGCGCGCGCGGCGAAGGAGGCGCCCTGA