SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001049
Component protein type	TssJ
T6SS ID (Type)	T6SS00112 (Type i3)
Strain	Burkholderia thailandensis E264
Replicon	chromosome II
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806 [2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BTH_RS01305
Coordinate (Strand)	305598..306116 (+)
NCBI ID	WP_009895004.1
RefSeq	NC_007650
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	2.2e-35	31..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MRLRFSGSVALGGALLLAGCGATERSVAVPYSIALDVAPDVNPDINRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSVLGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPSEMKLQVAVKNGGIGLGGDSRVRR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9984	pos: 19-20. LAG-CG.	MRLRFSGSVALGGALLLAG

Protein sequence: 173 a.a.

						>T6CP001049 NC_007650:305598-306116 [Burkholderia thailandensis E264] [TssJ]
MRLRFSGSVALGGALLLAGCGATERSVAVPYSIALDVAPDVNPDINRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSV
LGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPSEMKLQVAVKNG
GIGLGGDSRVRR*					

Nucleotide sequence: 519 bp

						>T6CP001049 NC_007650:305598-306116 [Burkholderia thailandensis E264] [TssJ]
ATGCGACTGCGATTCAGTGGTTCCGTCGCGCTGGGAGGCGCGCTGCTGCTCGCCGGGTGCGGTGCGACCGAGCGTTCCGT
TGCGGTGCCGTATTCGATCGCGCTCGACGTCGCGCCCGACGTCAATCCGGACATCAATCGCAAGCCGTCGCCGATCGTGC
TGAAGGTGTTCCAGTTGAAGACGGCGTCCGCGTTCGAGAGCGCCGATTTCTTCTCGCTGCAGGACAAGCCGCAGAGCGTG
CTCGGCGCGGATCTGCTCGGCGTCGACCGGATCATCCTGCGGCCGGGCGATGCGCGCACGCTGCACTATCGCGGCAACGT
CGACGCGGGCGCGATCGGCATCGTCGCCGAATACCGCGTGCTCGAGAAGAACCGCTGGCGGATGACGGTGCCGCTGCCGC
GCGCGAAGCAACTGAACCTGTACCGATTCTGGCAAACCTCGCCGAGCGAGATGAAGCTGCAGGTCGCCGTGAAGAACGGC
GGCATCGGCCTCGGCGGCGATTCGCGAGTACGTCGATGA					