SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP001044
Component protein type	TssA
T6SS ID (Type)	T6SS00111 (Type i3)
Strain	Burkholderia thailandensis E264
Replicon	chromosome II
Sequence	Protein sequence (357 a.a.); Nucleotide sequence (1071 bp)
Reference	[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806 [2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BTH_RS00685
Coordinate (Strand)	160068..161138 (-)
NCBI ID	WP_009894751.1
RefSeq	NC_007650
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	8.4e-34	16..139

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-356	MNATTPAATLRYADLLAPVSQDAPCGPDLEYDPAFVMLQSAIAPKKDAQYGEFVEAPQPANWAEAERDCRALLLRTKDIRLVVILTRCRIRQSGAQGLRDGLALLNEMLARYGEALHPVPFFEGERDPVVYANAIATLADPDATLADIREIQLPKASGLQLQLRDIEKALAVTRVKDALAPESASRLLKEWWNRRDGTIVALAQAQCLMADLIASTRESLGDDAPDLSGIAKLLHPFTQAQLESPYSASAAQPQGDAKPANGDTAHSRAADALAPAGDAATQTPAAMSIANPQPPMDRRGALAAIQATRLWFEQNEPSSPVIVLLRQSERMVGKRFSEIANAIPAELLAQWDALDV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8850	-	-

Protein sequence: 357 a.a.

						>T6CP001044 NC_007650:c161138-160068 [Burkholderia thailandensis E264] [TssA]
MNATTPAATLRYADLLAPVSQDAPCGPDLEYDPAFVMLQSAIAPKKDAQYGEFVEAPQPANWAEAERDCRALLLRTKDIR
LVVILTRCRIRQSGAQGLRDGLALLNEMLARYGEALHPVPFFEGERDPVVYANAIATLADPDATLADIREIQLPKASGLQ
LQLRDIEKALAVTRVKDALAPESASRLLKEWWNRRDGTIVALAQAQCLMADLIASTRESLGDDAPDLSGIAKLLHPFTQA
QLESPYSASAAQPQGDAKPANGDTAHSRAADALAPAGDAATQTPAAMSIANPQPPMDRRGALAAIQATRLWFEQNEPSSP
VIVLLRQSERMVGKRFSEIANAIPAELLAQWDALDV*					

Nucleotide sequence: 1071 bp

						>T6CP001044 NC_007650:c161138-160068 [Burkholderia thailandensis E264] [TssA]
ATGAATGCCACCACACCCGCTGCAACGCTGCGCTACGCGGATCTTCTCGCGCCCGTCTCACAGGATGCGCCCTGCGGGCC
GGACCTCGAATACGATCCGGCGTTCGTGATGCTGCAATCCGCGATCGCGCCGAAGAAGGATGCGCAATATGGCGAGTTCG
TCGAAGCGCCTCAGCCCGCGAACTGGGCGGAGGCGGAACGCGACTGCCGCGCGCTGCTGTTGCGCACGAAGGACATTCGG
CTCGTCGTGATCCTGACACGATGCAGAATCCGCCAGAGCGGCGCGCAAGGGCTGCGCGACGGCCTCGCGCTACTCAACGA
GATGCTCGCGCGTTACGGCGAGGCGCTGCACCCTGTACCGTTCTTCGAAGGCGAACGCGATCCCGTGGTCTATGCGAACG
CGATCGCCACGCTTGCGGACCCCGACGCAACGCTTGCGGACATCCGGGAAATCCAGTTGCCCAAGGCGAGCGGCCTGCAA
TTGCAGTTGCGCGACATCGAAAAGGCGCTCGCGGTGACGCGCGTGAAAGACGCTCTCGCGCCCGAATCGGCCAGCCGGCT
GCTCAAGGAATGGTGGAACCGGCGCGACGGAACGATCGTCGCGCTCGCGCAGGCCCAGTGCCTCATGGCCGATTTGATCG
CCTCGACCCGTGAATCGCTCGGCGACGACGCGCCCGACCTGTCCGGCATCGCGAAACTGCTGCATCCGTTTACGCAGGCG
CAACTGGAATCGCCGTATTCGGCGTCCGCCGCTCAACCGCAAGGCGATGCGAAGCCGGCGAACGGCGACACCGCGCATTC
GCGCGCCGCCGACGCCCTCGCCCCGGCAGGCGACGCCGCCACGCAAACGCCCGCTGCAATGTCGATCGCAAACCCGCAAC
CGCCGATGGATCGACGGGGCGCGCTGGCGGCCATTCAGGCGACGCGCCTTTGGTTCGAGCAGAACGAGCCGAGCAGCCCG
GTAATCGTGCTGCTGCGCCAGTCGGAGCGCATGGTCGGCAAGCGCTTTTCGGAAATCGCGAATGCGATTCCCGCCGAGTT
GCTCGCTCAGTGGGATGCGCTCGACGTCTAG					