Detailed information of component protein


Summary

Component ID T6CP001041
Component protein type TssL
T6SS ID (Type) T6SS00111 insolico (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (434 a.a.); Nucleotide sequence (1302 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS00645
Coordinate (Strand) 145209..146510 (+)
NCBI ID WP_009894733.1
RefSeq NC_007650
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3.8e-62 63..266
OmpA PF00691.22 1.3e-16 327..425
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-244 MNSGAFDEAAFDFLASDEGRASVAADRARSAAKAAGAEPARAEIDASVNVQHRLDEVRAAANPLLEAANPLLRMLADMPAMLDTSEAVSSLRTLLVREVALFQNLCEKADLPWKHMAVVRYCLCTALDEAANRTRWGGGGVWASQSLLITYEGEVDGGEKFFLLIGRMATDPQEYVDILEILYRVLGLGFEGRYSVVADGRRHLEQIRQRLWTLITGARDAIQPELSPHWRGAEPGKLPLLRSV
Transmembrane helix 245-264 PAWATGSFVFLLLFGLFAFY
Inside 265-433 QYRLLTERNALEARILAIAKDEPASPQQRLRLSILLKNEIARGLLTVDEDDARSVVVFRGDSMFRSGESRVLPEIEPVLDKVAREVARVGGRVTVTGHSDNQPIRRADIPNNLVLSEKRAAYVAQILVQRGTPADHIRSEGRGDAQPVAENTTPAGRSRNRRVEIAVTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9414 - -


  Protein sequence: 434 a.a.    

>T6CP001041 NC_007650:145209-146510 [Burkholderia thailandensis E264] [TssL]
MNSGAFDEAAFDFLASDEGRASVAADRARSAAKAAGAEPARAEIDASVNVQHRLDEVRAAANPLLEAANPLLRMLADMPA
MLDTSEAVSSLRTLLVREVALFQNLCEKADLPWKHMAVVRYCLCTALDEAANRTRWGGGGVWASQSLLITYEGEVDGGEK
FFLLIGRMATDPQEYVDILEILYRVLGLGFEGRYSVVADGRRHLEQIRQRLWTLITGARDAIQPELSPHWRGAEPGKLPL
LRSVPAWATGSFVFLLLFGLFAFYQYRLLTERNALEARILAIAKDEPASPQQRLRLSILLKNEIARGLLTVDEDDARSVV
VFRGDSMFRSGESRVLPEIEPVLDKVAREVARVGGRVTVTGHSDNQPIRRADIPNNLVLSEKRAAYVAQILVQRGTPADH
IRSEGRGDAQPVAENTTPAGRSRNRRVEIAVTL*

  Nucleotide sequence: 1302 bp    

>T6CP001041 NC_007650:145209-146510 [Burkholderia thailandensis E264] [TssL]
ATGAACTCGGGCGCCTTTGACGAAGCCGCGTTCGATTTCCTTGCGTCGGACGAAGGCCGGGCGAGCGTCGCCGCGGATCG
CGCGCGATCCGCGGCGAAGGCCGCGGGCGCGGAGCCCGCGCGCGCCGAAATCGACGCATCGGTCAACGTCCAGCATCGCC
TCGACGAAGTTCGCGCGGCTGCGAATCCGCTCCTCGAAGCAGCGAATCCCTTGCTGCGGATGCTGGCGGACATGCCTGCG
ATGCTCGACACGTCCGAAGCCGTGTCGAGCCTTCGCACGCTGCTCGTGCGCGAGGTCGCGCTGTTCCAGAACTTGTGCGA
AAAGGCAGACCTGCCCTGGAAGCACATGGCGGTCGTGCGTTATTGCCTGTGCACGGCACTGGACGAGGCGGCCAATCGCA
CGCGTTGGGGCGGCGGCGGCGTATGGGCTTCGCAGAGCTTGCTGATTACCTACGAAGGCGAAGTCGACGGCGGCGAGAAG
TTCTTCCTGTTGATCGGACGCATGGCGACGGACCCGCAGGAGTACGTCGATATTCTCGAGATTCTCTATCGCGTGCTGGG
TCTCGGGTTCGAAGGACGCTACAGCGTCGTGGCGGACGGCCGGCGGCATCTCGAGCAGATTCGCCAGCGTCTGTGGACGC
TCATCACCGGCGCGCGCGACGCGATTCAGCCGGAGTTGTCGCCGCATTGGCGCGGAGCCGAGCCCGGCAAGCTTCCGCTG
TTGCGCAGCGTGCCCGCATGGGCGACAGGTTCGTTCGTCTTTCTGTTGCTGTTCGGTCTCTTCGCGTTCTATCAGTATCG
GCTGCTTACCGAACGCAACGCGCTCGAGGCCCGCATTCTGGCCATCGCGAAGGATGAGCCGGCTTCGCCGCAGCAGCGTT
TGCGCCTGTCGATATTGCTCAAGAACGAGATTGCGCGCGGTCTGTTGACGGTCGACGAAGACGACGCGCGCAGCGTCGTG
GTCTTCCGCGGCGATTCGATGTTCCGCTCCGGCGAGAGCCGCGTGCTTCCCGAGATCGAGCCGGTTCTCGACAAGGTGGC
CCGGGAAGTTGCCCGTGTCGGCGGCAGGGTCACGGTCACGGGGCACTCCGACAACCAGCCGATTCGGCGCGCCGATATCC
CGAACAACCTCGTGCTGTCGGAGAAACGCGCGGCTTACGTTGCGCAAATCCTGGTCCAGCGAGGCACACCGGCCGATCAC
ATTCGCTCGGAGGGCCGGGGCGACGCGCAGCCCGTCGCGGAGAACACGACGCCTGCTGGCCGATCCCGCAACCGCCGCGT
TGAAATCGCGGTCACGCTGTAA