SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000754
Component protein type	TssB
T6SS ID (Type)	T6SS00084 (Type i1)
Strain	Aliivibrio fischeri ES114
Replicon	chromosome I
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VF_RS05240
Coordinate (Strand)	1109176..1109676 (-)
NCBI ID	WP_005418723.1
RefSeq	NC_006840
Uniprot ID	Q5E647_ALIF1, B5FD90_ALIFM, A0A1B9PCJ4_ALIFS
KEGG ID	vfm:VFMJ11_1087, vfi:VF_1004
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	4.8e-54	6..159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MSKDGSVAPKERVNVTFKPVSGDAAEDLELPLKMMVLGDFTQQEDDRKLEDRKPFNVDKSNFNDVIEKQNLNISFGVKNQLQDDGEGNEEIAVDLSFKHINDFDPSSIVEQVPELKGLMDLREALVSLKGPLGNVPAFRKAIEDALVDDEAREKLLSELGLVTEKE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9942	-	-

Protein sequence: 167 a.a.

						>T6CP000754 NC_006840:c1109676-1109176 [Aliivibrio fischeri ES114] [TssB]
MSKDGSVAPKERVNVTFKPVSGDAAEDLELPLKMMVLGDFTQQEDDRKLEDRKPFNVDKSNFNDVIEKQNLNISFGVKNQ
LQDDGEGNEEIAVDLSFKHINDFDPSSIVEQVPELKGLMDLREALVSLKGPLGNVPAFRKAIEDALVDDEAREKLLSELG
LVTEKE*					

Nucleotide sequence: 501 bp

						>T6CP000754 NC_006840:c1109676-1109176 [Aliivibrio fischeri ES114] [TssB]
ATGTCAAAAGATGGATCTGTTGCACCAAAAGAACGTGTAAATGTGACATTCAAACCAGTAAGCGGTGACGCTGCAGAAGA
TCTTGAACTTCCACTGAAAATGATGGTTCTTGGTGACTTTACTCAGCAAGAAGATGATCGAAAACTTGAAGATCGAAAAC
CATTTAATGTTGATAAAAGCAATTTCAATGACGTTATTGAAAAACAAAACTTAAACATCTCTTTTGGTGTAAAAAACCAA
CTTCAAGATGATGGCGAAGGAAATGAGGAAATTGCAGTCGATCTAAGTTTTAAACATATCAATGATTTTGATCCTTCAAG
CATTGTTGAGCAAGTGCCTGAACTAAAAGGCTTAATGGATTTACGTGAAGCGCTTGTCTCTTTAAAAGGCCCATTAGGCA
ATGTTCCTGCATTCCGCAAAGCAATTGAAGATGCCCTTGTTGATGATGAAGCTCGTGAAAAACTACTAAGTGAATTAGGT
TTAGTGACAGAAAAGGAATAA					