SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000752
Component protein type	TssF
T6SS ID (Type)	T6SS00084 (Type i1)
Strain	Aliivibrio fischeri ES114
Replicon	chromosome I
Sequence	Protein sequence (584 a.a.); Nucleotide sequence (1752 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VF_RS05225
Coordinate (Strand)	1105434..1107185 (-)
NCBI ID	WP_011261634.1
RefSeq	NC_006840
Uniprot ID	Q5E650_ALIF1
KEGG ID	vfi:VF_1001
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	2.8e-127	3..580

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-583	MSFNHYYQSELAALRELGKSFSRENPALAPYLGQEGLDPDIERLFEGFAFLVGRLHQRLDNQLPELTHSLMRLLWPNYVQAIPAMSTLQFTPASEATQASIIKRGTEVLSQPIDGIQCKFKTCFDVDIYPVSLHSSSFTANGHGGIYQLRLELPSGIDLASLNMKQLRVHFNGDKASCLSLIMALCSSTTQAEFCALDKELEQINRSTFPPHSIKPVGFKTNEAIFDYPLNTFEGYRHIQEFFCYPEKYYYIDLTELPVWPAQFSQGCKYVDLKFTLDNITQQPFYANRVQPELFCTPIVNSFKSDTQPLLLNHRKDQYKITPVSLNDDSSTILSVKEVHGWNPGQKGRIDFYHFESFEHNENKNRSNYYSIRQEKNIVSGHFDTYLSFRSQHTFTNSVTVSIAVTACNGALAQRLSVNSITETTDQTTGSATFTNIRTVTPCYTPPIDKDGLWSLISNMSLNYLSLTKPEAFKRILLAYDFAGQQDDAKGKKHKRLIDGIVSISTKPCDMLYEGAPVRAMNTQLHLNTKHFLCEGELYLFSTVINEFLSLYTSMNSFNQLHVTSTDGGDYSWLPRMGQQPLI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9953	-	-

Protein sequence: 584 a.a.

						>T6CP000752 NC_006840:c1107185-1105434 [Aliivibrio fischeri ES114] [TssF]
MSFNHYYQSELAALRELGKSFSRENPALAPYLGQEGLDPDIERLFEGFAFLVGRLHQRLDNQLPELTHSLMRLLWPNYVQ
AIPAMSTLQFTPASEATQASIIKRGTEVLSQPIDGIQCKFKTCFDVDIYPVSLHSSSFTANGHGGIYQLRLELPSGIDLA
SLNMKQLRVHFNGDKASCLSLIMALCSSTTQAEFCALDKELEQINRSTFPPHSIKPVGFKTNEAIFDYPLNTFEGYRHIQ
EFFCYPEKYYYIDLTELPVWPAQFSQGCKYVDLKFTLDNITQQPFYANRVQPELFCTPIVNSFKSDTQPLLLNHRKDQYK
ITPVSLNDDSSTILSVKEVHGWNPGQKGRIDFYHFESFEHNENKNRSNYYSIRQEKNIVSGHFDTYLSFRSQHTFTNSVT
VSIAVTACNGALAQRLSVNSITETTDQTTGSATFTNIRTVTPCYTPPIDKDGLWSLISNMSLNYLSLTKPEAFKRILLAY
DFAGQQDDAKGKKHKRLIDGIVSISTKPCDMLYEGAPVRAMNTQLHLNTKHFLCEGELYLFSTVINEFLSLYTSMNSFNQ
LHVTSTDGGDYSWLPRMGQQPLI*					

Nucleotide sequence: 1752 bp

						>T6CP000752 NC_006840:c1107185-1105434 [Aliivibrio fischeri ES114] [TssF]
ATGAGTTTTAATCATTACTATCAATCTGAATTAGCAGCACTGCGCGAATTAGGAAAGTCGTTTTCGAGAGAAAATCCGGC
TTTAGCACCCTATTTAGGTCAAGAGGGATTGGATCCAGATATTGAGCGATTATTCGAAGGATTTGCTTTTTTAGTTGGTC
GTTTACATCAACGCTTAGATAACCAGTTACCAGAATTAACTCATTCATTAATGCGTTTATTGTGGCCTAACTATGTCCAA
GCAATTCCAGCAATGAGTACTCTGCAATTTACACCGGCAAGTGAAGCAACCCAAGCATCAATAATTAAACGTGGTACGGA
AGTACTTAGCCAACCTATTGATGGCATTCAATGTAAATTTAAAACATGTTTTGATGTTGATATTTATCCAGTATCACTTC
ATTCATCGTCTTTTACAGCAAATGGGCATGGTGGCATTTATCAATTACGATTGGAATTACCTTCAGGAATTGATCTAGCG
TCGTTAAATATGAAGCAATTACGTGTTCATTTTAATGGAGACAAAGCCAGCTGCTTATCTCTTATTATGGCGCTATGCTC
TTCCACTACTCAAGCTGAGTTTTGTGCTTTGGATAAAGAGTTAGAACAAATCAATCGCTCAACGTTTCCACCCCATAGCA
TTAAACCTGTTGGCTTTAAAACCAATGAGGCTATTTTTGATTACCCTCTCAATACATTTGAAGGCTACCGACACATTCAA
GAATTTTTTTGTTACCCAGAAAAATATTACTACATTGACTTAACTGAGTTACCAGTTTGGCCAGCTCAGTTTAGTCAAGG
GTGTAAATATGTCGATTTAAAATTCACGTTAGATAACATCACACAACAACCTTTTTACGCTAATCGTGTTCAACCAGAGC
TATTTTGTACCCCAATCGTTAATTCATTTAAAAGTGATACACAACCACTCTTACTTAACCACAGAAAAGATCAATATAAG
ATCACGCCAGTTTCTTTAAATGACGATAGCAGTACTATTTTATCTGTCAAAGAAGTACACGGTTGGAACCCTGGGCAAAA
AGGTCGTATCGATTTTTACCACTTTGAATCATTTGAACATAACGAAAATAAAAATCGCTCAAACTATTACAGTATTCGTC
AAGAAAAGAACATTGTCAGTGGGCACTTTGATACGTATCTGTCTTTTCGCTCTCAACACACTTTTACCAATAGCGTTACT
GTTTCTATTGCCGTTACTGCATGTAATGGGGCATTAGCTCAACGTTTATCTGTAAACTCAATTACAGAAACAACAGATCA
AACAACTGGTTCAGCCACATTTACCAATATCAGAACAGTAACGCCTTGCTACACCCCACCCATTGATAAAGATGGTCTTT
GGTCGCTCATATCAAATATGTCCCTTAATTATCTTTCATTAACCAAGCCTGAAGCGTTTAAACGTATTTTGCTTGCTTAT
GATTTTGCTGGTCAACAAGACGATGCAAAAGGAAAAAAACATAAAAGACTGATCGATGGCATCGTAAGTATATCGACCAA
ACCTTGCGATATGTTATATGAAGGTGCTCCTGTACGAGCAATGAATACACAACTTCATCTCAACACGAAGCACTTTTTAT
GTGAAGGCGAGCTGTATTTATTTTCCACTGTAATTAATGAATTTTTATCGCTTTATACCAGTATGAATTCATTCAACCAA
TTGCACGTTACAAGCACTGATGGAGGAGACTATTCATGGCTACCACGAATGGGGCAACAACCTCTAATTTAG					